Neutrophil elastase, von willebrand factor, soluble thrombomodulin and percutaneous oxygen in peripheral atherosclerosis

Blann, AD; Seigneur, M.; Adams, Rachel A.; McCollum, Charles

doi:10.1016/s1078-5884(96)80110-9

Cited by 35 publications

(21 citation statements)

References 19 publications

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“…The means of total leukocyte count and counts of all leukocyte subtypes in the extreme hypertriglyceridemic group were all higher than those in the pure hypertriglyceridemic group, although only the differences in total leukocyte count and neu- trophil count reached statistical significances (see data in the last paragraph of the Results section). There is accumulating evidence from human studies that hypertriglyceridemia is a risk factor for atherosclerosis and CVD (6,7), and it seems likely that different leukocytes play different roles in atherogenesis (8)(9)(10)20). A higher peripheral total leukocyte count is associated with a higher risk of CVD (3,(11)(12)(13) and the association of hypertriglyceridemia with a higher CVD risk …”

Section: Discussionmentioning

confidence: 99%

“…There is little information concerning this issue so far in the literature. An in-depth study on this point may help in understanding the pathophysiology of hyperlipidemia-induced atherosclerosis, since accumulating evidence from human studies has shown that hyperlipidemia is a major cause of atherosclerosis and cardiovascular disease (CVD) (5)(6)(7), that different types of leukocytes are likely to play important but different roles in atherosclerosis (8)(9)(10), and that a higher peripheral total leukocyte count is associated with an increased incidence of CVD (3,(11)(12)(13). In addition, because cigarette smoking and obesity are frequently found in association with both lipid disorders (14-16) and a higher peripheral leukocyte count (1,3,13,(17)(18)(19), we included the factors of smoking and body mass index (BMI) in the study.…”

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Peripheral differential leukocyte counts in humans vary with hyperlipidemia, smoking, and body mass index

Huang

Chien

Yang

et al. 2001

Lipids

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Reports of diverse relationships between blood concentrations of different lipids and peripheral total leukocyte count, and a unique lower peripheral monocyte count in hypercholesterolemia, have driven us to think that in humans, peripheral differential leukocyte counts may be influenced differently by different types of hyperlipidemia. Our subjects were Taipei residents who attended a regular health check program in our hospital in 1998. A total of 3,282 subjects was enrolled, including 1,677 normolipidemic, 960 untreated borderline hyperlipidemic, and 645 untreated hyperlipidemic subjects. By one-way analysis of variance (ANOVA), we found that different types of hyperlipidemia were associated with significant differences in differential leukocyte counts. In hypertriglyceridemia, the total leukocyte count and counts of all leukocyte subtypes were significantly higher than those in normolipidemia. Pure hypercholesterolemia, by contrast, was associated with a significantly lower monocyte count and no significant difference in other leukocyte counts. By two-way ANOVA adjusted for presence and degree of hyperlipidemia, we found significantly higher counts of total leukocytes and of all leukocyte subtypes in smokers, and significantly positive trends in relationships between body mass index (BMI) and counts of all leukocytes, neutrophils, lymphocytes, and monocytes. By multivariate regression analysis including all subjects, the serum triglyceride (TG) level was positively correlated with total leukocyte count and counts of all subtypes except eosinophils. On the contrary, serum high density lipoprotein-cholesterol had a negative correlation with total leukocyte count and with counts of neutrophils, monocytes, and basophils. In these multivariate regression analyses, there was no significant correlation between lipid levels and eosinophil count, whereas smoking was consistently associated with significantly higher counts of all leukocyte subtypes, including eosinophils. BMI had a significantly positive correlation with counts of all leukocytes, neutrophils, lymphocytes, and monocytes.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Peripheral differential leukocyte counts in humans vary with hyperlipidemia, smoking, and body mass index

Huang

Chien

Yang

et al. 2001

Lipids

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“…These may contribute to diseases such as atherosclerosis, myocardial infarction, stroke and peripheral vascular disease, and may cause host cell damage following surgery involving the application of a tourniquet. 16,17 Previous studies have used animal and human models of ischaemia-reperfusion injury. [18][19][20][21] Leucocytes are involved intrinsically in the inflammatory reaction and thus deserve research attention.…”

Section: Introductionmentioning

confidence: 99%

“…16,28 Statistical analysis Results were presented as mean ± standard deviation (SD). Changes in the measured parameters during ischaemia and reperfusion were determined by repeated measures analysis of variance (ANOVA).…”

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confidence: 99%

Role of leucocytes in damage to the vascular endothelium during ischaemia-reperfusion injury

Hughes¹,

Cotter

Evans

et al. 2006

British Journal of Biomedical Science

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During this investigation, a model of tourniquet-induced forearm ischaemia-reperfusion injury is employed to investigate the role of leucocytes in damage to the vascular endothelium during ischaemia-reperfusion injury. Leucocyte entrapment is investigated by measuring the concentration of leucocytes in venous blood leaving the arm. Neutrophil and monocyte leucocyte subpopulations are isolated by density gradient centrifugation techniques. Cell surface expression of CD11b and the intracellular production of hydrogen peroxide are measured via flow cytometry. Plasma concentrations of elastase and von Willebrand factor (vWF) are measured using enzyme-linked immunosorbemt assay (ELISA) techniques. During ischaemia-reperfusion, there was an increase in CD11b cell surface expression on neutrophils (P=0.040) and monocytes (P=0.049), and a decrease in peripheral blood leucocytes (P=0.019). There was an increase in the intracellular production of hydrogen peroxide by leucocyte subpopulations (P=0.027 [neutrophils], P=0.091 [monocytes]) and in the plasma elastase concentration (P=0.05). There was also a trend to increasing plasma concentration of vWF (P=0.0562), which was measured as a marker of endothelial damage. Ischaemia-reperfusion results in increased adhesiveness, entrapment and activation of leucocytes. Even following a mild ischaemic insult, this leucocyte response was followed immediately by evidence of endothelial damage. These results may have important implications for understanding the development of chronic diseases that involve mild ischaemic episodes.

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“…There is abundant evidence that activation of this system occurs in chronic pulmonary disease [7,8] in which inflammation plays a role. More recently evidence has emerged that indicates that elastase activity may be related to endothelial cell damage [9] . This opens the door for an involvement in vascular disease and to support this, in a rat heart model, inhibition of neutrophil elastase was associated with cardioprotection from repetitive ischaemia and myocardial infarction [10] .…”

Section: See Page 1607 For the Article To Which This Editorial Refersmentioning

confidence: 99%

The Edinburgh Artery Study: is lytic activity bad for the heart?

Grant

2000

European Heart Journal

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Neutrophil elastase, von willebrand factor, soluble thrombomodulin and percutaneous oxygen in peripheral atherosclerosis

Abstract: Our data support the hypothesis that release of elastase from activated neutrophils relates to endothelial cell damage. This may contribute to hypoxia and may result in the deterioration in clinically assessed atherosclerosis.

Cited by 35 publications

References 19 publications

Peripheral differential leukocyte counts in humans vary with hyperlipidemia, smoking, and body mass index

Peripheral differential leukocyte counts in humans vary with hyperlipidemia, smoking, and body mass index

Role of leucocytes in damage to the vascular endothelium during ischaemia-reperfusion injury

The Edinburgh Artery Study: is lytic activity bad for the heart?

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