Neutrophils from patients with the cardiac clinical form of Chagas disease release less NETs than neutrophils from healthy individuals

Almeida, Valéria Duarte de; Câmara, None Eduardo B.L. da; Castro, Marcelo; Pimenta, Suyane Maria P; Hernandes, None Bruna Luiza T.; Paiva, Dayane Carla Costa; Andrade, Cléber de Mesquita; Nascimento, Ellany Gurgel Cosme do; Fernandes, Thales Allyrio A M; Andrade, Micássio Fernandes de

doi:10.1111/pim.12821

Cited by 3 publications

References 30 publications

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Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Kolesnichenko,

Kazmina,

Chistov

et al. 2023

Amino Acids

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Polystyrene-based granulated support Bio-Beads® SM-2 was employed for the separation of peptide-pnitroanilides from Na 2 SO 4 and Na 2 SO 5 mixture (Oxone®) after oxidation of peptide-p-aminoanilides.Unsubstituted phenyl-containing N-terminal protection groups (such as carbobenzoxy or tested earlier benzoyl) and Phe residues ensured an e cient adsorption of peptide-p-nitroanilides onto Bio-Beads® SM-2. Neither such aromatic groups as tosyl, 9-uorenyl(methoxycarbonyl), p-nitroanilide nor indolyl or phydroxyphenyl groups of Trp and Tyr residues provide peptide-p-nitroanilides with the ability to adsorb onto the polystyrene-based support. Hence, peptides and their derivatives should contain at least one unsubstituted phenyl group to ensure an e cient separation and puri cation via adsorption chromatography on Bio-Beads® SM-2. This chromatographic support is well suitable for desalting or separation of phenyl group-containing peptides, especially in multiple parallel preparation and highthroughput screening experiments.

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Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Kolesnichenko,

Kazmina,

Chistov

et al. 2023

Amino Acids

View full text Add to dashboard Cite

show abstract

Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Kolesnichenko,

Kazmina,

Chistov

et al. 2023

Preprint

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Polystyrene-based granulated support Bio-Beads® SM-2 was employed for the separation of peptide-p-nitroanilides from Na2SO4 and Na2SO5 mixture (Oxone®) after oxidation of peptide-p-aminoanilides. Unsubstituted phenyl-containing N-terminal protection groups (such as carbobenzoxy or tested earlier benzoyl) and Phe residues ensured an efficient adsorption of peptide-p-nitroanilides onto Bio-Beads® SM-2. Neither such aromatic groups as tosyl, 9-fluorenyl(methoxycarbonyl), p-nitroanilide nor indolyl or p-hydroxyphenyl groups of Trp and Tyr residues provide peptide-p-nitroanilides with the ability to adsorb onto the polystyrene-based support. Hence, peptides and their derivatives should contain at least one unsubstituted phenyl group to ensure an efficient separation and purification via adsorption chromatography on Bio-Beads® SM-2. This chromatographic support is well suitable for desalting or separation of phenyl group-containing peptides, especially in multiple parallel preparation and high-throughput screening experiments.

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Intratumoral neutrophil extracellular traps are associated with unfavorable clinical outcomes and immunogenic context in pancreatic ductal adenocarcinoma

Chen

et al. 2022

Front. Immunol.

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Extracellular traps (ETs) and tumor-infiltrating immune cells play crucial roles in tumor progression. However, little is known about the clinical significance of tumor-infiltrating neutrophils and macrophages and the related ETs in pancreatic ductal adenocarcinoma (PDAC). This study investigates the associations between neutrophil or macrophage infiltration or ET formation and the clinicopathological features, molecular characteristics, immune checkpoint molecules, clinical outcomes, and response to adjuvant chemotherapy (ACT) in PDAC. We performed multiplex immunofluorescence staining to detect ET formation by neutrophils or macrophages using tissue microarrays obtained from 205 patients, and analyzed the immunohistochemistry data for PD-L1, PD-L2, B7-H3, and B7-H4. The ET expression rates in macrophages and neutrophils were 23.9% and 45.4%, respectively. Patients with a high density of neutrophils or positive expression of neutrophil ETs exhibited poorer progression-free survival (PFS) and disease-specific survival (DSS), whereas macrophage ETs were not related to PFS and DSS. Neutrophil infiltration and ET formation were identified as independent prognostic predictors of DSS using univariate and multivariate Cox analyses. Patients with PDAC with lower neutrophil infiltration or negative staining for neutrophil ETs are more likely to benefit from ACT. Patients with PDAC were more accurately stratified based on the infiltration of neutrophils and presence of neutrophil ETs, and patients with low neutrophil infiltration and negative staining for neutrophil ETs showed the best survival. Patients with positive neutrophil ETs demonstrated inferior DSS compared to those with negative neutrophil ETs in the PD-L1 tumor proportion score (TPS) < 1% and PD-L1 IC < 1% subgroups. However, the positive expression of neutrophil ETs was not related to DSS in the PD-L1 TPS ≥ 1% or PD-L1 IC ≥ 1% subgroup. Our findings emphasize the potential of neutrophil infiltration and ETs as prognostic markers that could guide the formulation of more effective personalized treatments for PDAC.

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Neutrophils from patients with the cardiac clinical form of Chagas disease release less NETs than neutrophils from healthy individuals

Cited by 3 publications

References 30 publications

Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Influence of amino acid and N-terminal protection residue structures on peptide p-nitroanilide adsorption on polystyrene-based support

Intratumoral neutrophil extracellular traps are associated with unfavorable clinical outcomes and immunogenic context in pancreatic ductal adenocarcinoma

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