Neutrophils, lymphocytes, and monocytes exhibit diverse behaviors in transendothelial and subendothelial migrations under coculture with smooth muscle cells in disturbed flow

Chen, Cheng Nan; Chang, Shang‐Tzen; Lee, Pei Ling; Chang, Kyle; Chen, Li Jing; Usami, Shunichi; Chien, Shu; Chiu, Jeng Jiann

doi:10.1182/blood-2005-08-3137

Cited by 69 publications

(59 citation statements)

References 48 publications

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“…At low shear stress (1.25 dyne/cm 2 ) at 1 hr, the recirculation zone contained the most adherent NB4 cells in the presence of a TNF-α stimulated endothelium, Figure 7. Unlike Chen et al we did not find leukocyte clustering at the reattachment point (13). This may be due to a well documented instability of the reattachment point, creating high temporal shear stress gradients (50).…”

Section: Discussioncontrasting

confidence: 91%

“…Under their flow conditions, attachment was only found in the recirculation zone and none was seen in the recovery region distal to the reattachment point (29). In the same reverse step model, Chen et al showed that neutrophils, lymphocytes and monocytes adhered and transmigrated more in the reattachment region than in the recirculation and did not note any real difference between the recirculation and recovery (distal) regional attachment (13). They hypothesize a longer residence time and a higher near wall concentration as cells roll more slowly in the reattachment point compared to other areas possibly causing this preferential attachment.…”

Section: Discussionmentioning

confidence: 97%

“…In vitro studies have confirmed the ability of ECs to alter their phenotype in response to fluid shear stress into a pro-inflammatory or proatherogenic phenotype (10,11). The recruitment and attachment of leukocyte to the endothelium is dependent on the cell phenotype and the local fluid forces acting on the circulating leukocyte (12,13). The local response of the endothelial cells and the complex hemodynamics created by the geometry of a stenosis likely plays a role in the regional attachment of leukocytes.…”

Section: Introductionmentioning

confidence: 90%

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Neutrophil Adhesion on Endothelial Cells in a Novel Asymmetric Stenosis Model: Effect of Wall Shear Stress Gradients

et al. 2010

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Leukocytes play a pivotal role in the progression of atherosclerosis. A novel three dimensional in vitro asymmetric stenosis model was used to better investigate the role of local hemodynamics in the adhesion of leukocytes to an established plaque. The adhesion of a human promyelocytic cell line (NB4) on a human abdominal aortic endothelial cell (EC) monolayer was quantified. NB4 cells were circulated over TNF-α stimulated and non-stimulated ECs for 1 or 6 hrs at 1.25 or 6.25 dynes/cm 2 and compared to static conditions. Cytokine stimulation increased significantly endothelial cell expression of intercellular adhesion molecule and vascular cell adhesion molecule. Under static conditions, neutrophils adhered overall more than under flow, with decreased adhesion with increasing shear. Adhesion was significantly higher in the recirculation region distal to the stenosis than in the inlet. Preshearing the endothelial cells decreased the expression of cell adhesion molecules in inflamed endothelium and significantly decreased adhesion. However, the ratio of adhesion between the recirculation zone and the inlet increased, hence exhibiting an increased regional difference. This work suggests an important role for neutrophil-endothelial cell interactions in the atherosclerotic process, especially in wall shear gradient regions. This is important clinically, potentially helping to explain plaque stability.

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Section: Discussioncontrasting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 90%

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Neutrophil Adhesion on Endothelial Cells in a Novel Asymmetric Stenosis Model: Effect of Wall Shear Stress Gradients

et al. 2010

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“…75 There was significant adhesion of neutrophils, lymphocytes, and monocytes to IL-1b activated co-culture system consisting of ECs seed on SMCs in the reattachment zone of this model. Co-culturing ECs with SMCs significantly amplified the adhesion of leukocytes relative to ECs cultured alone.…”

Section: Flow-based In Vitro Models and Cams Expressionmentioning

confidence: 72%

Targeting cell adhesion molecules with nanoparticles using in vivo and flow-based in vitro models of atherosclerosis

Khodabandehlou

Masehi-Lano

Poon

et al. 2017

Exp Biol Med (Maywood)

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Impact statementAs atherosclerosis remains the leading cause of death, there is an urgent need to develop better tools for treatment of the disease. The ability to improve current treatments relies on enhancing the accuracy of in vitro and in vivo atherosclerotic models. While in vivo models provide all the relevant testing parameters, variability between animals and among models used is a barrier to reproducible results and comparability of NP efficacy. In vitro cultures isolate cells into microenvironments that fail to take into account flow separation and shear stress, which are characteristics of atherosclerotic lesions. Flow-based in vitro models provide more physiologically relevant platforms, bridging the gap between in vivo and 2D in vitro models. This is the first review that presents recent advances regarding endothelial cell-targeting using adhesion molecules in light of in vivo and flow-based in vitro models, providing insights for future development of optimal strategies against atherosclerosis. AbstractAtherosclerosis is a leading cause of death worldwide; in addition to lipid dysfunction, chronic arterial wall inflammation is a key component of atherosclerosis. Techniques that target cell adhesion molecules, which are overexpressed during inflammation, are effective methods to detect and treat atherosclerosis. Specifically, research groups have identified vascular cell adhesion molecule-1, intercellular adhesion molecule-1, platelet endothelial cell adhesion molecule, and selectins (E-selectin and P-selectin) as correlated to atherogenesis. In this review, we discuss recent strategies both in vivo and in vitro that target cell adhesion molecules. First, we discuss peptide-based and antibody (Ab)-based nanoparticles utilized in vivo for diagnostic, therapeutic, and theranostic applications. Second, we discuss flow-based in vitro models that serve to reduce the traditional disadvantages of in vivo studies such as variability, time to develop the disease, and ethical burden, but preserve physiological relevance. The knowledge gained from these targeting studies can be translated into clinical solutions for improved detection, prevention, and treatment of atherosclerosis.

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“…Also, leukocyte adhesion and transendothelial migration are increased with activated ECs, which can be attributed to increased intercellular adhesion molecule-1, Tie1, and E-selectin expression. 66,68 …”

Section: Disturbed Venous Blood Flow and Inflammation And Thrombosismentioning

confidence: 99%

Coagulation and the Vessel Wall in Pulmonary Embolism

Alias

Lang

2013

Pulm. circ.

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Venous thromboembolism comprises deep-vein thrombosis, thrombus in transit, acute pulmonary embolism, and chronic thromboembolic pulmonary hypertension (CTEPH). Pulmonary thromboemboli commonly resolve, with restoration of normal pulmonary hemodynamics. When they fail to resorb, permanent occlusion of the deep veins and/or CTEPH are the consequences. Apart from endogenous fibrinolysis, venous thrombi resolve by a process of mechanical fragmentation, through organization of the thromboembolus by invasion of endothelial cells, leukocytes, and fibroblasts leading to recanalization. Recent data utilizing various models have contributed to a better understanding of venous thrombosis and the resolution process that is directed at maintaining vascular patency. This review summarizes the plasmatic and cellular components of venous thrombus formation and resolution.

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Neutrophils, lymphocytes, and monocytes exhibit diverse behaviors in transendothelial and subendothelial migrations under coculture with smooth muscle cells in disturbed flow

Cited by 69 publications

References 48 publications

Neutrophil Adhesion on Endothelial Cells in a Novel Asymmetric Stenosis Model: Effect of Wall Shear Stress Gradients

Neutrophil Adhesion on Endothelial Cells in a Novel Asymmetric Stenosis Model: Effect of Wall Shear Stress Gradients

Targeting cell adhesion molecules with nanoparticles using in vivo and flow-based in vitro models of atherosclerosis

Coagulation and the Vessel Wall in Pulmonary Embolism

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