Neutrophilic lithotrophic iron-oxidizing prokaryotes and their role in the biogeochemical processes of the iron cycle

Дубинина, Г. А.; Sorokina, A. Yu.

doi:10.1134/s0026261714020052

Cited by 32 publications

(19 citation statements)

References 80 publications

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“…The cells were photographed using an Olympus BX 41 microscope. Ferric iron salts were dissolved with 1% oxalic acid under visual control in order to reveal morphology of the cells precipitating minerals (Dubinina and Sorokina, 2014). For ultrathin sectioning, the material was treated as described previously (Lunina et al, 2013).…”

Section: Study Area Sample Collection and Characterizationmentioning

confidence: 99%

Microbial processes of the carbon and sulfur cycles in an ice‐covered, iron‐rich meromictic lake Svetloe (Arkhangelsk region, Russia)

Savvichev

Kokryatskaya

Zabelina

et al. 2016

Environmental Microbiology

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Biogeochemical, isotope geochemical and microbiological investigation of Lake Svetloe (White Sea basin), a meromictic freshwater was carried out in April 2014, when ice thickness was ∼0.5 m, and the ice-covered water column contained oxygen to 23 m depth. Below, the anoxic water column contained ferrous iron (up to 240 μμM), manganese (60 μM), sulfide (up to 2 μM) and dissolved methane (960 μM). The highest abundance of microbial cells revealed by epifluorescence microscopy was found in the chemocline (redox zone) at 23-24.5 m. Oxygenic photosynthesis exhibited two peaks: the major one (0.43 μmol C L day ) below the ice and the minor one in the chemocline zone, where cyanobacteria related to Synechococcus rubescens were detected. The maximum of anoxygenic photosynthesis (0.69 μmol C L day ) at the oxic/anoxic interface, for which green sulfur bacteria Chlorobium phaeoclathratiforme were probably responsible, exceeded the value for oxygenic photosynthesis. Bacterial sulfate reduction peaked (1.5 μmol S L day ) below the chemocline zone. The rates of methane oxidation were as high as 1.8 μmol CH L day at the oxi/anoxic interface and much lower in the oxic zone. Small phycoerythrin-containing Synechococcus-related cyanobacteria were probably involved in accumulation of metal oxides in the redox zone.

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Section: Study Area Sample Collection and Characterizationmentioning

confidence: 99%

Microbial processes of the carbon and sulfur cycles in an ice‐covered, iron‐rich meromictic lake Svetloe (Arkhangelsk region, Russia)

Savvichev

Kokryatskaya

Zabelina

et al. 2016

Environmental Microbiology

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“…Using Fe(II) as their energy source, FeOB grow in acidic to neutral and oxic to anoxic waters (Konhauser, 1998;Fortin and Langley, 2005;Kappler and Straub, 2005;Hedrich et al, 2011;Konhauser et al, 2011;Templeton, 2011;Dubinina and Sorokina, 2014). FeOB cells, excreting extracellular polymeric substances, are often rapidly entombed by Fe-oxyhydroxides due to metabolic activities (Kappler and Newman, 2004;Chan et al, 2009Chan et al, , 2011Miot et al, 2009;Schädler et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Biogenic Iron-Rich Filaments in the Quartz Veins in the Uppermost Ediacaran Qigebulake Formation, Aksu Area, Northwestern Tarim Basin, China: Implications for Iron Oxidizers in Subseafloor Hydrothermal Systems

et al. 2015

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Fe-(oxyhydr)oxide-encrusted filamentous microstructures produced by microorganisms have been widely reported in various modern and ancient extreme environments; however, the iron-dependent microorganisms preserved in hydrothermal quartz veins have not been explored in detail because of limited materials available. In this study, abundant well-preserved filamentous microstructures were observed in the hydrothermal quartz veins of the uppermost dolostones of the terminal-Ediacaran Qigebulake Formation in the Aksu area, northwestern Tarim Basin, China. These filamentous microstructures were permineralized by goethite and hematite as revealed by Raman spectroscopy and completely entombed in chalcedony and quartz cements. Microscopically, they are characterized by biogenic filamentous morphologies (commonly 20-200 μm in length and 1-5 μm in diameter) and structures (curved, tubular sheath-like, segmented, and mat-like filaments), similar to the Fe-oxidizing bacteria (FeOB) living in modern and ancient hydrothermal vent fields. A previous study revealed that quartz-barite vein swarms were subseafloor channels of low-temperature, silica-rich, diffusive hydrothermal vents in the earliest Cambrian, which contributed silica to the deposition of the overlying bedded chert of the Yurtus Formation. In this context, this study suggests that the putative filamentous FeOB preserved in the quartz veins might have thrived in the low-temperature, silica- and Fe(II)-rich hydrothermal vent channels in subseafloor mixing zones and were rapidly fossilized by subsequent higher-temperature, silica-rich hydrothermal fluids in response to waning and waxing fluctuations of diffuse hydrothermal venting. In view of the occurrence in a relatively stable passive continental margin shelf environment in Tarim Block, the silica-rich submarine hydrothermal vent system may represent a new and important geological niche favorable for FeOB colonization, which is different from their traditional habitats reported in hydrothermal vent systems at oceanic spreading centers or volcanic seamounts. Thus, these newly recognized microfossils offer a new clue to explore the biological signatures and habitat diversity of microorganisms on Earth and beyond.

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“…KEYWORDS nitrate-dependent Fe(II) oxidation, cell-mineral aggregates, green rust N itrate-reducing Fe(II)-oxidizing bacteria (NRFeOB) have been found in soils, freshwater, brackish water, and marine environments (1)(2)(3)(4)(5)(6)(7)(8)(9). They link the nitrogen, carbon, and Fe cycles and potentially play a major role in Fe cycling.…”

mentioning

confidence: 99%

“…They link the nitrogen, carbon, and Fe cycles and potentially play a major role in Fe cycling. The products of microbial Fe(II) oxidation coupled to nitrate reduction are Fe(III) minerals and dinitrogen or intermediates of the denitrification pathway, e.g., NO 2 Ϫ , NO, and N 2 O (10-12). Most described NRFeOB require an organic cosubstrate to allow for their continuous cultivation and continuous oxidation of Fe(II) to Fe(III) (1,2,6,(9)(10)(11)(13)(14)(15)(16)(17)(18)(19)(20).…”

mentioning

confidence: 99%

Insights into Nitrate-Reducing Fe(II) Oxidation Mechanisms through Analysis of Cell-Mineral Associations, Cell Encrustation, and Mineralogy in the Chemolithoautotrophic Enrichment Culture KS

Nordhoff

Tominski

Halama

et al. 2017

Appl Environ Microbiol

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Most described nitrate-reducing Fe(II)-oxidizing bacteria (NRFeOB) are mixotrophic and depend on organic cosubstrates for growth. Encrustation of cells in Fe(III) minerals has been observed for mixotrophic NRFeOB but not for autotrophic phototrophic and microaerophilic Fe(II) oxidizers. So far, little is known about cellmineral associations in the few existing autotrophic NRFeOB. Here, we investigate whether the designated autotrophic Fe(II)-oxidizing strain (closely related to Gallionella and Sideroxydans) or the heterotrophic nitrate reducers that are present in the autotrophic nitrate-reducing Fe(II)-oxidizing enrichment culture KS form mineral crusts during Fe(II) oxidation under autotrophic and mixotrophic conditions. In the mixed culture, we found no significant encrustation of any of the cells both during autotrophic oxidation of 8 to 10 mM Fe(II) coupled to nitrate reduction and during cultivation under mixotrophic conditions with 8 to 10 mM Fe(II), 5 mM acetate, and 4 mM nitrate, where higher numbers of heterotrophic nitrate reducers were present. Two pure cultures of heterotrophic nitrate reducers (Nocardioides and Rhodanobacter) isolated from culture KS were analyzed under mixotrophic growth conditions. We found green rust formation, no cell encrustation, and only a few mineral particles on some cell surfaces with 5 mM Fe(II) and some encrustation with 10 mM Fe(II). Our findings suggest that enzymatic, autotrophic Fe(II) oxidation coupled to nitrate reduction forms poorly crystalline Fe(III) oxyhydroxides and proceeds without cellular encrustation while indirect Fe(II) oxidation via heterotrophic nitrate-reductionderived nitrite can lead to green rust as an intermediate mineral and significant cell encrustation. The extent of encrustation caused by indirect Fe(II) oxidation by reactive nitrogen species depends on Fe(II) concentrations and is probably negligible under environmental conditions in most habitats.IMPORTANCE Most described nitrate-reducing Fe(II)-oxidizing bacteria (NRFeOB) are mixotrophic (their growth depends on organic cosubstrates) and can become encrusted in Fe(III) minerals. Encrustation is expected to be harmful and poses a threat to cells if it also occurs under environmentally relevant conditions. Nitrite produced during heterotrophic denitrification reacts with Fe(II) abiotically and is probably the reason for encrustation in mixotrophic NRFeOB. Little is known about cell-mineral associations in autotrophic NRFeOB such as the enrichment culture KS. Here, we show that no encrustation occurs in culture KS under autotrophic and mixotrophic conditions while heterotrophic nitrate-reducing isolates from culture KS become en-

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Neutrophilic lithotrophic iron-oxidizing prokaryotes and their role in the biogeochemical processes of the iron cycle

Cited by 32 publications

References 80 publications

Microbial processes of the carbon and sulfur cycles in an ice‐covered, iron‐rich meromictic lake Svetloe (Arkhangelsk region, Russia)

Microbial processes of the carbon and sulfur cycles in an ice‐covered, iron‐rich meromictic lake Svetloe (Arkhangelsk region, Russia)

Biogenic Iron-Rich Filaments in the Quartz Veins in the Uppermost Ediacaran Qigebulake Formation, Aksu Area, Northwestern Tarim Basin, China: Implications for Iron Oxidizers in Subseafloor Hydrothermal Systems

Insights into Nitrate-Reducing Fe(II) Oxidation Mechanisms through Analysis of Cell-Mineral Associations, Cell Encrustation, and Mineralogy in the Chemolithoautotrophic Enrichment Culture KS

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