Neutrophil cytoplasmic antibodies (p-ANCA) in ulcerative colitis.

Ellerbroek, Pauline M.; Pool, M. Oudkerk; Ridwan, Ben U.; Dolman, Koert M.; Blomberg, B. Mary E. von; Borne, A. E. G. K. Von Dem; Meuwissen, S. G. M.; Goldschmeding, Roel

doi:10.1136/jcp.47.3.257

Cited by 53 publications

(26 citation statements)

References 24 publications

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“…Granulocytes were obtained from fresh human blood using LymphoprepÔ (Nycomed Pharma AS, Oslo, Norway) density gradient centrifugation and further isolated as described by Ellerbroek et al 27 Freshly isolated granulocytes were used for further experiments as described next. The isolated granulocytes were CD15 1 and CD45 low + as determined by flow cytometric analysis.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Broek

Kroeze

Waaijman

et al. 2014

Tissue Engineering Part A

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Many cell-based regenerative medicine strategies toward tissue-engineered constructs are currently being explored. Cell-cell interactions and interactions with different biomaterials are extensively investigated, whereas very few studies address how cultured cells will interact with soluble wound-healing mediators that are present within the wound bed after transplantation. The aim of this study was to determine how adipose tissue-derived mesenchymal stem cells (ASC), dermal fibroblasts, and keratinocytes will react when they come in contact with the deep cutaneous burn wound bed. Burn wound exudates isolated from deep burn wounds were found to contain many cytokines, including chemokines and growth factors related to inflammation and wound healing. Seventeen mediators were identified by ELISA (concentration range 0.0006-9 ng/mg total protein), including the skin-specific chemokine CCL27. Burn wound exudates activated both ASC and dermal fibroblasts, but not keratinocytes, to increase secretion of CXCL1, CXCL8, CCL2, and CCL20. Notably, ASC but not fibroblasts or keratinocytes showed significant increased secretion of vascular endothelial growth factor (5-fold) and interleukin-6 (253-fold), although when the cells were incorporated in bi-layered skin substitute (SS) these differences were less pronounced. A similar discrepancy between ASC and dermal fibroblast mono-cultures was observed when recombinant human-CCL27 was used instead of burn wound exudates. Although CCL27 did not stimulate the secretion of any of the wound-healing mediators by keratinocytes, these cells, in contrast to ASC or dermal fibroblasts, showed increased proliferation and migration. Taken together, these results indicate that on transplantation, keratinocytes are primarily activated to promote wound closure. In contrast, dermal fibroblasts and, in particular, ASC respond vigorously to factors present in the wound bed, leading to increased secretion of angiogenesis/granulation tissue formation factors. Our findings have implications for the choice of cell type (ASC or dermal fibroblast) to be used in regenerative medicine strategies and indicate the importance of taking into account interactions with the wound bed when developing advanced therapies for difficult-to-close cutaneous wounds.

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Section: Cell Isolation and Culturementioning

confidence: 99%

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Broek

Kroeze

Waaijman

et al. 2014

Tissue Engineering Part A

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“…As yet, the antigens that stimulate p-ANCA production are poorly defined. Nonetheless, evidence suggests that lactoferrin (but not myeloperoxidase [7,29]) may be a major target antigen for the p-ANCA of inflammatory bowel disease [27][28][29][30][31]. Walmsley et al [32] detected serum anti-lactoferrin antibodies in only 2 of 52 patients with ulcerative colitis, but in another study, immunoglobulin G anti-lactoferrin antibodies were found in the sera from 50 % of patients with ulcerative colitis and\or PSC [29].…”

Section: Discussionmentioning

confidence: 92%

“…Indeed, high plasma lactoferrin concentrations have been reported in active rheumatoid arthritis and systemic lupus erythematosus [60] -conditions also associated with the presence of serum anti-neutrophil antibodies [61,62]. In inflammatory bowel disease, approximately 22-89 % of patients with ulcerative colitis [4,7,28,29,[31][32][33][34][35][36][37][38] and up to 34 % of patients with Crohn's disease [4,28,[32][33][34]36,41,63] are p-ANCA positive. As yet, the antigens that stimulate p-ANCA production are poorly defined.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, lactoferrin is one of the antigens for perinuclear staining anti-neutrophil cytoplasmic antibodies (p-ANCA) [26][27][28][29][30][31][32]. These antibodies have been reported in the sera of 13-89 % of patients with inflammatory bowel disease [3,7,29,[31][32][33][34][35][36][37][38] and 65-82 % of patients with PSC [4,29,36,[39][40][41].…”

Section: Introductionmentioning

confidence: 99%

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Biliary lactoferrin concentrations are increased in active inflammatory bowel disease: a factor in the pathogenesis of primary sclerosing cholangitis?

et al. 1998

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1. One hypothesis for the link between inflammatory bowel disease and primary sclerosing cholangitis is that neutrophil activators, such as bacterial chemotactic peptides or neutrophil granule products themselves, pass from the inflamed colon to the liver via an enterohepatic circulation. However, there are no data on biliary concentrations of neutrophil granule products in patients with active and inactive inflammatory bowel disease.2. Gall bladder bile was obtained at laparotomy from 42 patients with ulcerative colitis and 21 patients with Crohn's disease. Biliary lactoferrin and myeloperoxidase concentrations were quantified by ELISA.3. In active ulcerative colitis, the mean lactoferrin concentration in gall bladder bile of 2.8+/-0.40 mg/l was higher than that seen after colectomy (1.2+/-0.11 mg/l; P<0.0001) or in patients with pouchitis (1.8+/-0.34 mg/l; P=0.06). In active Crohn's colitis, the mean lactoferrin concentration was 3.7+/-0.9 mg/l, compared with 1.1+/-0. 24 mg/l in the post-colectomy group (P<0.05) and 3.1+/-0.71 mg/l in those with active ileitis or ileocolitis. In contrast, biliary myeloperoxidase concentrations were low and comparable in all groups, with a mean concentration in the 42 patients with ulcerative colitis of 11.2+/-1.9 microgram/l.4. In contrast to myeloperoxidase, biliary lactoferrin concentrations are increased in active ulcerative colitis and Crohn's disease, and fall with colectomy and with disease remission. These findings indirectly support the hypothesis that bacterial chemotactic peptides (which induce selective degranulation of neutrophil secondary granules), and/or lactoferrin itself, undergo an enterohepatic circulation.

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“…1,2 Inflammatory bowel conditions are associated with rare atypical P-ANCAs, against cathepsin G, elastase, and bacterial permeability increasing proteins. 9,10 The Case 1 photomicrograph (Figure 1b) perhaps illustrates an atypical P-ANCA, which could be due to the presence of atypical antigens. Recently, ulcerative colitis patients have been described to have PR3-ANCA.…”

Section: Discussionmentioning

confidence: 99%

Antineutrophil cytoplasmic antibodies: two cases of changing antigenic specificity

Saleem¹,

Oliver

Rowbottom³

et al. 2012

Ann Clin Biochem

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The transformation of the antineutrophil cytoplasmic antibody (ANCA) specificity in the absence of specific drug treatment has not been reported in the literature. A few studies have suggested changes in the epitopes recognized by the ANCAs. We describe two patients who switched from myeloperoxidase-positive to PR3 ( proteinase 3)-positive ANCA during the course of their disease process and subsequently remained unchanged. One patient developed ulcerative colitis following the appearance of PR3-ANCA while the other remains quiescent. Regular follow-up and close monitoring of ANCA specificity are essential. A change of specificity may indicate the development of a new ANCA-related disease.

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Neutrophil cytoplasmic antibodies (p-ANCA) in ulcerative colitis.

Cited by 53 publications

References 24 publications

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Biliary lactoferrin concentrations are increased in active inflammatory bowel disease: a factor in the pathogenesis of primary sclerosing cholangitis?

Antineutrophil cytoplasmic antibodies: two cases of changing antigenic specificity

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