New advances in animal transgenic technology

Sun, Zhenhong; Miao, Xiangyang; Zhu, Rui-Liang

doi:10.3724/sp.j.1005.2010.00539

Cited by 16 publications

(17 citation statements)

References 42 publications

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“…The first tetracycline (tet)-controlled transcriptional activation system was designed nearly a decade ago to control transgene expression temporally. The activation or suppression of a variety of genes in vivo has provided invaluable insights into the function and regulation of these genes in various tissues and cells (1)(2)(3)(4)(5)(6). The original tet-controlled transcriptional activator (tTA) is a transcriptional regulator with tight control of target gene expression and a broad range of inducibility (7)(8)(9)(10)(11)(12)(13).…”

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confidence: 99%

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Airway-Specific Inducible Transgene Expression Using Aerosolized Doxycycline

Tata

Pardo–Saganta²,

Prabhu³

et al. 2013

Am J Respir Cell Mol Biol

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Tissue-specific transgene expression using tetracycline (tet)-regulated promoter/operator elements has been used to revolutionize our understanding of cellular and molecular processes. However, because most tet-regulated mouse strains use promoters of genes expressed in multiple tissues, to achieve exclusive expression in an organ of interest is often impossible. Indeed, in the extreme case, unwanted transgene expression in other organ systems causes lethality and precludes the study of the transgene in the actual organ of interest. Here, we describe a novel approach to activating tet-inducible transgene expression solely in the airway by administering aerosolized doxycycline. By optimizing the dose and duration of aerosolized doxycycline exposure in mice possessing a ubiquitously expressed Rosa26 promoter-driven reverse tet-controlled transcriptional activator (rtTA) element, we induce transgene expression exclusively in the airways. We detect no changes in the cellular composition or proliferative behavior of airway cells. We used this newly developed method to achieve airway basal stem cell-specific transgene expression using a cytokeratin 5 (also known as keratin 5)-driven rtTA driver line to induce Notch pathway activation. We observed a more robust mucous metaplasia phenotype than in mice receiving doxycycline systemically. In addition, unwanted phenotypes outside of the lung that were evident when doxycycline was received systemically were now absent. Thus, our approach allows for rapid and efficient airway-specific transgene expression. After the careful strain by strain titration of the dose and timing of doxycycline inhalation, a suite of preexisting transgenic mice can now be used to study airway biology specifically in cases where transient transgene expression is sufficient to induce a phenotype.

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confidence: 99%

“…A nebulizer (1) is connected to a doxycycline reservoir (3) by tubing(2). The doxycycline reservoir is connected (4) to a microisolator chamber(5). The mouse is placed inside a 50-ml Falcon tube (REF 352070; BD Falcon, Franklin Lakes, NJ), with its snout snugly positioned through a 1-cm aperture in the tube (6).…”

mentioning

confidence: 99%

Airway-Specific Inducible Transgene Expression Using Aerosolized Doxycycline

Tata

Pardo–Saganta²,

Prabhu³

et al. 2013

Am J Respir Cell Mol Biol

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“…With the development of medical and biological science, the minipigs have subsequently gained regulatory acceptance in pharmacology, toxicity and basic researches [ 4 ]. Recent evidence suggests a increasing demand of high-quality minipigs, which can reduce the interference of some inapparent pathogens [ 15 ]. However, ensuring an adequate supply of high-quality minipigs may become a main challenge.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a special pathogen free Chinese Wuzhishan Minipigs Colony

et al. 2015

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To meet the increasing demands of specific pathogen free (SPF) minipigs in biomedical researches, 8 pregnant Chinese Wuzhishan minipigs (WZSP) sows with clear background were chosen to obtain SPF WZSP by hysterectomy. At 111 ± 2 days of the pregnancy, piglets were aseptically taken out from the sows and artificially suckled for 40 to 45 days in the positive isolators. Then, the piglets defined as F0 were transferred to barrier environment and fed with standard feeds. The original SPF colony was formed for breeding by selected piglets from F0 group of 6–8 months old. Biological characteristics of SPF WZSP were collected and further compared to those of conventional (CV) WZSP, including growth performance, reproductive performance, hematology and blood biochemistry, and major pathogens detection. As a result, 61 F0 piglets were obtained from 8 candidate sows, and 55 out of them survived. After strictly selection, 35 F0 piglets were used to form the original SPF colony, which produced 14 litters of SPF piglets defined as F1. Piglet survival rates, growth performance, and reproductive performance of SPF WZSP were similar to CV WZSP. Some hematology and blood biochemistry parameters showed significant differences between SPF and CV WZSP. Eighteen kinds of pathogens were identified to be free in F0 and F1 SPF colony by repeated pathogen detections. In conclusion, we established a satisfied SPF WZSP colony maintaining original characteristics, free of controlled diseases, and being proved to be a suitable laboratory animal.

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“…The number and density are gradually decreasing. If every suitable habitat patch were seen as an isolated island which is suitable for the survival of giant panda, then this law fits island species biology diffusion law [10]. Hence, migration and dispersal can increase the possibility of the permanence of giant panda in the patchy environment.…”

Section: Discussionmentioning

confidence: 99%

The effect of diffusion on giant pandas that live in complex patchy environments

Shi

Song

2015

NAMC

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Abstract. The habitat loss and fragmentation is almost the greatest threat to the survival of the wild giant panda. In this paper, we construct a mathematical model to consider the effect of diffusion on giant pandas that live in complex patchy environments. Our discussion includes the studying of a diffusive n-dimensional single species model, sufficient conditions are derived for the permanence and extinction of the giant panda species. Especially, we also discuss the situations of diffusion of giant pandas between two patches, and numerical simulations are presented to illustrate the results. Furthermore, we consider the existence, uniqueness, and global stability of the positive periodic solution of the n-dimensional single species model. The implications of these results are significant for giant panda conservation.

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New advances in animal transgenic technology

Cited by 16 publications

References 42 publications

Airway-Specific Inducible Transgene Expression Using Aerosolized Doxycycline

Airway-Specific Inducible Transgene Expression Using Aerosolized Doxycycline

Establishment of a special pathogen free Chinese Wuzhishan Minipigs Colony

The effect of diffusion on giant pandas that live in complex patchy environments

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