Neutrophils, monocytes and other immune components in the equine endometrium: Friends or foes?

Skarzynski, Dariusz J.; Szóstek‐Mioduchowska, Anna; Rebordão, Maria Rosa; Jalali, Beenu Moza; Piotrowska-Tomala, K.K.; Leciejewska, Natalia; Łazarczyk, Miriam; Ferreira‐Dias, Graça

doi:10.1016/j.theriogenology.2020.01.018

Cited by 13 publications

(19 citation statements)

References 97 publications

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“…Research conducted regarding the type and severity of inflammation should give an insight into the role of this pathway in the regulation of modulatory cytokines. Differences in the regulation of this pathway may suggest separate mechanisms in the pathogenesis of endometrosis and endometritis and give another perspective on the influence of inflammation on the development of endometrosis, which has been widely studied (Aresu et al, 2012;Rebordão, Carneiro, et al, 2014;Rebordão, Galvão, et al, 2014;Klose & Schoon, 2016;Rebordão et al, 2018;Schöniger & Schoon, 2020;Skarzynski et al, 2020) An increase in the transcription of the MCP-1 gene in moderate and severe infiltration may suggest that its function is rather sustained in the inflammatory process, than its initiation. The highest transcription was found in macrophages-dominant infiltration.…”

Section: Genementioning

confidence: 99%

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The NF‐κB‐signalling pathway in mare's endometrium infiltrated with the inflammatory cells

Jasiński

Zdrojkowski

Kautz

et al. 2022

Reprod Domestic Animals

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As mucous membranes are a part of the integumentary system, they play a crucial role in the protection of the underlying tissues from damage and infection. Thus, it is reasonable that, even in healthy tissue, inflammatory cells are present. While some infiltrate cells are sparsely distributed along with the tissue, some can be found in lymphatic follicles and around the endometrial glands, such as mononuclear inflammatory cell infiltrates interpreted as a regular component of the mucosa-associated lymphoid tissue in submucous membranes (Klose & Schoon, 2016). This is the first barrier of an organism, while these cells are the first guardians against threats to homeostasis (Christoffersen & Troedsson, 2017). Mares' endometrium may be facing bacterial influx during oestrus, after

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Section: Genementioning

confidence: 99%

“…Endometritis and inflammation regulators are suspected to have a part in endometrosis pathogenesis, or even to be the main cause (Aresu et al, 2012;Rebordão, Carneiro, et al, 2014;Rebordão et al, 2018;Skarzynski et al, 2020).…”

mentioning

confidence: 99%

The NF‐κB‐signalling pathway in mare's endometrium infiltrated with the inflammatory cells

Jasiński

Zdrojkowski

Kautz

et al. 2022

Reprod Domestic Animals

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“…Estrogen regulates various types of cells in the endometrium, including epithelial cells, stromal cells, immune cells, and vascular cells. Therefore, the anabolic or regulatory imbalance of estrogen can cause pathological changes in the endometrium (Gibson et al 2015;Skarzynski et al 2020;Xu et al 2019;Yang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Up-regulation of DNA2 results in cell proliferation and migration in endometriosis

Wang

Zhang

et al. 2021

J Mol Histol

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Accumulating evidence has suggests that women with advanced endometriosis exhibit alterations in the expression of genes in the endometrium compared to healthy controls. Furthermore, replication stress is a characteristic feature of cancer cells, which results from sustained proliferative signaling induced by either the activation of oncogenes or the loss of tumor suppressors. In the present study, we propose that DNA replication ATP-dependent helicase/nuclease 2 (DNA2) might be upregulated in endometriosis. Immunohistochemical staining results confirmed the hypothesis that DNA2 is overexpressed in the eutopic/ectopic endometrium compared to that in a control endometrium from a healthy donor. Subsequently, ectopic endometrium-derived endometrial mesenchymal stem cells (EMSCs) showed the highest level of DNA2 and checkpoint kinase 1 (CHK1), as well as the strongest proliferation and migration capabilities, followed by eutopic endometrium-derived EMSCs, and then control EMSCs. To further analyze the function of DNA2, we knocked-down DNA2 expression in KLE cells. As expected, proliferation and migration declined when cells were transfected with DNA2 small interfering RNA. Taken together, our study demonstrated the overexpression of DNA2 in human endometriosis, which might be responsible for the upregulated cell proliferation and migration. This study provides insights into the mechanisms underlying human endometriosis.

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“…Jenny endometritis is characterized by the presence of large amounts of polymorphonuclear neutrophils (PMNs) and eosinophils migrating to the uterine lumen, as well as increased cytokine expression [2,3]. After infection, neutrophils destroy pathogens by phagocytosis and by degranulation, producing DNA complexes that combine with nuclear and cytoplasmic proteins to form web-like extracellular structures called neutrophil extracellular traps (NETs) [4]. However, after intrauterine semen deposition, the PMN endometrial infiltration is induced by spermatozoa rather than bacteria [5].…”

Section: Introductionmentioning

confidence: 99%

“…NETs persistence has also been associated with the development of progressive fibrosis. Prostaglandins, inflammatory cytokines, and proteases found in NETs (e.g., elastase (ELA), cathepsin G (CAT), and myeloperoxidase (MPO)) act as pro-fibrotic factors in equine endometrial fibrosis by inducing collagen type I (COL1) accumulation [4,[8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis

Fantini

Jiménez

Vilés

et al. 2021

Animals

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The aim of this study was to standardize a simple, manual platelet-rich plasma (PRP) protocol in Catalonian donkeys using single-spin tube centrifugation as a treatment for jenny endometritis. The objective was to obtain a blood product with a moderate concentration of platelets (2 or 3 times baseline physiologic values) and a low WBC (White Blood Cells) concentration. Blood was drawn from six Catalonian donkeys using acid citrate dextrose (ACD) as an anticoagulant, and then processed by single centrifugation at 133× g for two different centrifugation times (10 and 15 min). The PRP samples were evaluated by flow cytometry, and TGF-β1 (Transforming Growth Factor-Beta1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The 10 min centrifugation protocol resulted in a slightly greater release of TGF-β1 (6044.79 ng/mL), a 2.06-fold increase in platelet concentration, and a 15-fold reduction in leukocyte concentration when compared to the initial values. The 15 min centrifugation time resulted in a 2.44-fold increase in baseline platelet concentration, a reduction in WBC count by a factor of 20, and slightly lower TGF levels (5206 ng/mL). We conclude that both protocols are adequate for the obtention of PRP, and both may have an acceptable therapeutic potential for use in this species, although this needs to be further validated.

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Neutrophils, monocytes and other immune components in the equine endometrium: Friends or foes?

Cited by 13 publications

References 97 publications

The NF‐κB‐signalling pathway in mare's endometrium infiltrated with the inflammatory cells

The NF‐κB‐signalling pathway in mare's endometrium infiltrated with the inflammatory cells

Up-regulation of DNA2 results in cell proliferation and migration in endometriosis

Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis

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