Neutrophils Induce the Maturation of Immature Dendritic Cells: A Regulatory Role of Neutrophils in Adaptive Immune Responses

Xiaoxiao, Wan; Yue, Sibiao; Xiong, Xiaoli; Zheng, Pan; Chen, Gang

doi:10.1080/08820130601109719

Cited by 10 publications

(8 citation statements)

References 20 publications

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“…A recent report by Yang et al [43] described the negative influence of neutrophils on the T and B cell responses to protein antigens administered by needle in the footpad. It is clear, however, that apoptotic neutrophils can also provide a source of immunogenic molecules to DC, especially for cross-priming, and especially if accompanied by extrinsic maturation signals [44], [45]. The relative paucity of activation signals associated with the phagocytosis of Leishmania promastigotes by neutrophils is suggested by the fact that the parasite traffics to a non-lytic compartment, avoids activation of the NADPH oxidase, and survives capture by these cells [5], [37].…”

Section: Discussionmentioning

confidence: 99%

Efficient Capture of Infected Neutrophils by Dendritic Cells in the Skin Inhibits the Early Anti-Leishmania Response

et al. 2012

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Neutrophils and dendritic cells (DCs) converge at localized sites of acute inflammation in the skin following pathogen deposition by the bites of arthropod vectors or by needle injection. Prior studies in mice have shown that neutrophils are the predominant recruited and infected cells during the earliest stage of Leishmania major infection in the skin, and that neutrophil depletion promotes host resistance to sand fly transmitted infection. How the massive influx of neutrophils aimed at wound repair and sterilization might modulate the function of DCs in the skin has not been previously addressed. The infected neutrophils recovered from the skin expressed elevated apoptotic markers compared to uninfected neutrophils, and were preferentially captured by dermal DCs when injected back into the mouse ear dermis. Following challenge with L. major directly, the majority of the infected DCs recovered from the skin at 24 hr stained positive for neutrophil markers, indicating that they acquired their parasites via uptake of infected neutrophils. When infected, dermal DCs were recovered from neutrophil depleted mice, their expression of activation markers was markedly enhanced, as was their capacity to present Leishmania antigens ex vivo. Neutrophil depletion also enhanced the priming of L. major specific CD4+ T cells in vivo. The findings suggest that following their rapid uptake by neutrophils in the skin, L. major exploits the immunosuppressive effects associated with the apoptotic cell clearance function of DCs to inhibit the development of acquired resistance until the acute neutrophilic response is resolved.

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Section: Discussionmentioning

confidence: 99%

Efficient Capture of Infected Neutrophils by Dendritic Cells in the Skin Inhibits the Early Anti-Leishmania Response

et al. 2012

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“…Neutrophils have the ability to sense bacteria and their components and to migrate across epithelia in response to chemotactic gradients generated from the site of infection (31,32). Although neutrophils are best known for their phagocytic and bactericidal capacity, it is becoming increasingly evident that they also play an active role in assisting other immune cells to mount an effective adaptive immune response (33,34).…”

Section: Discussionmentioning

confidence: 99%

LL-37 Promotes Rapid Sensing of CpG Oligodeoxynucleotides by B Lymphocytes and Plasmacytoid Dendritic Cells

Hurtado

Peh

2009

The Journal of Immunology

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LL-37 is a cationic antimicrobial peptide derived from neutrophils and keratinocytes. It plays an important role in protection against bacterial infection in the skin and mucosal surfaces. However, its role within the blood compartment remains unclear given that serum inhibits its bactericidal property. In this study, we show that LL-37 promotes very rapid and highly efficient sensing of CpG motifs in bacterial DNA by human B lymphocytes and plasmacytoid dendritic cells (pDCs) in serum-containing media and in whole blood. LL-37 allowed detection of CpG oligodeoxynucleotide (ODN) within minutes of exposure. Without LL-37, 20–30 times more CpG was required to produce the same effect. The promotion of CpG detection by LL-37 was independent of the backbone of the ODN, as the effect was observed not only in ODNs with modified phosphorothioate backbone, but also in ODNs with natural phosphodiester backbone, as found in genomic DNA. Unmethylated CpG motifs within the phosphodiester ODN and LL-37–mediated delivery are required for pDCs to respond. In keeping with the above, cells responded to CpG-rich bacterial DNA and LL-37, but not to human DNA and LL-37. The ability of LL-37 to enhance delivery of CpG to stimulate immune cells is independent of its amphipathic structure and its bactericidal property. LL-37 aids the delivery of CpG to B cells and pDCs, but not T cells. These findings are pertinent to rapid recognition of microbial DNA and are highly relevant to contemporary studies of CpG/TLR9 agonists in vaccines and cancer therapy.

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“…In both human (49) and mouse models (45,51), the local production of TNF-α by neutrophils physically associated or close to DCs is required to induce DC activation.…”

Section: Molecular Mechanisms Of Neutrophil-dc Communicationmentioning

confidence: 99%

Activation of dendritic cells by polymorphonuclear neutrophils

Boudaly

2009

Front Biosci

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Neutrophils Induce the Maturation of Immature Dendritic Cells: A Regulatory Role of Neutrophils in Adaptive Immune Responses

Cited by 10 publications

References 20 publications

Efficient Capture of Infected Neutrophils by Dendritic Cells in the Skin Inhibits the Early Anti-Leishmania Response

Efficient Capture of Infected Neutrophils by Dendritic Cells in the Skin Inhibits the Early Anti-Leishmania Response

LL-37 Promotes Rapid Sensing of CpG Oligodeoxynucleotides by B Lymphocytes and Plasmacytoid Dendritic Cells

Activation of dendritic cells by polymorphonuclear neutrophils

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