Neutrophils exert protection in early Aeromonas veronii infections through the clearance of both bacteria and dying macrophages

Havixbeck, Jeffrey J.; Rieger, Aja M.; Churchill, Lucas; Barreda, Daniel R.

doi:10.1016/j.fsi.2017.02.001

Cited by 32 publications

(26 citation statements)

References 60 publications

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“…Aeromonas veronii, a gram-negative opportunistic pathogen, is associated with infections in both fish and mammals [1,2]. A. veronii is widely distributed in fresh water, sludge, sewage, soil and other natural environments [3].…”

Section: Introductionmentioning

confidence: 99%

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Tian

Kang

Chu

et al. 2019

IJMS

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Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune responses in fish administered with recombinant L. casei were evaluated. The two recombinant L. casei were orally administered to common carp, which stimulated high serum IgM and induced higher ACP, AKP, SOD and LYZ activity. Using qRT-PCR, the expression of IL-10, IL-8, IL-1β, TNF-α and IFN-γ in the tissue of fish immunized with recombinant L. casei was significantly (p < 0.05) upregulated, which indicated that recombinant L. casei could activate the innate immune system to trigger the cell immune response and inflammatory response. Furthermore, recombinant L. casei was able to survive the intestinal environment and colonize in intestine mucosal. The study showed that after being challenged by A. veronii, fish administered with Lc-pPG-1-FlaA (70%) and Lc-pPG-2-FlaA (50%) had higher survival rates compared to Lc-pPG and PBS, indicating that recombinant L. casei might prevent A. veronii infection by activating the immune system to trigger immune responses. We demonstrated that flagellin as an antigen of vaccine, is acceptable for preventing A. veronii infection in fish. The recombinant L. casei expressing FlaA may be a novel mucosal vaccine for treating and controlling A. veronii.

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Section: Introductionmentioning

confidence: 99%

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Tian

Kang

Chu

et al. 2019

IJMS

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“…Aeromonas veronii is a common aquatic pathogen, which has been reported to cause the death of many aquatic animals (Havixbeck et al, 2017; Jiang et al, 2019). In the present study, C. argus fed with thymol‐supplement diets, resulting in the enhancement of survival rate against A. veronii TH0426.…”

Section: Discussionmentioning

confidence: 99%

The optimum thymol requirement in diets of Channa argus : effects on growth, antioxidant capability, immune response and disease resistance

Kong

Xia

et al. 2021

Aquacult. Nutr.

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In the present study, we aimed to evaluate the effects of dietary thymol on growth, antioxidant status, liver health, immune response and disease resistance of snakehead fish (Channa argus) (initial weight, 11.86 ± 0.15 g). The fish were fed for 56 days with a basal diet supplemented with thymol at 0, 150, 300, 450, 600 and 750 mg/kg, leading to six experimental diets. The final body weight (FBW), weight gain (WG), protein efficiency ratio (PER) and specific growth rate (SGR) were increased as dietary thymol level increased, arriving a summit value at 450 mg/kg dietary thymol level, and then, values of these indices started to decrease when dietary thymol level further rose, and feed conversion ratio (FCR) is the opposite state. Our results showed a significant increase (p < .05) in the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‐Px) activities in liver, spleen, head kidney and intestine with diets supplemented with 300 and 450 mg/kg thymol compared to the control and significantly decrease (p < .05) in the malondialdehyde (MDA) activity. Results showed that dietary supplementation with 300 and 450 mg/kg thymol significantly increased (p < .05) the serum acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM) activities and immunoglobulin M (IgM), complement 3 (C3), complement 4 (C4) concentrations of C. argus compared to the control, and decreased (p < .05) the serum glutamic‐pyruvic transaminase (ALT) and glutamic‐oxaloacetic transaminase (AST) activities. Moreover, the expression of HSP70, TNF‐α, IL‐1β and IL‐8 were down‐regulated in liver, head kidney and intestine by dietary supplementation with 300 and 450 mg/kg thymol, and the expression of IL‐10 and TGF‐β was up‐regulated. After challenge with Aeromonas veronii, survival rates in all thymol treatment groups were significantly increased (p < .05). Consequently, our results indicate that adequate dietary supplementation thymol can effectively enhance the growth, antioxidant status, immune response and disease resistance of C. argus, and the optimum dietary thymol requirement for maximum WG of C. argus was estimated to be 356 mg/kg.

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“…Aeromonas veronii is a Gram-negative pathogenic bacterium that is fatal to naturally infected fish and is a substantial burden on the aquaculture industry. In the establishment of an infected goldfish model, neutrophils were found to play a role in pathogen defence against early A. veronii infection by eliminating bacteria and pyroptotic macrophages (Havixbeck et al 2017). Compared with our understanding of bacterial diseases, little is known about pyroptosis induced by viral infection.…”

Section: Pyroptosis In Fishmentioning

confidence: 99%

Lytic regulated cell death in aquaculture fish

Xia

Zhang

et al. 2021

Reviews in Aquaculture

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Cell death is an important physiological and pathological phenomenon in multicellular animals and occurs widely in various body's systems. In recent years, researchers have gained a deeper understanding of the mechanisms of necrosis and apoptosis in mammals. In addition, many new forms of lytic regulated cell death (RCD), such as pyroptosis, necroptosis and ferroptosis, have been discovered, thus enhancing our understanding of the molecular mechanisms of programmed cell death (PCD) and the associated signalling pathways as well as their relationship with diseases. However, our understanding of lytic RCD in fish is limited. Recently, some progress has been made regarding the induction conditions, expression of related genes and regulation of lytic RCD in fish. Furthermore, research on the roles of lytic RCD in tissue development, against pathogenic microorganism, inflammatory disease and neurological disease, has also been advanced. Understanding lytic RCD in fish will enable the regulation of its occurrence through human intervention to inhibit disease processes, improve fish health and explore possible countermeasures based on these basic theoretical studies.

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Neutrophils exert protection in early Aeromonas veronii infections through the clearance of both bacteria and dying macrophages

Cited by 32 publications

References 60 publications

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

The optimum thymol requirement in diets of Channa argus : effects on growth, antioxidant capability, immune response and disease resistance

Lytic regulated cell death in aquaculture fish

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