Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa

Emi, Kazuyuki; Kawakami, Kazuyoshi; Miyairi, Shinichi; Tanno, Hiromasa; Otomaru, Hirono; Hatanaka, Arina; Sato, Syozo; Hayashi, Daisuke; Shibuya, Nobuhito; Imai, Yoshimichi; Gotoh, Naomasa; Maruyama, Ryoko; Tachi, Masahiro

doi:10.1016/j.jdermsci.2013.01.004

Cited by 12 publications

(27 citation statements)

References 39 publications

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“…8) Seth et al also demonstrated that impaired host immunity allows bacteria to establish wound infection. 32) In contrast, Kanno et al reported accelerated wound healing following PAO-1 inoculation 33) or 3OC12-HSL administration, 34) thus supporting the latter situation. Future studies are needed to explore this hypothesis.…”

Section: Discussionmentioning

confidence: 95%

Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts

Nakagami

Minematsu

Morohoshi

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Quorum sensing is a cell-to-cell communication mechanism, which is responsible for regulating a number of bacterial virulence factors and biofilm maturation and therefore plays an important role for establishing wound infection. Quorum-sensing signals may induce inflammation and predispose wounds to infection by Pseudomonas aeruginosa; however, the interaction has not been well investigated. We examined the effects of the P. aeruginosa las quorum-sensing signal, N-3-oxo-dodecanoyl homoserine lactone (3OC12-HSL), on matrix metalloproteinase (MMP) 9 expression in Rat-1 fibroblasts. 3OC12-HSL upregulated the expression of the MMP9 gene bearing an activator protein-1 (AP-1) binding site in the promoter region. We further investigated the mechanism underlying this effect. c-Fos gene expression increased rapidly after exposure to 3OC12-HSL, and nuclear translocation of c-Fos protein was observed; both effects were reduced by pretreatment with an AP-1 inhibitor. These results suggest that 3OC12-HSL can alter MMP9 gene expression in fibroblasts via the AP-1 signaling pathway.

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Section: Discussionmentioning

confidence: 95%

Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts

Nakagami

Minematsu

Morohoshi

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…The cytokines TNF-α, IL-18 and IL-8 are pro-inflammatory cytokines that contribute to the pathology of inflammatory skin disorders, whilst IL-10 on the other hand, is an immunosuppressive cytokine capable of down-regulating inflammation (Numerof and Asadullah, 2006;Noh and Lee, 2012). TNF-α, IL-18 and IL-8 have also been shown to play a role in wound healing (Kanno et al, 2013;Kampfer et al, 2000;Jiang, Sanders, Ruge & Harding, 2012). An investigation into the ability of honey to modulate the production of such cytokines by skin cells would help determine if honey has immunosuppressive, immune stimulatory or wound healing properties that may contribute to skin healing.…”

Section: Discussionmentioning

confidence: 99%

Honey: an immunomodulatory agent for disorders of the skin

McLoone

Warnock

Fyfe

2015

Food and Agricultural Immunology

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Studies have shown that honeys from around the world can inhibit the growth of a range of dermatologically important microbes. As well as reports of the antimicrobial properties of honey, a number of recent in vitro and in vivo studies suggest that honey is able to modulate immunological parameters related to the skin immune system. Paradoxically, both immunestimulatory and anti-inflammatory effects have been observed. In this review, scientific research investigating the immunomodulatory properties of honeys from around the world, in relation to disorders of the skin, is evaluated. Whilst there is sufficient evidence to suggest that honey does indeed have immunomodulatory properties, which may at least partially explain the ability of honey to promote the healing of wounds, there are still gaps in the scientific knowledge and literature. More research is necessary for a more complete understanding of the immune modulating properties of honey and to enable the utilisation of honey as an immune-modulating agent in dermatology.2

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“…3-Oxo-C12-HSL is synthesized by an autoinducer synthase in Pseudomonas (P.) aeruginosa, which regulates the production of virulence factors in this bacterium. Inoculation of P. aeruginosa strain significantly accelerated wound healing and inflammatory response in SpragueDawley rats [27]. These responses were reversed by inoculation with treatment with anti-3-oxo-C12-HSL antiserum.…”

Section: Miscellaneous Factorsmentioning

confidence: 95%