Neutrophil cytochemistry in bacterial infection.

Mackie, P H; Mistry, D K; Wozniak, Janina; Dodds, W N; Geddes, A M; Stuart, J.

doi:10.1136/jcp.32.1.26

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its early detection is difficult, however, since clinical signs are often masked by neutropenia, and bacteriological culture usually requires 24 hours. Rapid screening tests for infection, such as neutrophil cytochemistry or the Limulus test for endotoxaemia, have not yet proved sufficiently reliable when applied to the individual patient (Martinez-G et al, 1973, Steigbigel et al, 1974Mackie et al, 1979).…”

mentioning

confidence: 99%

C-reactive protein for rapid diagnosis of infection in leukaemia.

Mackie¹,

Crockson²,

Stuart³

1979

J Clin Pathol

Self Cite

View full text Add to dashboard Cite

C-reactive protein, measured in serum from 38 patients with leukaemia, was elevated to at least 100 mg/l at the beginning of 32 of 34 episodes of infection, and subsequently rose above 100 mg/l in all 34. Uninfected patients, whether in leukaemic remission or relapse and whether pyrexial or not, always had levels below 100 mg/l, with four exceptions out of 290 measurements. Estimation of two other acute-phase proteins, alpha 1-antitrypsin and orosomucoid, was not of additional diagnostic value. Serial measurement of C-reactive protein may be important for the early detection of infection in the leukaemic patient with neutropenia.

show abstract

mentioning

confidence: 99%

C-reactive protein for rapid diagnosis of infection in leukaemia.

Mackie¹,

Crockson²,

Stuart³

1979

J Clin Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rather than indicating the presence of one particular enzyme, the cellular reaction to CAE represents the activity of multiple esterase enzymes (Li et al 1973). Increased stain reaction to CAE has been noted in studies of human patients with local and systemic bacterial infections (Wozniak et al 1978;Mackie et al 1979). The increased stain uptake observed in the neutrophils of these patients was believed to be due to an increase in membrane permeability after exposure to bacterial products.…”

Section: Discussionmentioning

confidence: 94%

Cytochemistry of leukocytes from the family Macropodidae

Hulme-Moir

Clark²

2010

Comp Clin Pathol

View full text Add to dashboard Cite

The cytochemical staining characteristics of leukocytes from four species of Macropodidae and one species of Potoroidae were studied to investigate the cellular composition of leukocytes within the Macropodidae family and determine markers that may be useful for identifying cell lineage. Blood smears from the tammar wallaby (Macropus eugenii), the western grey kangaroo (Macropus fuliginosis), the red kangaroo (Macropus rufus), quokka (Setonix brachyurus) and a potoroid species, the woylie (Bettongia pencillata) were examined following reaction for Sudan Black B (SBB), peroxidase (PER), chloracetate esterase (CAE), α-naphthyl butyrate esterase (NBE) and alkaline phosphatase (ALP). Similar to domestic animal species, the neutrophils and eosinophils of macropodid species stained for both SBB and PER while monocytes and lymphocytes showed little or no reaction. CAE and NBE, however, were not useful as markers for macropodid neutrophils and monocytes, respectively. Significant variation between species was seen in ALP content.Tammar wallabies and quokkas demonstrated strong ALP activity within their neutrophils and eosinophils. In contrast, western grey kangaroos and red kangaroos contained no ALP activity in any of their leukocytes and staining in woylies was limited to weak reaction within some eosinophils.

show abstract

Use of chloroacetate esterase staining for the histological diagnosis of prosthetic joint infection

et al. 2015

View full text Add to dashboard Cite

A heavy neutrophil polymorph infiltrate [>5 per high-power field (HPF) after examination of at least 5 HPF by Musculoskeletal Infection Society (MSIS) criteria] is characteristically seen in peri-implant tissues of infected prosthetic hip and knee joints. We determined whether chloroacetate esterase (CAE) staining facilitated the identification of neutrophil polymorphs in peri-implant tissues in cases of hip and knee arthroplasty infection and reassessed MSIS criteria in the light of our findings. Frozen and paraffin sections of peri-prosthetic tissues of 76 cases of failed hip and knee arthroplasties classified as septic or aseptic loosening microbiologically were analysed histologically by both haematoxylin-eosin and CAE staining. The extent of the neutrophil polymorph infiltrate was determined semiquantitatively and correlated with the microbiological and clinical diagnosis. CAE staining facilitated identification of neutrophil polymorphs in arthroplasty tissues. All cases of aseptic loosening contained fewer than two neutrophil polymorphs per HPF. CAE staining showed that in some cases of septic loosening, fewer than five neutrophil polymorphs per HPF (on average) are present in peri-prosthetic tissues. The histological criterion of more than two neutrophil polymorphs per HPF showed increased sensitivity and accuracy for the diagnosis of septic loosening. CAE is a useful stain that facilitates the identification of neutrophil polymorphs in both frozen and paraffin sections of peri-implant tissues. CAE staining shows that some microbiologically confirmed cases of septic loosening contain relatively few neutrophil polymorphs, indicating that the MSIS histological criterion of more than five neutrophil polymorphs per HPF is too high an index figure for the diagnosis of all cases of hip and knee arthroplasty infection.

show abstract

Neutrophil cytochemistry in bacterial infection.

Cited by 4 publications

References 15 publications

C-reactive protein for rapid diagnosis of infection in leukaemia.

C-reactive protein for rapid diagnosis of infection in leukaemia.

Cytochemistry of leukocytes from the family Macropodidae

Use of chloroacetate esterase staining for the histological diagnosis of prosthetic joint infection

Contact Info

Product

Resources

About