Neutrophil trafficking on-a-chip: an <i>in vitro</i>, organotypic model for investigating neutrophil priming, extravasation, and migration with spatiotemporal control

McMinn, Patrick H.; Hind, Laurel E.; Huttenlocher, Anna; Beebe, David J.

doi:10.1039/c9lc00562e

Cited by 30 publications

(30 citation statements)

References 42 publications

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“…LENS includes all the main features for TEM modeling: 1) flow perfusion; 2) a high cell permeable interface; 3) a 3D microenvironment; and 4) the possibility of detecting real‐time immune cell extravasation process in space and time. [ 172 ]…”

Section: Advanced Systems For In Vitro Modelingmentioning

confidence: 99%

Microbiota‐Host Immunity Communication in Neurodegenerative Disorders: Bioengineering Challenges for In Vitro Modeling

Boeri

Perottoni

Izzo

et al. 2021

Adv Healthcare Materials

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Human microbiota communicates with its host by secreting signaling metabolites, enzymes, or structural components. Its homeostasis strongly influences the modulation of human tissue barriers and immune system. Dysbiosis‐induced peripheral immunity response can propagate bacterial and pro‐inflammatory signals to the whole body, including the brain. This immune‐mediated communication may contribute to several neurodegenerative disorders, as Alzheimer's disease. In fact, neurodegeneration is associated with dysbiosis and neuroinflammation. The interplay between the microbial communities and the brain is complex and bidirectional, and a great deal of interest is emerging to define the exact mechanisms. This review focuses on microbiota‐immunity‐central nervous system (CNS) communication and shows how gut and oral microbiota populations trigger immune cells, propagating inflammation from the periphery to the cerebral parenchyma, thus contributing to the onset and progression of neurodegeneration. Moreover, an overview of the technological challenges with in vitro modeling of the microbiota‐immunity‐CNS axis, offering interesting technological hints about the most advanced solutions and current technologies is provided.

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Section: Advanced Systems For In Vitro Modelingmentioning

confidence: 99%

Microbiota‐Host Immunity Communication in Neurodegenerative Disorders: Bioengineering Challenges for In Vitro Modeling

Boeri

Perottoni

Izzo

et al. 2021

Adv Healthcare Materials

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“…Similar to cancer cells, leukocytes showed differences in the extravasation potential between different leukocyte subsets. Exploiting a microfluidic system allowing the physical separation of the extravascular and intravascular environment, a separate analysis of extravasated and non-extravasated neutrophils allowed studying the transcriptional profile of migratory and non-migratory neutrophil subsets (McMinn et al, 2019 ). Another model was applied to investigate the extravasation ability of different monocyte subsets, demonstrating that inflammatory monocytes have a superior TEM rate than patrolling monocytes (Boussommier-Calleja et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Advanced Microfluidic Models of Cancer and Immune Cell Extravasation: A Systematic Review of the Literature

Mondadori

Crippa

Moretti

et al. 2020

Front. Bioeng. Biotechnol.

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Extravasation is a multi-step process implicated in many physiological and pathological events. This process is essential to get leukocytes to the site of injury or infection but is also one of the main steps in the metastatic cascade in which cancer cells leave the primary tumor and migrate to target sites through the vascular route. In this perspective, extravasation is a double-edged sword. This systematic review analyzes microfluidic 3D models that have been designed to investigate the extravasation of cancer and immune cells. The purpose of this systematic review is to provide an exhaustive summary of the advanced microfluidic 3D models that have been designed to study the extravasation of cancer and immune cells, offering a perspective on the current state-of-the-art. To this end, we set the literature search cross-examining PUBMED and EMBASE databases up to January 2020 and further included non-indexed references reported in relevant reviews. The inclusion criteria were defined in agreement between all the investigators, aimed at identifying studies which investigate the extravasation process of cancer cells and/or leukocytes in microfluidic platforms. Twenty seven studies among 174 examined each step of the extravasation process exploiting 3D microfluidic devices and hence were included in our review. The analysis of the results obtained with the use of microfluidic models allowed highlighting shared features and differences in the extravasation of immune and cancer cells, in view of the setup of a common framework, that could be beneficial for the development of therapeutic approaches fostering or hindering the extravasation process.

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“…Particularly, an agarose-gelatin interpenetrating polymer network hydrogel was utilized to fabricate a tubular endothelialized fluidic system, in which sickle RBCs were loaded in the channels to investigate the endothelial barrier dysfunction in sickle cell disease [ 139 ]. Moreover, neutrophil, one of the types of white blood cells, was introduced in the endothelial tubes constructed in microfluidic channels with varying bifurcation angles [ 140 ] and mold-casted microchannels [ 141 , 142 ] to examine the interaction between endothelium and neutrophils. Beyond injecting each type of blood cells, whole human blood was infused into the tubular structure of endothelial barriers.…”

Section: Microvasculature-on-a-chipmentioning

confidence: 99%

Microvascularized tumor organoids-on-chips: advancing preclinical drug screening with pathophysiological relevance

et al. 2021

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Recent developments of organoids engineering and organ-on-a-chip microfluidic technologies have enabled the recapitulation of the major functions and architectures of microscale human tissue, including tumor pathophysiology. Nevertheless, there remain challenges in recapitulating the complexity and heterogeneity of tumor microenvironment. The integration of these engineering technologies suggests a potential strategy to overcome the limitations in reconstituting the perfusable microvascular system of large-scale tumors conserving their key functional features. Here, we review the recent progress of in vitro tumor-on-a-chip microfluidic technologies, focusing on the reconstruction of microvascularized organoid models to suggest a better platform for personalized cancer medicine.

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Neutrophil trafficking on-a-chip: an in vitro, organotypic model for investigating neutrophil priming, extravasation, and migration with spatiotemporal control

Abstract: Her we report a new microfluidic technology designed to facilitate the study of neutrophil trafficking and priming using primary human cells with a high degree of spatiotemporal control.

Cited by 30 publications

References 42 publications

Microbiota‐Host Immunity Communication in Neurodegenerative Disorders: Bioengineering Challenges for In Vitro Modeling

Microbiota‐Host Immunity Communication in Neurodegenerative Disorders: Bioengineering Challenges for In Vitro Modeling

Advanced Microfluidic Models of Cancer and Immune Cell Extravasation: A Systematic Review of the Literature

Microvascularized tumor organoids-on-chips: advancing preclinical drug screening with pathophysiological relevance

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