Neutrophil Extracellular Trap–Associated CEACAM1 as a Putative Therapeutic Target to Prevent Metastatic Progression of Colon Carcinoma

Rayes, Roni F.; Vourtzoumis, Phil; M, Bou Rjeily; Seth, Rashmi; Bourdeau, France; Giannias, Betty; Bérubé, Julie; Huang, Yi Hsiang; Rousseau, Stéphane; Camilleri-Broët, Sophie; Blumberg, RS; Beauchemin, Nicole; Najmeh, Sara; Cools-Lartigue, Jonathan; Jd, Spicer; Ferri, LE

doi:10.4049/jimmunol.1900240

Cited by 61 publications

(63 citation statements)

References 51 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these 84 genes, 16 genes were NETs associated genes ( Figure 5B; Table 3) (33-46) Of the 16 genes, LGALS9, HCK, LCP1, CEACAM1 were involved in the cytokine-mediated signaling pathway. S100A8, LGALS9, and CTSC were involved in regulation of apoptotic signal by enrichment annotation from the Metascape tool ( Figure 5B; Table 3) (33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46).…”

Section: Neutrophil Activation Related Genes Enrichment Analysismentioning

confidence: 99%

Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19

et al. 2020

View full text Add to dashboard Cite

Background: Cases of excessive neutrophil counts in the blood in severe coronavirus disease (COVID-19) patients have drawn significant attention. Neutrophil infiltration was also noted on the pathological findings from autopsies. It is urgent to clarify the pathogenesis of neutrophils leading to severe pneumonia in COVID-19. Methods: A retrospective analysis was performed on 55 COVID-19 patients classified as mild (n = 22), moderate (n = 25), and severe (n = 8) according to the Guidelines released by the National Health Commission of China. Trends relating leukocyte counts and lungs examined by chest CT scan were quantified by Bayesian inference. Transcriptional signatures of host immune cells of four COVID19 patients were analyzed by RNA sequencing of lung specimens and BALF. Results: Neutrophilia occurred in 6 of 8 severe patients at 7-19 days after symptom onset, coinciding with lesion progression. Increasing neutrophil counts paralleled lesion CT values (slope: 0.8 and 0.3-1.2), reflecting neutrophilia-induced lung injury in severe patients. Transcriptome analysis revealed that neutrophil activation was correlated with 17 neutrophil extracellular trap (NET)-associated genes in COVID-19 patients, which was related to innate immunity and interacted with T/NK/B cells, as supported by a protein-protein interaction network analysis. Conclusion: Excessive neutrophils and associated NETs could explain the pathogenesis of lung injury in COVID-19 pneumonia.

show abstract

Section: Neutrophil Activation Related Genes Enrichment Analysismentioning

confidence: 99%

Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Related factors released by neutrophils, such as interleukin (IL)-17, can promote the EMT process of tumor cells via the JAK2/STAT3 pathway ( 24 ). NETs have been reported to be able to promote tumor cell adhesion and thus promote tumor cell progression and metastasis ( 25 ). In the present study, a large amount of NETs formation was found in the plasma of patients with advanced GC and in GC tumor tissues; however, the interaction between tumor and NETs needs to be further investigated.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil extracellular traps promote gastric cancer metastasis by inducing epithelial‑mesenchymal transition

Zhu

Zou

Yang³

et al. 2021

Int J Mol Med

View full text Add to dashboard Cite

The risks of tumor recurrence following the successful resection of the primary tumor have been known for decades; however, the precise mechanisms underlying treatment failures remain unknown. The formation of neutrophil extracellular traps (NETs) has increasingly been demonstrated to be associated with thrombi formation in cancer patients, as well as with the development and metastasis of cancer. The present study demonstrated that the level of peripheral blood NETs in patients with gastric cancer (Gc) was associated with tumor progression, and patients with stage III/IV disease exhibited significant differences compared with the healthy controls and patients with stage I/II disease, which may be associated with an increased risk of metastasis. In addition, plasma from patients with stage III/IV Gc was more prone to stimulate neutrophils to form NETs; thus, it was hypothesized that the formation of NETs may be affected by the tumor microenvironment. A higher deposition of NETs in Gc tissues compared with normal resection margins was also identified. In vitro, following treatment with phorbol myristate acetate, which promotes the formation of NETs, or with dNAse-1/GSK-484, which inhibits the formation of NETs, it was found that the tumor migratory ability was altered; however, no significant changes were observed in cell proliferation and cell cycle progression. Epithelial-mesenchymal transition (EMT) is a key event associated with dissemination and metastasis in Gc pathogenesis. Finally, the present study demonstrated that NETs promote a more aggressive mesenchymal phenotype and promote the progression of Gc in vitro and in vivo. On the whole, to the best of our knowledge, the present study reports a previously unknown role of NETs in the regulation of Gc, which is associated with EMT and migration. Therefore, targeting NETs may prove to be therapeutically beneficial.

show abstract

“…P ≤ 0.05 was considered statistically significant, and P ≤ 0.01 as highly significant. Pearson correlation coefficient lower than 0.4 indicates poor reproducibility, which range from 0.4–0.75, denoting fair reproducibility, and equal to 0.75 indicates good reproducibility 17 . ( M represents the median of the data).…”

Section: Methodsmentioning

confidence: 99%

Eosinophils may serve as CEA-secreting cells for allergic bronchopulmonary aspergillosis (ABPA) patients

Yang

Gao

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterized by an exaggerated response of the immune system to the fungus Aspergillus. This study aimed to assess the relationship between carcinoembryonic antigen (CEA) and eosinophils in ABPA patients. We describes a case of a 50-year-old patient who was diagnosed with ABPA presenting with high level of CEA and eosinophils. Besides,we used immunohistochemistry and immunofluorescence to identify eosinophils and CEA in sections which were obtained by Endobronchial ultrasound-guided transbronchial lung biopsy aspiration (EBUS-TBLB). The sections were then visualized using confocal microscopy. We also retrospectively analyzed a cohort of 37 ABPA patients between January 2013 and December 2019 in our hospital. We found the patient whose serum CEA levels were consistent with eosinophils during the follow-up (r = 0.929, P = 0.022). The positive expression of CEA and abnormal expression of eosinophils was higher in the ABPA tissue compared to the normal lung tissue. The co-localization was represented as pixels containing both red and green color in the image (with various shades of orange and yellow) which signified that eosinophils were immunohistochemically positive for CEA. Patients with higher levels of eosinophils had higher levels of CEA in the serum (P < 0.001). The results of Pearson correlation analysis showed that the levels of eosinophils were positively correlated with serum CEA levels (r = 0.459 and r = 0.506, P = 0.004 and P = 0.001). Serum CEA level is elevated in ABPA patients. The elevated serum CEA level was shown to be normalized after treatment. Increased CEA levels in ABPA patients may be positively correlated with eosinophil levels, and eosinophils may be served as CEA-secreting cells in patients with ABPA.

show abstract

Neutrophil Extracellular Trap–Associated CEACAM1 as a Putative Therapeutic Target to Prevent Metastatic Progression of Colon Carcinoma

Cited by 61 publications

References 51 publications

Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19

Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19

Neutrophil extracellular traps promote gastric cancer metastasis by inducing epithelial‑mesenchymal transition

Eosinophils may serve as CEA-secreting cells for allergic bronchopulmonary aspergillosis (ABPA) patients

Contact Info

Product

Resources

About