Neutrophil chemokines in cultured nasal fibroblasts

Rudack, Claudia; Hermann, W.; Eble, Johannes A.; Schroeder, Johann O.

doi:10.1034/j.1398-9995.2002.23748.x

Cited by 13 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its protein level and biological potency were higher than GRO‐ α > GCP‐2 > ENA‐78 following RP‐8 chromatography. Both IL‐8 and GRO‐ α are known to be synthesized by primary nasal epithelial cells, nasal fibroblasts and neutrophils in response to various stimuli in vitro [25,26]. In vitro , IL‐1 β and tumour necrosis factor (TNF)‐ α seem to be potent stimulators of chemokine production, whereas interferon (IFN)‐ γ inhibits chemokine production [18].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the weaker biological potency of GCP‐2 and the smaller amounts of GCP‐2 secreted, compared with those of IL‐8, GCP‐2 was co‐produced with IL‐8 and GRO‐ α in peritonsillar abscesses. It has been suggested that GCP‐2 is a typical mesenchymal cell‐derived chemokine as shown in stimulation experiments within nasal fibroblasts, whereas IL‐8 is also released by blood cells, especially neutrophils [18,26].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biologically active neutrophil chemokine pattern in tonsillitis

Rudack

Jörg

Sachse

2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYTo gain an insight into the mechanisms of chronic and acute inflammation, the production of neutrophil chemokines in different types of tonsillitis -hyperplastic tonsillitis (HT), recurrent tonsillitis (RT) and peritonsillar abscesses (PA) -was investigated. The chemokines interleukin-8 (IL-8), growth-related oncogene-a (GRO-a ), epithelial cell-derived neutrophil attractant-78 (ENA-78) and granulocyte chemotactic protein-2 (GCP-2) were detected and shown to have different biological activities. With respect to the biological properties of CXC chemokines, the biological activity of the chemokines was identified using a three-step high-performance liquid chromatography (HPLC) technique, a bioassay involving measurement of neutrophil chemotaxis in a single Boyden chamber in tissue of HT, RT and PA. Using reverse transcription-polymerase chain reaction (RT-PCR), the chemokine concentrations were determined in the different tonsillitis entities. The chemokine pattern was dominated in PA by IL-8 and GRO-a and in RT by GRO-a . Hyperplastic tonsils of patients without a history of infection generated about five times lower IL-8 than PA. A protein concentration of GCP-2 was induced in PA and RT, whereas ENA-78 remained the same in all entities. In conclusion, it would appear that IL-8 was upregulated in acute inflammation, whereas GRO-a dominated in chronic inflammation. ENA-78 seems not to play a pivotal role in inflammatory processes in tonsils. GCP-2 may serve as a substitute chemokine in certain inflammatory conditions as its quantity of mRNA and protein was higher in RT and PA than in HT.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biologically active neutrophil chemokine pattern in tonsillitis

Rudack

Jörg

Sachse

2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…This inflammation is marked by an infiltration of neutrophils, recruited by tissue debris or fibroblasts (19). This neutrophilic inflammation predominates during the first hours with a simultaneous release of proteases.…”

Section: Model Again: Wound Repair After Sinus Surgerymentioning

confidence: 99%

Tissue remodelling in upper airways: where is the link with lower airway remodelling?

Watelet

Zele

Gjomarkaj

et al. 2006

Allergy

View full text Add to dashboard Cite

Tissue remodelling reported in upper airways include epithelial hyperplasia, increased matrix deposition in the nasal or paranasal lining, matrix degradation and accumulation of plasma proteins. Genetic influences, foetal exposures and early life events may contribute to structural changes such as subepithelial fibrosis from an early age. Other structural alterations are related to duration of the disease and long-term uncontrolled inflammation. Structural changes may increase alteration of the protective functions of the upper airways namely by affecting mucociliary clearance and conditioning of inspired air. The sequences of tissue changes during wound repair of upper airway mucosa after surgery are illustrative of the complexicity of tissue modelling and remodelling and could be considered as an important source of information for a better understanding of the complex relationship between inflammatory reaction, of the subsequent tissue damages and fibroblast metabolism of upper airways

show abstract

“…As a result, the biological activities of IL-8 on neutrophils and the mechanism of signal transduction have been studied in great detail, and their effects have been compared with those of well-characterized neutrophil attractants such as C5a, fMet-Leu-Phe, platelet activating factor (PAF), and leukotriene (LT) B4. 4,5) The observation that IL-8 is a product of monocytes and macrophages and is also generated by tissue cells, was very important to understanding of the numerous potential implications of this chemokine in inflammatory conditions. IL-8 is associated with rhinitis, 6) bronchitis, 7) pulmonary fibrosis, 8) psoriasis, 9) inflammatory bowel disease, 10) and other disorders.…”

mentioning

confidence: 99%

Inhibition of IL-8 Production by Green Tea Polyphenols in Human Nasal Fibroblasts and A549 Epithelial Cells

Kim

Sun

et al. 2006

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The attraction of leukocytes to tissues is essential in order for inflammation and the host response to infection to occur. The process is controlled by chemokines, a term which refer to chemotactic cytokines. 1,2) In their monomeric forms, chemokines are 8-to 10-kD proteins with 20 to 70% homology in amino acid sequences, and are characteristically basic heparin-binding proteins, which facilitate binding to cells and matrix components. There are at least four chemokine families, but only two have been extensively characterized. These two subfamilies, which contain four cyteines, appear to be the largest groups. They can be distinguished according to the position of first two cysteines, which either are separated by one amino acid (CXC chemokine) or are adjacent to each other (CC chemokines). The CXC chemokines can be further subdivided into those that contain the sequence glutamic acid-leucine-arginine (ERL) near the N-terminal, and those that do not.3) The CXC chemokines that contain the sequence are chemotactic for neutrophils, whereas those that do not contain in the sequence act on lymphocytes.Airway inflammation is a very common cause of illness with a substantial impact on health care. Neutrophils play an essential role in the host defense and in inflammation, but the latter may trigger and sustain the pathogenesis of a range of acute and chronic diseases. Infiltration of neutrophils occurs as a response to chemoattractant molecules by resident tissue cells. The recruitment of neutrophils in airway inflammation may account for the generation of interleukin 8 (IL-8), the prototypic CXC chemokine. IL-8 was identified as an agonist for neutrophils on the basis of two in vitro effects chemotaxis and the release of granule enzymes. As a result, the biological activities of IL-8 on neutrophils and the mechanism of signal transduction have been studied in great detail, and their effects have been compared with those of well-characterized neutrophil attractants such as C5a, fMet-Leu-Phe, platelet activating factor (PAF), and leukotriene (LT) B4. 4,5) The observation that IL-8 is a product of monocytes and macrophages and is also generated by tissue cells, was very important to understanding of the numerous potential implications of this chemokine in inflammatory conditions. IL-8 is associated with rhinitis, 6) bronchitis, 7) pulmonary fibrosis, 8) psoriasis, 9) inflammatory bowel disease, 10) and other disorders. Several other chemokines, such as growth-related oncogene (GRO)-a and ENA-78, are also potent neutrophil chemoattractants; this property depends on the presence of ERL motifs.2)The promoter of the IL-8 gene contains a binding site for the redox-responsive transcription factors, NF-kB, which have been shown to be important for IL-8 expression. 11,12) Activation of nuclear factor-kB (NF-kB) acts as a master switch or control point for the expression of a large number of proinflammatory genes.13) NF-kB, a member of the Rel family of transcription factors, is usually present in the cytoplasm of the cell in a...

show abstract

Neutrophil chemokines in cultured nasal fibroblasts

Abstract: Induction of IL-8 protein dominates chemokine synthesis 24 and 72 h after stimulation, whereas induction of GCP-2 mRNA seems to have a role in the early phase after 2 h of exposition with TNF-alpha.

Cited by 13 publications

References 32 publications

Biologically active neutrophil chemokine pattern in tonsillitis

Biologically active neutrophil chemokine pattern in tonsillitis

Tissue remodelling in upper airways: where is the link with lower airway remodelling?

Inhibition of IL-8 Production by Green Tea Polyphenols in Human Nasal Fibroblasts and A549 Epithelial Cells

Contact Info

Product

Resources

About