Neutrophil Degranulation Activity in Preeclampsia

Amagase, Hironobu; Ishida, Kiyoshi; Koyanagi, Takashi; Tsukimori, Kiyomi; Nakano, Hitoo

doi:10.3109/10641959709031637

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…19 Studies on circulating neu trophils demonstrate that neutrophil degranulation is already activated in preeclampsia, with a de crease in the total granule content retained in the neutrophil. 20 This activation could have occurred in the placental bed prior to returning to the mater nal circulation. T and B cells may also be involved.…”

Section: The Maternal Immunological Responsementioning

confidence: 99%

Antioxidants and Inflammatory Cell Response in Preeclampsia

Walker

1998

Semin Reprod Med

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There is widespread evidence of inflammatory cell and antioxidant activity in preeclampsia. However, it is difficult to disentangle the pathological changes from the normal physiological responses to the pathological process. The site at which the measurements are taken, and the severity of disease, alter the results. The interaction between the mother and the fetus needs to be considered separately, especially when the genetics of preeclampsia is considered. It is clear that within the placenta, there is an increase in tumor necrosis factor-alpha (TNF-alpha) and lipid peroxide production. These changes are associated with a reduction in the various placental antioxidants. This suggests there may be a failure of the normal fetal protection systems. Lipid peroxidation is also increased in the peripheral blood, as well as IL-6, IL-8, and TNF-alpha, which are of monocytic origin. Stimulated monocytes produce free radicals, which can cause oxidative damage. Maternal cells protect themselves with both plasma and intracellular antioxidants. There is an imbalance between oxidant and antioxidant activity in preeclampsia. Changes in membrane oxidation can lead to changes in the membrane stability. Genetic difference in the production of TNF-alpha and nitric oxide may also modify the disease process, demonstrating the role for "moderator genes."

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Section: The Maternal Immunological Responsementioning

confidence: 99%

Antioxidants and Inflammatory Cell Response in Preeclampsia

Walker

1998

Semin Reprod Med

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“…For instance, studies using luminol chemilu‐minescence have shown a trend for increased respiratory burst activity during pregnancy 24 , while those employing more selective measurements of superoxide anions, such as lucigenin, have shown a consistent reduction 25 . More recently, Tsulumori et al 26 reported an increase in the respiratory burst of neutrophils during pre‐eclampsia using the superoxide dismutase‐inhibitable reduction of femcytochrome C. Although employing a direct measurement of superoxide anion release‐similar to that of lucigenin chemiluminescence‐this study could not be repeated or substantiated in preliminary studies by Ama‐gase el at 27 …”

Section: Discussionmentioning

confidence: 80%

Neutrophil function in women with pre‐eclampsia

Crocker

Wellings

Fletcher

et al. 1999

BJOG

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ObjectiveDesign Baseline levels and activated responses of peripheral blood neutrophils were measured in Sample Neutrophils of 16 pre-eclamptic, 17 normal pregnant (third trimester) and 15 nonpregnant ageSetting Antenatal Clinic, City Hospital, Nottingham. MethodsTo investigate the function of neutrophils in normal pregnancy and pre-eclampsia.response to the physiological agonists, n-formyl-met-leu-phe (fMLP) and zymosan activated serum.matched control women were calculated.Neutrophil superoxide anion production was determined by lucigenin-enhanced chemiluminescence; the release of secondary granule lactoferrin by ELISA; and the expression of cell surface adhesion molecules CD1 lb, CD18 and L-selectin (CD62L) by flow cytometric analysis.Superoxide anion generation was reduced in the pregnant group compared with nonpregnant controls [fMLP by 5 1 % ( P = 0.03) and zymosan activated serum by 56% (P = O.Ol)] but pre-eclamptic measurements did not show a similar reduction. There were no differences between the three study groups in the plasma levels of lactofenin, or in the stimulated expression and release of CD1 I b and CD18, or lactoferrin and L-selectin. The base-line levels for the production of superoxide anions; the expression of CD1 l b or CD18 or L-selectin; and the release of lactofemn showed no significant differences.Conclusions Circulating neutrophils in pregnancy and pre-eclampsia are neither activated nor primed in vivo, however the release of reactive oxygen species is diminished in normal pregnancy. In comparison, an elevation of reactive oxygen generation in pre-eclampsia may highlight a role for neutrophils in the oxidative stress and pathophysiology of this disease.

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“…However, the role of β-glucuronidase in the context of bovine pregnancy has been poorly elucidated and very limited literatures are available depicting its significant role during pregnancy. Amagase et al (1997) reported no significant difference in the β-glucuronidase activity in the neutrophils of the non-pregnant and the normal pregnant women. However, in contrast to this, we noted significantly reduced level of β-glucuronidase activity in the neutrophils obtained from the pregnant cows.…”

Section: Discussionmentioning

confidence: 91%

Indoleamine 2, 3-dioxygenase 1 mediated alterations in the functionality of immune cells, decipher the pregnancy outcomes in crossbred dairy cows

Mohapatra

Chaudhary

Panda

et al. 2022

Preprint

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Pregnancy establishment in bovines requires maternal immune cell modulation. Present study investigated possible role of immunosuppressive indolamine-2, 3-dioxygenase 1 (IDO1) enzyme in the alteration of neutrophil (NEUT) and peripheral blood mononuclear cells (PBMCs) functionality of crossbred cows. Blood was collected from non-pregnant (NP) and pregnant (P) cows, followed by isolation of NEUT and PBMCs. Plasma pro-inflammatory (IFNγ and TNFα) and anti-inflammatory cytokines (IL-4 and IL-10) were estimated by ELISA and analysis of IDO1 gene in NEUT and PBMCs by RT-qPCR. Neutrophil functionality was assessed by chemotaxis, measuring activity of myeloperoxidase and β-D glucuronidase enzyme and evaluating nitric oxide production. Changes in PBMCs functionality was determined by transcriptional expression of pro-inflammatory (IFNγ, TNFα) and anti-inflammatory cytokine (IL-4, IL-10, TGFβ1) genes. Significantly elevated (P < 0.05) anti-inflammatory cytokines, increased IDO1 expression, reduced NEUT velocity, MPO activity and NO production observed only in P cows. Significantly higher (P < 0.05) expression of anti-inflammatory cytokines and TNFα genes were observed in PBMCs. Study highlights possible role of IDO1 in modulating the immune cell and cytokine activity during early pregnancy and may be targeted as early pregnancy biomarkers.

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Neutrophil Degranulation Activity in Preeclampsia

Cited by 6 publications

References 18 publications

Antioxidants and Inflammatory Cell Response in Preeclampsia

Antioxidants and Inflammatory Cell Response in Preeclampsia

Neutrophil function in women with pre‐eclampsia

Indoleamine 2, 3-dioxygenase 1 mediated alterations in the functionality of immune cells, decipher the pregnancy outcomes in crossbred dairy cows

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