New affine sorbent for determining glycosylated hemoglobin in early diagnostics of carbohydrate metabolism disturbances

Abstract

Data on the synthesis and properties of a new affine sorbent created on the basis of a modified poly(acrylamide) gel with immobilized m-aminophenylboronic acid are presented. The new sorbent can be used for a multiple (no less then 7 cycles) quantitative determination of compounds containing cis-diol groups, in particular, glycosylated hemoglobin. The proposed affine sorbent offers a promising basis for the creation of diagnostic screening systems of carbohydrate metabolism disturbances and for monitoring diabetes mellitus compensation. In a series of tests for glycosylated hemoglobin determination with the new sorbent, the variation coefficient did not exceed 5%.