New 24-Membered Macrolactins Isolated from Marine Bacteria <i>Bacillus siamensis</i> as Potent Fungal Inhibitors against Sugarcane Smut

Gao, Chenghai; Chen, Xianqiang; Yu, Lian; Jiang, Lei; Pan, Dongjin; Jiang, Shengyang; Gan, Yuman; Liu, Yonghong; Yi, Xiangxi

doi:10.1021/acs.jafc.0c07415

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…In our previous research, we discovered MLNs showed inhibitory activity against the germination of Sporisorium scitamineum [ 33 ]. Thus, a bacteriostatic circle test was performed to select the most robust mutant based on the bacteriostatic circle size (Fig.…”

Section: Resultsmentioning

confidence: 99%

Improvement of macrolactins production by the genetic adaptation of Bacillus siamensis A72 to saline stress via adaptive laboratory evolution

et al. 2022

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Background Macrolactins, a type of macrolide antibiotic, are toxic to the producer strains. As such, its level is usually maintained below the lethal concentration during the fermentation process. To improve the production of macrolactins, we applied adaptive laboratory evolution technology to engineer a saline-resistant mutant strain. The hypothesis that strains with saline resistance show improved macrolactins production was investigated. Results Using saline stress as a selective pressure, we engineered a mutant strain with saline resistance coupled with enhanced macrolactins production within 60 days using a self-made device. As compared with the parental strain, the evolved strain produced macrolactins with 11.93% improvement in non-saline stress fermentation medium containing 50 g/L glucose, when the glucose concentration increased to 70 g/L, the evolved strain produced macrolactins with 71.04% improvement. RNA sequencing and metabolomics results revealed that amino acid metabolism was involved in the production of macrolactins in the evolved strain. Furthermore, genome sequencing of the evolved strain revealed a candidate mutation, hisDD41Y, that was causal for the improved MLNs production, it was 3.42 times higher than the control in the overexpression hisDD41Y strain. Results revealed that saline resistance protected the producer strain from feedback inhibition of end-product (macrolide antibiotic), resulting in enhanced MLNs production. Conclusions In the present work, we successfully engineered a mutant strain with enhanced macrolactins production by adaptive laboratory evolution using saline stress as a selective pressure. Based on physiological, transcriptomic and genetic analysis, amino acid metabolism was found to benefit macrolactins production improvement. Our strategy might be applicable to improve the production of other kinds of macrolide antibiotics and other toxic compounds. The identification of the hisD mutation will allow for the deduction of metabolic engineering strategies in future research.

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Section: Resultsmentioning

confidence: 99%

Improvement of macrolactins production by the genetic adaptation of Bacillus siamensis A72 to saline stress via adaptive laboratory evolution

et al. 2022

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“…Although numerous macrolide compounds have been isolated and identified from sponges and their symbionts [ 108 ], this is the first study to report the isolation of macrolactins from sponge-associated bacteria. Macrolactin A ( 2 ) has been frequently isolated from different bacteria [ 30 , 31 , 32 , 109 , 110 , 111 , 112 ], whereas macrolactin H ( 3 ) and 15, 17-epoxy-16-hydroxy macrolactin A ( 4 ) have been rarely isolated [ 32 , 33 ]. Previous bioassays revealed that macrolactin A exhibited antimicrobial activity against Gram-negative bacteria (i.e., E. coli ), Gram-positive bacteria (i.e., S. aureus , methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), B. subtilis , E. faecalis, and vancomycin-resistant enterococci), as well as fungi (i.e., Botrytis cinerea, A. niger , B. cinerea , Colletotrichum acutatum , R. solani , S. cerevisiae , C. albicans, Pestalotiopsis theae , and C. gloeosporioides ) [ 30 , 31 , 32 , 109 , 110 , 111 , 112 ].…”

Section: Discussionmentioning

confidence: 99%

“…Macrolactin A ( 2 ) has been frequently isolated from different bacteria [ 30 , 31 , 32 , 109 , 110 , 111 , 112 ], whereas macrolactin H ( 3 ) and 15, 17-epoxy-16-hydroxy macrolactin A ( 4 ) have been rarely isolated [ 32 , 33 ]. Previous bioassays revealed that macrolactin A exhibited antimicrobial activity against Gram-negative bacteria (i.e., E. coli ), Gram-positive bacteria (i.e., S. aureus , methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), B. subtilis , E. faecalis, and vancomycin-resistant enterococci), as well as fungi (i.e., Botrytis cinerea, A. niger , B. cinerea , Colletotrichum acutatum , R. solani , S. cerevisiae , C. albicans, Pestalotiopsis theae , and C. gloeosporioides ) [ 30 , 31 , 32 , 109 , 110 , 111 , 112 ]. The antimicrobial assays in our studies showed similar results, in which macrolactin A exhibited antimicrobial activity against a broad spectrum of reference microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria

et al. 2021

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This study aimed to assess the diversity and antimicrobial activity of cultivable bacteria associated with Vietnamese sponges. In total, 460 bacterial isolates were obtained from 18 marine sponges. Of these, 58.3% belonged to Proteobacteria, 16.5% to Actinobacteria, 18.0% to Firmicutes, and 7.2% to Bacteroidetes. At the genus level, isolated strains belonged to 55 genera, of which several genera, such as Bacillus, Pseudovibrio, Ruegeria, Vibrio, and Streptomyces, were the most predominant. Culture media influenced the cultivable bacterial composition, whereas, from different sponge species, similar cultivable bacteria were recovered. Interestingly, there was little overlap of bacterial composition associated with sponges when the taxa isolated were compared to cultivation-independent data. Subsequent antimicrobial assays showed that 90 isolated strains exhibited antimicrobial activity against at least one of seven indicator microorganisms. From the culture broth of the isolated strain with the strongest activity (Bacillus sp. M1_CRV_171), four secondary metabolites were isolated and identified, including cyclo(L-Pro-L-Tyr) (1), macrolactin A (2), macrolactin H (3), and 15,17-epoxy-16-hydroxy macrolactin A (4). Of these, compounds 2-4 exhibited antimicrobial activity against a broad spectrum of reference microorganisms.

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“…Bamemacrolactin C was the most potent pathogen growth inhibitor and subsequent mechanism of action (MOA) studies indicated that this MNP targets the oxidative phosphorylation pathway in S. scitamineum. 10 Dermacozines N–P 22 , 23 , 24 , were isolated from a piezotolerant actinomycete strain Dermacoccus abyssi with dermacozine N 22 bearing a novel linear pentacyclic phenoxazine motif. 11 Genome mining of Fulvivirga sp.…”

Section: Marine Microorganisms and Phytoplanktonmentioning

confidence: 99%