Neutrophil migration across model intestinal epithelia: Monolayer disruption and subsequent events in epithelial repair

Nusrat, Asma; Parkos, Charles A.; Liang, Tony W.; Carnes, Denice K.; Madara, James L.

doi:10.1053/gast.1997.v113.pm9352851

Cited by 119 publications

(98 citation statements)

References 6 publications

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“…Accordingly, the chemotactic gradient results in a remarkable migration of neutrophils. 37,38 The elevated colon MPO activity, which is reported in the present study is consisting with pathogenesis. Moreover, it is worthwhile to conclude that the HBE exerts its anti-inflammatory and gastroprotective effects at least partly by decreasing the MPO activity and preventing further epithelial injuries.…”

Section: Discussionsupporting

confidence: 73%

Comparative Protective Effect of Hawthorn Berry Hydroalcoholic Extract, Atorvastatin, and Mesalamine on Experimentally Induced Colitis in Rats

Malekinejad

Shafie-Irannejad

Hobbenaghi

et al. 2013

Journal of Medicinal Food

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The protective effect of hydroalcoholic extract of hawthorn berries (HBE) on acetic acid (AA)-induced colitis in rats was investigated. Forty-two Wistar rats were divided into seven groups, including control and test groups (n = 6). The control animals received saline, and the test animals were treated with saline (sham group), mesalamine (50 mg/kg; M group), atorvastatin (20 mg/kg; A group), HBE (100 mg/kg; H group), mesalamine and HBE (HM group), or atorvastatin plus HBE (HA group), 3 days before and a week after colitis induction. Colitis was induced by administration of 1 mL AA (4%) via a polyethylene catheter intrarectally. High-performance liquid chromatography analyses showed that HBE contained 0.13% and 0.5% oleanolic acid and ursolic acid, respectively. Elevated myeloperoxidase activity and lipid peroxidation were attenuated in the HA group. The H and HM groups showed marked reductions in colitis-induced decreases in total thiol molecules and body weight. The histopathological studies revealed that HBE decreased colitis-induced edema and infiltration of neutrophils. Our data suggest the anti-inflammatory and antioxidant effects of HBE and atorvastatin protect against AA-induced colitis. The anti-inflammatory effect of HBE may be attributable to its ability to decrease myeloperoxidase activity as a biomarker of neutrophil infiltration.

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Section: Discussionsupporting

confidence: 73%

Comparative Protective Effect of Hawthorn Berry Hydroalcoholic Extract, Atorvastatin, and Mesalamine on Experimentally Induced Colitis in Rats

Malekinejad

Shafie-Irannejad

Hobbenaghi

et al. 2013

Journal of Medicinal Food

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“…It is well accepted that in many inflammatory disorders of the gastrointestinal tract, the combination of epithelial injury, disease activity and symptoms parallel PMN infiltration of the mucosa (Hawker et al 1980, Weiland et al 1986, Koyama et al 1991, Nusrat et al 1997. The etiology of disease symptoms associated with PMN transepithelial migration is complex but a major component is related to the effects of PMNs on the epithelial barrier and on epithelial cell function.…”

Section: Leukocyte-epithelial Interactions and Disease Pathophysiologymentioning

confidence: 99%

“…The etiology of disease symptoms associated with PMN transepithelial migration is complex but a major component is related to the effects of PMNs on the epithelial barrier and on epithelial cell function. For example, while studies have shown that passage of a single PMN across epithelial tight junctions results in rapid resealing with little loss of barrier function (Milks et al 1983, Nash et al 1987, Parsons et al 1987), large scale PMN migration results in the formation of sizable epithelial discontinuities (Nusrat et al 1997) that likely represent the precursors of epithelial erosions and ulcers characteristic of many of the inflammatory conditions mentioned above. Direct effects of PMNs on epithelial cell function appear to be mediated both by cell-cell contact and by release of factors that initiate subsequent signaling events.…”

Section: Leukocyte-epithelial Interactions and Disease Pathophysiologymentioning

confidence: 99%

Neutrophil transepithelial migration: role of toll-like receptors in mucosal inflammation

Reaves

Chin

Parkos

2005

Mem. Inst. Oswaldo Cruz

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“…Although neutrophilic inflammation was not responsible for severity of villus atrophy or loss of epithelial continuity in C. parvum-infected mucosa, neutrophils may biochemically and physically compromise intestinal barrier function by disrupting epithelial tight junctions (29). We have shown previously that C. parvum infection results in a loss of barrier function that is compensated for by an increase in mucosal synthesis of prostaglandins (12).…”

Section: Discussionmentioning

confidence: 89%

Neutrophils Do Not Mediate the Pathophysiological Sequelae of Cryptosporidium parvum Infection in Neonatal Piglets

Zadrozny¹,

Stauffer²,

Armstrong³

et al. 2006

Infect Immun

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Cryptosporidium parvum is a minimally invasive protozoal pathogen of intestinal epithelium that results in villus atrophy, mucosal lipid peroxidation, diarrhea, and diminished barrier function. Influx of neutrophils is a consistent feature of human and animal cryptosporidiosis, and yet their contribution to the pathological sequelae of infection has not been investigated. Accordingly, we used an established neonatal piglet model of C. parvum infection to examine the role of neutrophils in disease pathogenesis by inhibiting their recruitment and activation in vivo using a monoclonal anti-CD18 antibody. Infected piglets were treated daily with anti-CD18 or isotype control immunoglobulin G and euthanized at peak infection, at which time neutrophil infiltrates, lipid peroxidation, severity of infection, and intestinal barrier function were quantified. C. parvum infection resulted in a significant increase in mucosal neutrophil myeloperoxidase activity that was prevented by treatment of piglets with anti-CD18 antibody. Neutrophil recruitment was dependent on mucosal superoxide formation (prevented by treatment of infected piglets with superoxide dismutase). Neutrophils did not contribute to peroxynitrite formation or peroxidative injury of C. parvum-infected mucosa and had no impact on the severity of epithelial infection, villus atrophy, or diarrhea. The presence of neutrophils in C. parvuminfected mucosa was associated with enhanced barrier function that could not be attributed to mucosal elaboration of prostaglandins or stimulation of their synthesis. These studies are the first to demonstrate that neutrophilic inflammation arising in response to infection by a noninvasive epithelial pathogen results in physiologic rather than pathological effects in vivo.

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Neutrophil migration across model intestinal epithelia: Monolayer disruption and subsequent events in epithelial repair

Cited by 119 publications

References 6 publications

Comparative Protective Effect of Hawthorn Berry Hydroalcoholic Extract, Atorvastatin, and Mesalamine on Experimentally Induced Colitis in Rats

Comparative Protective Effect of Hawthorn Berry Hydroalcoholic Extract, Atorvastatin, and Mesalamine on Experimentally Induced Colitis in Rats

Neutrophil transepithelial migration: role of toll-like receptors in mucosal inflammation

Neutrophils Do Not Mediate the Pathophysiological Sequelae of Cryptosporidium parvum Infection in Neonatal Piglets

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