Neutrophil migration, oxidative metabolism and adhesion in early onset periodontitis

Biasi, Domenico; Bambara, Lisa Maria; Carletto, Antonio; Caramaschi, Paola; Andrioli, Giuseppe; Urbani, Giacomo; Bellavite, Paolo

doi:10.1034/j.1600-051x.1999.260901.x

Cited by 29 publications

(21 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of cellular defects have been reported in polymorphonuclear neutrophils (PMNs) from patients with aggressive periodontitis (AP) including decreased chemotaxis, 1,2 altered superoxide production, 3,4 decreased Ca 2+ mobilization 5 and decreased phagocytosis. 6 These altered properties of neutrophils are thought to account in part for the increased susceptibility of AP patients to infections by periodontal organisms.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients

et al. 2003

View full text Add to dashboard Cite

Polymorphonuclear neutrophils (PMNs) are attracted to sites of infection by N-formylpeptide (fMLP) chemoattractants. The high-affinity fMLP receptor (FPR1) of phagocytic cells interacts with bacterial fMLP and mediates chemotaxis, degranulation, and superoxide production. These cellular functions are disrupted in PMN from aggressive periodontitis (AP) patients. Two FPR1 gene single nucleotide polymorphisms (SNPs), c.329T4C and c.378C4G, have been associated with a localized form of AP in African-American patients. To evaluate the generality of these SNPs in AP patients, we sequenced a 363 bp interval of the FPR1 gene in an ethnically diverse group of patients (n ¼ 111) and controls (n ¼ 115). Neither c.329T4C nor c.378C4G were detected in the 452 alleles sequenced. Six SNPs were identified including two located in the FPR1 second extracellular loop that were significantly associated with the AP phenotype in African-American patients (p.R190W, P ¼ 0.0033; and p.N192K, P ¼ 0.0018). These two SNPs show three predominant haplotypes, each associated with a different disease risk in African-Americans. These data do not support the hypothesis that the FPR1 SNPs c.329T4C and c.378C4G play an etiologic role in aggressive periodontitis, but do suggest that SNPs in the second extracellular loop may be etiologically important.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients

et al. 2003

View full text Add to dashboard Cite

show abstract

“…They are further capable of generating chemostatic factors from plasma via activation of the complement, coagulation, fibrinolytic and kinin systems, or from surrounding cells following interactions with cellular receptors [44]. The accumulation of these cells at the sites of inflammation is accompanied by modification of their activity/ability to release granule content and reactive oxygen species [5]. Matthews et al [6] hypothesized that peripheral neutrophils in periodontitis also show both hyperreactivity to plaque organisms and hyperactivity in terms of the baseline, unstimulated generation and release of ROS.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors report significantly increased [6] luminol-dependent CL of the venous blood activated PMN taken from periodontitis patients, whereas other authors [5] – especially those who investigated the oxidative function of the venous blood activated PMN in diabetes mellitus via luminol-dependent CL [46] – found it to be significantly decreased (p<0.01). The differences in the study results may be due the usage of different substances for the activation of the PMN, different methods applied for isolation of these cells, or other factors.…”

Section: Discussionmentioning

confidence: 99%

“…The accumulation of these cells at the sites of inflammation is accompanied by modification of their activity/ability to release granule content and reactive oxygen species (ROS) [5]. Matthews et al [6] hypothesized that peripheral neutrophils in periodontitis also show both hyperreactivity to plaque organisms and hyperactivity in terms of the baseline, unstimulated generation and release of ROS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S

Žilinskas

Žekonis

et al. 2011

Med Sci Monit

View full text Add to dashboard Cite

SummaryBackgroundThe anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.Material/MethodsThe study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).ResultsPolymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10−4 of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.ConclusionsThis study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

show abstract

“…62 Of the various reported PMN anomalies, one finding related to altered fMLPmediated chemotaxis has been described in some patients with the juvenile form of disease. It is associated with severe and protracted gingival infections leading to tooth loss.…”

Section: β-Actin Gene Defectmentioning

confidence: 99%

Neutrophil Migration Defects

Hanna

2014

Stiehm's Immune Deficiencies

View full text Add to dashboard Cite

Neutrophil migration, oxidative metabolism and adhesion in early onset periodontitis

Cited by 29 publications

References 24 publications

Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients

Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients

Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S

Neutrophil Migration Defects

Contact Info

Product

Resources

About