New Activities of the Nuclear Pore Complexes

Wong, Richard W.

doi:10.3390/cells10082123

Cited by 11 publications

(9 citation statements)

References 59 publications

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“…2, C to K, and fig. S5) [other nucleoporins at the centrosome are available in ( 26 )] was confirmed in cultured cells and human fetal cortex samples (fig. S5, I and J).…”

Section: Resultsmentioning

confidence: 83%

Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity

O'Neill

Uzbas

Antognolli

et al. 2022

Science

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The centrosome provides an intracellular anchor for the cytoskeleton, regulating cell division, cell migration, and cilia formation. We used spatial proteomics to elucidate protein interaction networks at the centrosome of human induced pluripotent stem cell–derived neural stem cells (NSCs) and neurons. Centrosome-associated proteins were largely cell type–specific, with protein hubs involved in RNA dynamics. Analysis of neurodevelopmental disease cohorts identified a significant overrepresentation of NSC centrosome proteins with variants in patients with periventricular heterotopia (PH). Expressing the PH-associated mutant pre-mRNA-processing factor 6 (PRPF6) reproduced the periventricular misplacement in the developing mouse brain, highlighting missplicing of transcripts of a microtubule-associated kinase with centrosomal location as essential for the phenotype. Collectively, cell type–specific centrosome interactomes explain how genetic variants in ubiquitous proteins may convey brain-specific phenotypes.

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“…2, C to K, and fig. S5) [other nucleoporins at the centrosome are available in ( 26 )] was confirmed in cultured cells and human fetal cortex samples (fig. S5, I and J).…”

Section: Resultsmentioning

confidence: 83%

Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity

O'Neill

Uzbas

Antognolli

et al. 2022

Science

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“…There are numerous studies in the literature that analyze the relationship between NPCs and cancer; however, the number of studies that analyze the relationship between POM121, a nucleoporin, and leukemia is limited, and the relationship between POM121 and leukemogenesis could not be demonstrated [10][11][12]19 .…”

Section: Discussionmentioning

confidence: 99%

Expression of nuclear pore protein POM121 in childhood acute lymphoblastic leukemias and its relationship with prognosis

CAVLAK

Özkan²,

Bayram

et al. 2023

Cukurova Medical Journal

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Purpose: We aimed to investigate the status of POM121 gene expression, which is one of the nuclear pore proteins in childhood acute lymphoblastic leukemias (ALL), compared with the normal population, and its relationship with prognosis and other clinical findings. Materials and Methods: Fifty-nine patients with ALL followed up and treated between January 2008 and November 2013, and 36 control subjects were included in the study. A real-time PCR method was used to detect POM121 gene expressions. Results: The mean value of POM121 expression was 3.75±2.91 in ALL patients and 3.32±3.76 in the control group. The 3 and 10 year overall survival (OS) was better in ALL with lower POM121 expression (77%, 70% versus 68%, 58%, respectively). Although the OS was better in B-ALL patients, with lower POM121 expression (84%, 75% versus 54%, 46%, respectively), in T-ALL, in contrast, the OS results were better in patients with a higher POM121 expression (90%, 90% versus 60%, 60%, respectively). Patients with a higher POM121 expression than the mean of the control group and who had relapse and central nervous system involvement had statistically significantly lower OS results in the 3rd and 10th years (16%, 0% versus 84%, 78%, respectively). Conclusion: High POM121 expression negatively affects the prognosis in patients with ALL. This is a study to show the relationship between POM121 expression and prognosis in childhood acute lymphoblastic leukemias, POM121 function will be clarified further with more comprehensive studies.

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“…The nucleolus is composed of hundreds of copies of ribosomal genes, newly synthesized ribosomal RNA (rRNA), ribosomal proteins, and ribonucleoproteins. Other droplets found in the nucleus are listed in Table 2 [ 8 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 ].…”

Section: Biomolecular Droplets and Their Functionsmentioning

confidence: 99%

Biomolecular Liquid–Liquid Phase Separation for Biotechnology

Shil

Tsuruta

Kawauchi

et al. 2023

BioTech

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The liquid–liquid phase separation (LLPS) of biomolecules induces condensed assemblies called liquid droplets or membrane-less organelles. In contrast to organelles with lipid membrane barriers, the liquid droplets induced by LLPS do not have distinct barriers (lipid bilayer). Biomolecular LLPS in cells has attracted considerable attention in broad research fields from cellular biology to soft matter physics. The physical and chemical properties of LLPS exert a variety of functions in living cells: activating and deactivating biomolecules involving enzymes; controlling the localization, condensation, and concentration of biomolecules; the filtration and purification of biomolecules; and sensing environmental factors for fast, adaptive, and reversible responses. The versatility of LLPS plays an essential role in various biological processes, such as controlling the central dogma and the onset mechanism of pathological diseases. Moreover, biomolecular LLPS could be critical for developing new biotechnologies such as the condensation, purification, and activation of a series of biomolecules. In this review article, we introduce some fundamental aspects and recent progress of biomolecular LLPS in living cells and test tubes. Then, we discuss applications of biomolecular LLPS toward biotechnologies.

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New Activities of the Nuclear Pore Complexes

Cited by 11 publications

References 59 publications

Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity

Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity

Expression of nuclear pore protein POM121 in childhood acute lymphoblastic leukemias and its relationship with prognosis

Biomolecular Liquid–Liquid Phase Separation for Biotechnology

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