Neutrophil CD64 expression is not a useful biomarker for detecting serious bacterial infections in febrile children at the emergency department

Veen, Mirjam van; Nijman, Ruud; Zijlstra, Marieke; Dik, Willem A.; Rijke, Yolanda B. de; Moll, Henriëtte A.; Neele, Marjolein; Smit, Frank J; Oostenbrink, Rianne

doi:10.3109/23744235.2015.1118156

Cited by 4 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many potential novel biomarkers have been studied in children at risk of IBI, however most do not demonstrate better diagnostic accuracy than the traditionally used biomarkers of CRP and PCT [ 31 ]. Some novel markers show potential use in certain settings but a more limited role in others; for example, CD64 (a protein expressed on neutrophils) has been found to be a good diagnostic marker in neonatal sepsis but performed poorly in studies of febrile children [ 32 , 33 ]. This highlights the importance of conducting validation studies in a wide range of settings.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of mid-regional pro-Adrenomedullin as a biomarker of invasive bacterial infection in children: a systematic review

et al. 2022

View full text Add to dashboard Cite

Background Invasive bacterial infections (IBI) in children present a difficult clinical challenge. They are often life-threatening, however in the early stages they can be hard to differentiate from benign viral infections. This leaves clinicians with the risk of missing a serious IBI diagnosis or inappropriately using antimicrobials in a child with a viral infection- contributing to the ongoing development of increased antimicrobial resistance. Hence, biomarkers which could aid in early detection of IBI and differentiation from viral infections are desirable. Mid-Regional pro-Adrenomedullin (MR-proADM) is a biomarker which has been associated with IBI. The aim of this systematic review was to determine its diagnostic accuracy in identifying children with IBI. Methods A strategy was devised to search online databases MEDLINE, Embase, Web of Science and Scopus for human clinical trials reporting the accuracy of MR-proADM in children. Against predesigned inclusion and exclusion criteria full texts were selected for inclusion and data extraction. True positives, false positives, true negatives and false negatives were extracted from each included study to fill 2 × 2 tables. Using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool methodological quality of each study was assessed. Results A total of 501 articles were initially identified. After the removal of duplicates and abstract screening 11 texts were fully reviewed and four texts (totaling 1404 patients) were included in the systematic analysis. Only one study was of a high quality and that study accounted for the vast majority of patients. A single study reported the diagnostic accuracy of MR-proADM for invasive bacterial infection reporting an Area under the Curve of 0.69. The paucity of available studies made meta-analysis and studies of heterogeneity impossible. Conclusion There is a paucity of research regarding the diagnostic accuracy of MR-proADM in the diagnosis of invasive bacterial infections in children. Initial results would suggest that MR-proADM testing alone is poor at identifying IBI in young children. It remains unclear if MR-proADM performs differently in older children or in children with signs and symptoms of IBI. Trial registration PROSPERO CRD42018096295.

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnostic value of mid-regional pro-Adrenomedullin as a biomarker of invasive bacterial infection in children: a systematic review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…2,6 CD64 is an Fc receptor of immunoglobulin G (IgG) 7 that constitutively presents on the surface of monocytes and appears and increases in granulocytes in case of infection, systemic inflammatory response syndrome, or tissue damage. As a result, CD64 surface expression on granulocytes has been described as a useful biomarker for bacterial infection diagnosis in previously healthy patients, postoperative circumstances, [8][9][10] and the neonatal period. [10][11][12] The primary aim of our pilot study was to assess the surface expression levels of CD64 on monocytes and granulocytes in a cohort of patients with SAB admitted to a PICU.…”

Section: Introductionmentioning

confidence: 99%

CD64 on monocytes and granulocytes in severe acute bronchiolitis: Pilot study on its usefulness as a bacterial infection biomarker

García-Salido

Serrano-González

Casado‐Flores

et al. 2018

Journal of Leukocyte Biology

View full text Add to dashboard Cite

The CD64 receptor has been described as a biomarker of bacterial infection. We speculated that CD64 surface expression on monocytes and granulocytes of children with severe acute bronchiolitis (SAB) could be altered in cases of probable bacterial infection (PBI) determined using classical biomarkers (procalcitonin and C-reactive protein, leukocyte count, and radiographic findings). A prospective observational pilot study was conducted from October 2015 to February 2016 in children admitted for pediatric critical care. A blood sample was taken in the first 24 hours of admission, and CD64 was measured by flow cytometry. The values obtained were analyzed and correlated with traditional biomarkers of PBI. Thirty-two children were included; a correlation was found between CD64 expression and the PBI criteria. CD64 surface expression was higher in children with PBI (area under the receiver operating characteristic curve of 0.73; P = 0.042) and the percentage of CD64 granulocytes was higher in children with PBI. This is the first study to describe CD64 surface expression on monocytes and granulocytes in SAB, finding CD64 values to be higher in children with PBI. Larger clinical studies are needed to elucidate the real accuracy of CD64 as a biomarker of bacterial infection.

show abstract

Review and Updates on Pediatric Fever

Zheng

Glauser

2021

Curr Emerg Hosp Med Rep

View full text Add to dashboard Cite

Neutrophil CD64 expression is not a useful biomarker for detecting serious bacterial infections in febrile children at the emergency department

Abstract: NCD64 expression has poor discriminative value to detect children with an SBI in a general population of febrile children at the ED. It has no superior value compared to CRP in this setting, neither in total nor in sub-populations.

Cited by 4 publications

References 20 publications

Diagnostic value of mid-regional pro-Adrenomedullin as a biomarker of invasive bacterial infection in children: a systematic review

Diagnostic value of mid-regional pro-Adrenomedullin as a biomarker of invasive bacterial infection in children: a systematic review

CD64 on monocytes and granulocytes in severe acute bronchiolitis: Pilot study on its usefulness as a bacterial infection biomarker

Review and Updates on Pediatric Fever

Contact Info

Product

Resources

About