Neutrophil extracellular trap release driven by bacterial motility: Relevance to cystic fibrosis lung disease

Rada, Balázs

doi:10.1080/19420889.2017.1296610

Cited by 17 publications

(18 citation statements)

References 30 publications

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“…Not only are neutrophils present in massive amounts, but infectious stimuli for NETosis are also abundant. Exposure of neutrophils to either LPS or P aeruginosa stimulated NETosis; however, over time, P aeruginosa became resistant to NET‐mediated killing . Interestingly, motile, flagellated P aeruginosa were more likely to induce NETosis than were immotile, non‐flagellated P aeruginosa , the most common form found in CF lung biofilms .…”

Section: Cellular Contributions To the Cf Airway Inflammatory Responsementioning

confidence: 99%

Inflammation in cystic fibrosis: An update

Roesch

Nichols

Chmiel

2018

Pediatric Pulmonology

197

221

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Inflammation plays a critical role in cystic fibrosis (CF) lung pathology and disease progression making it an active area of research and important therapeutic target. In this review, we explore the most recent research on the major contributors to the exuberant inflammatory response seen in CF as well as potential therapeutics to combat this response. Absence of functional cystic fibrosis transmembrane conductance regulator (CFTR) alters anion transport across CF airway epithelial cells and ultimately results in dehydration of the airway surface liquid. The dehydrated airway surface liquid in combination with abnormal mucin secretion contributes to airway obstruction and subsequent infection that may serve as a trigger point for inflammation. There is also evidence to suggest that airway inflammation may be excessive and sustained relative to the infectious stimuli. Studies have shown dysregulation of both pro-inflammatory mediators such as IL-17 and pro-resolution mediators including metabolites of the eicosanoid pathway. Recently, CFTR potentiators and correctors have garnered much attention in the CF community. Although these modulators address the underlying defect in CF, their impact on downstream consequences such as inflammation are not known. Here, we review pre-clinical and clinical data on the impact of CFTR modulators on inflammation. In addition, we examine other cell types including neutrophils, macrophages, and T-lymphocytes that express CFTR and contribute to the CF inflammatory response. Finally, we address challenges in developing anti-inflammatory therapies and highlight some of the most promising anti-inflammatory drugs under development for CF.

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Section: Cellular Contributions To the Cf Airway Inflammatory Responsementioning

confidence: 99%

Inflammation in cystic fibrosis: An update

Roesch

Nichols

Chmiel

2018

Pediatric Pulmonology

197

221

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“…De la misma forma, es posible que esta proteína esté involucrada en la mediación inicial de la interacción con la superficie de la vía aérea uniéndose con el glicoesfingolípido asialo M1 (aGM1) de las células epiteliales 17 . Pseudomonas aeruginosa también cuenta con la proteína flagelar FliC, principal factor que desencadena la secreción de péptidos antimicrobianos por el sistema inmune, liberación de trampas extracelulares mediadas por neutrófilos (NET´s) 18…”

Section: Factores De Patogenicidad Asociados a La Célula Bacterianaunclassified

“…Genes o proteínas involucradas Patogenia y además, posee la capacidad de ser reconocida a través de los receptores tipo Toll (TLR) tipo 5 (TLR5) 19 teniendo como consecuencia una expresión de IL-18 e IL-1B, lo que puede desencadenar a una muerte celular piroptótica mediante la vía de vía NFkB (factor nuclear kappaacelerador de la cadena ligera de células B activadas) 20,21 . Para evitar la inducción de la respuesta inflamatoria y la acción de NET's, P. aeruginosa suprime la regulación del gen que codifica para la flagelina cambiando su fenotipo a una cepa mucoide; para ello induce la producción del polisacárido alginato, el que se asocia a daño parenquimatoso 18,22 .…”

Section: Factoresunclassified

Pseudomonas aeruginosa: patogenicidad y resistencia antimicrobiana en la infección urinaria

Paz-Zarza¹,

Mangwani-Mordani²,

Martínez-Maldonado³

et al. 2019

Rev. chil. infectol.

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Among the most frequent nosocomial infections associated with polyresistant bacteria and with a worse prognosis, are those produced by Pseudomonas aeruginosa. This bacterium has a high capacity to adapt to adverse conditions such as pH and osmolarity of urine. Pseudomonas aeruginosa is one of the main pathogens involved in nosocomial infections and immunosuppressed patients. This bacterium is considered an opportunistic infectious agent that has diverse mechanisms of pathogenicity, as well as resistance to antimicrobials, which contributes to the difficulty in the treatment of these infections. In the present bibliographic review, the taxonomy, pathogenicity mechanisms and resistance genes of P. aeruginosa are analyzed. Likewise, the micro-environmental factors of the urinary infection produced by this bacterium are approached, making an approach to the understanding of the pathophysiological bases of this infection.

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“…Airway epithelial cells express DUOX1 and DUOX2 proteins in their apical plasma membrane that are the major H 2 O 2 sources for the H 2 O 2 /LPO/SCN antimicrobial system (Geiszt et al, 2003;Rada, 2017). Air-liquid cultures of differentiated tracheobronbchial epithelial cells provide an excellent, cell-based, in vitro model to study the DUOX/ H 2 O 2 /LPO/SCN − antimicrobial system (Conner et al, 2002;.…”

Section: Antibacterial Effects Of the Duox/ H 2 O 2 /Lpo Systemmentioning

confidence: 99%

Antimicrobial actions of dual oxidases and lactoperoxidase

et al. 2018

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The NOX/DUOX family of NADPH oxidases are transmembrane proteins generating reactive oxygen species as their primary enzymatic products. NADPH oxidase (NOX) 1-5 and Dual oxidase (DUOX) 1 and 2 are members of this family. These enzymes have several biological functions including immune defense, hormone biosynthesis, fertilization, cell proliferation and differentiation, extracellular matrix formation and vascular regulation. They are found in a variety of tissues such as the airways, salivary glands, colon, thyroid gland and lymphoid organs. The discovery of NADPH oxidases has drastically transformed our view of the biology of reactive oxygen species and oxidative stress. Roles of several isoforms including DUOX1 and DUOX2 in host innate immune defense have been implicated and are still being uncovered. DUOX enzymes highly expressed in the respiratory and salivary gland epithelium have been proposed as the major sources of hydrogen peroxide supporting mucosal oxidative antimicrobial defenses. In this review, we shortly present data on DUOX discovery, structure and function, and provide a detailed, up-to-date summary of discoveries regarding antibacterial, antiviral, antifungal, and antiparasitic functions of DUOX enzymes. We also present all the literature describing the immune functions of lactoperoxidase, an enzyme working in partnership with DUOX to produce antimicrobial substances.

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Neutrophil extracellular trap release driven by bacterial motility: Relevance to cystic fibrosis lung disease

Cited by 17 publications

References 30 publications

Inflammation in cystic fibrosis: An update

Inflammation in cystic fibrosis: An update

Pseudomonas aeruginosa: patogenicidad y resistencia antimicrobiana en la infección urinaria

Antimicrobial actions of dual oxidases and lactoperoxidase

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