New adjuvant design using layered double hydroxide for production of polyclonal antibodies in radioimmunoassay techniques

Shafik, Hesham M.; Ayoub, S. M.; Ebeid, N. H.; Someda, H. H.

doi:10.1007/s10967-014-3155-5

Cited by 19 publications

(4 citation statements)

References 15 publications

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“…Therefore, engineering high sensitivity, selective, rapid, and efficient analytical methods for the quantitative determination of AFP level in human plasma is of great importance for cancer diagnosis. Until now, numerous analytical methods including enzyme-linked immunosorbent assay [10], electrochemiluminescence [11], surface plasmon resonance imaging [12], radioimmunoassay [13], fluorescence [14], and atomic absorption spectrometry [15] techniques have been suggested for the monitoring of AFP level. Although most of these methods have advantages such as broad detection range and low determination limit, they are not effective due to the high cost, long time of analysis, and even their complicated processes [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

et al. 2022

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The early diagnosis of major diseases such as cancer is typically a major issue for humanity. Human α-fetoprotein (AFP) as a sialylated glycoprotein is of approximately 68 kD molecular weight and is considered to be a key biomarker, and an increase in its level indicates the presence of liver, testicular, or gastric cancer. In this study, an electrochemical AFP immunosensor based on Fe 3 O 4 NPs@covalent organic framework decorated gold nanoparticles (Fe 3 O 4 NPs@COF/AuNPs) for the electrode platform and double-coated magnetic nanoparticles (MNPs) based on SiO 2 @TiO 2 (MNPs@SiO 2 @TiO 2 ) nanocomposites for the signal amplification was fabricated. The immobilization of anti-AFP capture antibody was successfully performed on Fe 3 O 4 NPs@COF/AuNPs modified electrode surface by amino-gold affinity, while the conjugation of anti-AFP secondary antibody on MNPs@SiO 2 @TiO 2 was achieved by the electrostatic/ionic interactions. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) analysis, cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) techniques were used to characterize the nanostructures in terms of physical and electrochemical features. The limit of detection (LOD) was 3.30 fg mL −1 . The findings revealed that the proposed electrochemical AFP immunosensor can be effectively used to diagnose cancer.

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Section: Introductionmentioning

confidence: 99%

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

et al. 2022

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“…The AFP level in healthy human serum is less than 25 ng ml -1 , but it rises dramatically in individuals with liver cancer ( Jiang et al., 2010 ; Liu et al., 2011 ; Su et al., 2011 ). In general, enzyme-linked immunosorbent assay (ELISA) ( Darwish et al., 2013 ), radioimmunoassay ( Shafik et al., 2014 ), chemiluminescence immunoassay ( Fu et al., 2006 ), and electrochemical immunoassay ( Yuan et al, 2017 ) can be employed to detect such biomarkers. The first three standard immunoassays, on the other hand, have several disadvantages.…”

Section: Introductionmentioning

confidence: 99%

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Chanarsa

Jakmunee

Ounnunkad

2022

Front. Cell. Infect. Microbiol.

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Liver cancer is one of the most common global health problems that features a high mortality rate. Alpha-fetoprotein (AFP) is a potential liver cancer biomarker for the diagnosis of liver cancer. The quantitative detection of AFP at an ultratrace level has important medical significance. Using the reaction of the antibody–antigen pair in an immunosensor enables the sensitive and selective AFP assay. Finding a strategy in signal generation and amplification is challenging to fabricate new sensitive electrochemical immunosensors for AFP detection. This study demonstrates the construction of a simple, reliable, and label-free immunosensor for the detection of AFP on a smart phone. Exfoliated two-dimensional (2D) molybdenum diselenide (MoSe2) and 2D tungsten diselenide (WSe2) were employed to modify the disposable screen-printed carbon electrode (SPCE) to use as the electrochemical platform, which is affixed to a small potentiostat connected to a smart phone. The modified electrode offers antibody immobilization and allows detection of AFP via an immunocomplex forming a sandwich-like configuration with the AFP-corresponding aptamer. A heterojunction 2D MoSe2/2D WSe2 composite improves the SPCE’s reactivity and provides a large surface area and good adsorption capacity for the immobilizing antibodies. The signal generation for the immunosensor is from the electrochemical response of methylene blue (MB) intercalating into the aptamer bound on the electrode. The response for the proposed sandwich-like immunosensor is proportional to the AFP concentration (1.0–50,000 pg ml-1). The biosensor has potential for the development of a simple and robust point-of-care diagnostic platform for the clinical diagnosis of liver cancer, achieving a low limit of detection (0.85 pg ml-1), high sensitivity, high selectivity, good stability, and excellent reproducibility.

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“…To this end, a variety of methods for detecting AFP have been developed, such as enzyme-linked immunosorbent assay [ 12 ], radioimmunoassay [ 13 ], fluorescence immunoassay [ 14 ], electrochemiluminescence [ 15 ], Raman spectroscopy [ 16 ], electrochemical immunosensor [ 17 ], and so on. Although the enzyme-linked immunosorbent assay has the advantages of simple operation, no pollution to the environment, and, specifically, it cannot provide quantitative data and only has a narrow linear range.…”

Section: Introductionmentioning

confidence: 99%

Detection of Alpha-Fetoprotein Using Aptamer-Based Sensors

et al. 2022

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Alpha-fetoprotein (AFP) is widely-known as the most commonly used protein biomarker for liver cancer diagnosis at the early stage. Therefore, developing the highly sensitive and reliable method of AFP detection is of essential demand for practical applications. Herein, two types of aptamer-based AFP detection methods, i.e., optical and electrochemical biosensors, are reviewed in detail. The optical biosensors include Raman spectroscopy, dual-polarization interferometry, resonance light-scattering, fluorescence, and chemiluminescence. The electrochemical biosensors include cyclic voltammetry, electrochemical impedance spectroscopy, and giant magnetic impedance. Looking into the future, methods for AFP detection that are high sensitivity, long-term stability, low cost, and operation convenience will continue to be developed.

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New adjuvant design using layered double hydroxide for production of polyclonal antibodies in radioimmunoassay techniques

Cited by 19 publications

References 15 publications

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

Electrochemical α-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Detection of Alpha-Fetoprotein Using Aptamer-Based Sensors

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