Neutrophil Granulocyte Function in Bacterial Infections

Solberg, Claus Ola; Hellum, Kjell B.

doi:10.1016/s0140-6736(72)92022-3

Cited by 112 publications

(47 citation statements)

References 11 publications

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“…Two groups of investigators previously have observed neutrophil bactericidal defects from patients with bacterial infections using techniques similar to those described here (6,7). Both of their reports showed only slight differences between patients and controls (approximately 5%), a defect not considered significant in our laboratory.…”

Section: Resultssupporting

confidence: 60%

“…More recently, increased susceptibility to infection has been reported in patients with morphologically normal neutrophils and normal or elevated leukocyte counts. Impaired functional capacity of PMNLs has been observed in studies of patients with genetically inherited syndromes (1-4), neoplastic diseases (23,24), metabolic diseases (25,26), immunologic disorders (27,28), surgical diseases (29), extensive burns (30), and severe infection (5)(6)(7)(8)(9). The extent to which this occurs and th-e nature of the underlying defects remain controversial.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, several investigators have described other syndromes of neutrophil dysfunction in which a defect is presumably "acquired" before or during infection and is occasionally reversible. Leukocytes from some acutely infected patients have demonstrated diminished responses in studies of their leukotactic, phagocytic, metabolic, and microbicidal activity, but other studies have shown these functions to be normal or increased (5)(6)(7)(8)(9)(10). The rapidly changing clinical circumstances in severely ill patients and differences in the methodology used in such studies have made it difficult to evaluate these divergent conclusions.…”

mentioning

confidence: 99%

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Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

Weinstein¹,

Young²

1976

J. Clin. Invest.

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A B S T R A C T To assess the phagocytic and bactericidal function of neutrophils in the acute stages of gramnegative rod bacteremia, cells from 30 nonleukopenic patients were studied in a test system utilizing plasma obtained simultaneously with culture-positive blood, the autologous infecting strain, and two laboratory test strains of Staphylococcus aureus and Pseudomonas aeruginosa. Results were compared to those obtained with normal neutrophils and plasma. Patient and control plasma were simultaneously tested with each source of phagocytic cells to localize any abnormalities. Four patients had a defect against their infecting strain, 33% of the inoculum phagocytized and killed versus 80% by controls. In these cases differences were localized to the patients' plasma, as normal plasma tested with patients' cells reversed the defect. Thus, four patients had impaired opsonization when compared to normal controls, but we also observed that 11 of 30 bacteremic isolates, all Escherichia coli, showed absolute or relative resistance to phagocytosis in the patient and control assay system. No intrinsic granulocyte killing abnormalities were noted. There was poor correlation between results obtained with infecting strains compared to laboratory test organisms. We conclude that in patients without evidence of an inherited neutrophil bactericidal disorder, recurrent infection, or treatment with cytotoxic drugs, intrinsic bactericidal defects are uncommon at the onset of gram-negative bacteremia, and impaired opsonization is the most commonly encountered cause of neutrophil dysfunction.

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

Weinstein¹,

Young²

1976

J. Clin. Invest.

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“…What happens to PMN in patients with acute bacterial infections is less clear. Both increased (3,4), normal and decreased (5,6) phagocytic capabilities of PMN in patients with infection have been reported. In general, these studies used serum-opsonized particles as phagocytic targets.…”

Section: Introductionmentioning

confidence: 96%

Studies on phagocytosis in patients with acute bacterial infections.

Simms¹,

Frank

Quinn

et al. 1989

J. Clin. Invest.

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Polymorphonuclear leukocytes (PMN) and monocytes from 20 patients with acute bacterial infections were examined for phagocytic function. PMN of patients expressed markedly enhanced phagocytosis as measured by the ingestion of erythrocyte (E)IgG and IgG/C3b-coated E. Phagocytosis of E coated with C3b alone was not seen, while low levels of ingestion of iC3b-E by patients' PMNs was noted. Monocytes from patients and controls expressed similar phagocytic activity in a fixed endpoint assay; however, the kinetics of phagocytosis by patients' monocytes was strikingly faster. Superoxide anion (02) and myeloperoxidase activities were similar to controls in PMN of four patients studied on day 1 of admission.PMN from two of three patients studied longitudinally showed an initial elevation in EIgG phagocytosis, which fell to normal levels by day 4, concomitantly with increased 0°gener-ation and clinical improvement. Phagocytosis remained elevated in the third patient who did not clear his septicemia.

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“…The recruitment to and activity of PMNs in the lung are a crucial component of the host defense against pneumonia, since PMNs eliminate bacteria via phagocytosis [2]. Acquired impairments of PMN functions were described in patients with severe infection and in patients with comorbidities many years ago causing reduced bacterial killing and impaired migration to infected sites [3, 4]. In addition a relatively low neutrophil count (<9/nl, not neutropenic) in pneumonia has been associated with higher mortality [5].…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Apoptosis, Activation and Anti-Inflammatory Cytokine Response in Granulocyte Colony-Stimulating Factor-Treated Patients with Community-Acquired Pneumonia

Droemann

Hansen²,

Aries³

et al. 2006

Respiration

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Background:Despite antibiotic treatment, the mortality of severe community-acquired pneumonia (CAP), especially in patients with severe comorbidity, remains high. Innate defense mechanisms including polymorphonuclear neutrophil (PMN) activation and survival, orchestrated by cytokines, are primarily responsible for the elimination of bacterial organisms from the alveolus. Objectives: The aim of this study was to evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on PMN activation, apoptosis and cytokine response in patients with CAP. Methods: Patients received a single dose of G-CSF (1 × 300 or 480 µg s.c.) prior to standard antibiotic treatment (n = 8) or standard treatment only (n = 8). Apoptosis rate and expression of CD11b, CD66b, CD64 and CD114 surface molecules on systemic PMN were assessedusing fluorescence-activated cell sorter analysis. Levels of the interleukin-1 receptor antagonist (IL-1RA), the soluble tumor necrosis factor receptor inhibitor (sTNF-p55) and G-CSF were measured by ELISA. Results: In the treatment group, 12 h after G-CSF application, neutrophil count increased, neutrophil activation marker CD11b was stimulated (CD11b: 48.6 ± 9.7 vs. 71.2 ± 17.7, p < 0.01), neutrophil apoptosis decreased (apoptosis: 1.36 ± 0.27 vs. 0.2 ± 0.12%, p < 0.01) and the concentration of IL-1RA and sTNF-p55 increased (IL-1RA 136.4 ± 72.2 vs. 340.1 ± 194.6 ng/ml, p < 0.01; sTNF-p55 382 ± 4,243 vs. 632 ± 4,714 ng/ml, p < 0.01; control group nonsignificant). These effects were not seen in the control group. Conclusions: The application of a single dose of G-CSF in patients with CAP caused a prolonged survival and increased activation of neutrophils combined with a sustained release of anti-inflammatory cytokines.

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Neutrophil Granulocyte Function in Bacterial Infections

Cited by 112 publications

References 11 publications

Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

Studies on phagocytosis in patients with acute bacterial infections.

Neutrophil Apoptosis, Activation and Anti-Inflammatory Cytokine Response in Granulocyte Colony-Stimulating Factor-Treated Patients with Community-Acquired Pneumonia

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