Neutrophil chemotactic substances in different fractions of soluble dental plaque material

Helldén, Leif; Ericson, Thomas; Lindhe, Jan

doi:10.1111/j.1600-0722.1973.tb00593.x

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…This observation corroborates results previously reported by e.g. Tempel et al (1970), Miller, Umana & Folke (1971, Lindhe & Hellden (1972), Hellden, Ericsson & Lindhe (1973), Kraal & Loesche (1974) describing that material obtained from dental plaque or from Actinomyces and Streptococcus strains was consistently active for neutrophil chemotaxis when tested both in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 93%

“…Tempel et al (1970) isolated a small molecular weight peptide from pure cultures of a hamster strain of A. viscosus with chemotactic activity. Similar properties could be demonstrated directly in supernatants of human dental plaque with peak activities in small molecular weight fractions (Hellden, Ericsson & Lindhe 1973). Chemotactic factors have not been sought in cultures of Gram-negative plaque bacteria.…”

Section: Discussionmentioning

confidence: 86%

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Chemotaxis and vascular permeability produced by human periodontopathic bacteria

Lindhe

Socransky

1979

J of Periodontal Research

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The present investigation was carried out in an attempt to assess the chemotactic and vascular permeability inducing potential of some microorganisms which have been associated with gingivitis, advanced periodontitis and periodontosis. The four microorganisms studied in the present experiment, T. denticola, A. viscosus, B. melaninogenicus and C. ochraceus were isolated from human periodontal disease. Bacterial samples were removed by means of an oxygen-free gas flushed syringe from specific sites within gingival pockets of humans displaying signs of varying degrees of gingivitis, rapidly advancing periodontitis or periodontosis. The bacterial cells were harvested from respective growth media by centrifugation, washed and sonicated. After lysis the resulting suspension was lyophilized. Prior to test the different lyophilized sonicates were dissolved in saline and the protein concentration of the solutions adjusted to 10 wg/ml. The chemotactic activity elaborated by the different bacterial test materials was studied by an in vitro technique originally described by Boyden (1962). Measurements of vascular exudation were performed in wound chambers implanted subcutaneously in the back of rats. The results demonstrated that preparations obtained from microorganisms present in human dental plaques contain components which are chemotactic for neutrophil leukocytes and are capable of inducing or mediating increased vascular leakage in a granulation tissue. Material from Capnocytophaga ochraceus in comparison to substances from T. denticola, A. viscosus and B. melaninogenicus proved to be rather inactive in promoting neutrophil chemotaxis and vascular exudation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 86%

Chemotaxis and vascular permeability produced by human periodontopathic bacteria

Lindhe

Socransky

1979

J of Periodontal Research

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“…When different fractions were tested regarding their chemotactic activity it was found that all the plaque fractions tested attracted mononuclear leukocytes. The findings were thus in line with earlier published data concerning the capacity of dental plaque to attract neutrophilic Ieukoc34es (Tenapel et al 1970, Miller et al 1971, Lindhe & Helldeo 1972, Hellden, Ericson & Lindhe 1973. However, the molecular size of the plaqne fraction that induced the major part of the inonoc3fte chemotactic activity was larger than that of the corresponding neutrophil chemotactic factor .…”

Section: Discussionsupporting

confidence: 91%

Monocyte chemotactic activity in human dental plaque

Helldén

1977

J of Periodontal Research

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The monocyte chemotactic activity elaborated by soluble dental plaque material and by various fractions of the plaque material was studied in vitro with the modified Boyden chamber technique. Monocytes were harvested from the peritoneal cavity of rabbits after intraperitoneal injection of a 1 % suspension of hydrolysed starch. The plaque material was separated in Sephadex gel columns (G‐25 and G‐20Q). Three fractions differing from each other in molecular size were selected from the protein peaks in the chromatograms. The results showed that dental plaque material contains factors with a chemotactic effect on monocytes. Examination of various fractions of dental plaque material revealed that the major part of the monocyte chemotactic activity resided in a fraction with an appoximate molecular size of 10,000–70,000.

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“…Furthermore, plaque accumulation will be inhibited by chiorhexidine, and this should greatly increase the tendency toward healing. In fact, the application of plaque extract led to a more severe inflammatory reaction within granulation tissue when compared to chlorhexidine (Lindhe & Hellden 1972, Hellden et al 1973. The positive influence of plaque inhibition on wound healing has also been demonstrated by Hamp et al, (1975) in his histoiogically verified studies of the epithelial attachment in dogs, though these results are representative of only the especially contaminated gingival area.…”

Section: Discussionmentioning

confidence: 91%

Influence of chlorhexidine rinsing on the healing of oral mucosa and osseous lesions

Bassetti

Kallenberger

1980

J Clinic Periodontology

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The influence of chlorhexidine rinsing on wound healing was studied using standardized, open mucosal-osseous wounds in the left side of the palate in Wistar rats. In five test groups, each containing 10 rats, rinsing was performed twice daily for 30 sec with 0.1, 0.2 and 0.5% chlorhexidine solution, chlorhexidine solution vehicle, and Ringer solution. A sixth test group (control) was not rinsed at all. Seven days postoperatively, wound healing was evaluated clinically (size of the defect) and histomorphometrically (percent composition of mature connective tissue, immature connective tissue, granulation tissue, fibrin with granulocytic infiltrate). Clinically it was clear that wound healing was best in those animals that rinsed with Ringer solution, and worst in those that rinsed with 0.5% chlorhexidine solution. Increasing concentration of chlorhexidine caused a delay in wound healing, which in the following cases resulted in significant differences: rinsing with Ringer solution and vehicle versus all concentrations of chlorhexidine, no rinsing versus 0.5% chlorhexidine. Intensive rinsing with high concentrations of chlorhexidine may, after oral surgical operations, especially surgery in which bone is exposed, result in delay and disturbance of wound healing in humans.

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Neutrophil chemotactic substances in different fractions of soluble dental plaque material

Cited by 8 publications

References 11 publications

Chemotaxis and vascular permeability produced by human periodontopathic bacteria

Chemotaxis and vascular permeability produced by human periodontopathic bacteria

Monocyte chemotactic activity in human dental plaque

Influence of chlorhexidine rinsing on the healing of oral mucosa and osseous lesions

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