Neutrophil and Neutrophil-to-Lymphocyte Ratio as Clinically Predictive Risk Markers for Recurrent Pregnancy Loss

Jiang, Sushi; He, Fan; Gao, Rui; Chen, Chaogang; Zhong, Xiaozhu; Li, Xiaojia; Shen, Lin; Xu, Wenming; Qin, Lei; Zhao, Xiaomiao

doi:10.1007/s43032-020-00388-z

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…We then explored the relationship between clinical characteristics and immune cell populations. Our results showed that the peripheral neutrophils correlated positively with T cells, supporting previous reports that the neutrophil-to-lymphocyte ratio may be a clinically predictive risk marker for RPL ( 45 ). NKT-like cells could regulate the polarization of T cells and the secretion of proinflammatory cytokines ( 47 ).…”

Section: Discussionsupporting

confidence: 91%

“…Unlike other studies, our data showed the number of DCs (BDCA1 + , BDCA3 + , pDCs) in decidual tissue was low, and no difference between HDs and RPL patients, the underlying mechanism may be the small sample size in this study. Some researchers demonstrated that the increased neutrophile ratio may be a risk factor for RPL, but the exact mechanism still needs further investigation ( 45 ). Through our investigation, the frequency of neutrophils and their subtypes were no significant differences between RPL patients and HDs.…”

Section: Discussionmentioning

confidence: 99%

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The peripheral and decidual immune cell profiles in women with recurrent pregnancy loss

Qin

Xu²,

Chen³

et al. 2022

Front. Immunol.

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Recurrent pregnancy loss (RPL) affects 1-2% of couples of reproductive age. Immunological analysis of the immune status in RPL patients might contribute to the diagnosis and treatment of RPL. However, the exact immune cell composition in RPL patients is still unclear. Here, we used flow cytometry to investigate the immune cell profiles of peripheral blood and decidual tissue of women who experienced RPL. We divided peripheral immune cells into 14 major subgroups, and the percentages of T, natural killer T (NKT)-like and B cells in peripheral blood were increased in RPL patients. The decidual immune cells were classified into 14 major subpopulations and the percentages of decidual T, NKT-like cells and CD11chi Mφ were increased, while those of CD56hi decidual NK cells and CD11clo Mφ were decreased in RPL patients. The spearmen correlation analysis showed that the proportion of peripheral and decidual immune cells did not show significant correlations with occurrences of previous miscarriages. By using flow cytometry, we depicted the global peripheral and decidual immune landscape in RPL patients. The abnormalities of peripheral and decidual immune cells may be involved in RPL, but the correlations with the number of previous miscarriages need further verification.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The peripheral and decidual immune cell profiles in women with recurrent pregnancy loss

Qin

Xu²,

Chen³

et al. 2022

Front. Immunol.

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“…The main function of CD8+T cells is to recognize non-autogenic antigens and kill non-autogenic and infected cells, however, the role of CD8+T cells in the early trimester remains largely unknown. A recent study indicated that depletion of CD8+T cells, especially fetal antigen-specific CD8+T cells, is beneficial to the maintenance of maternal-fetal pregnancy 46 . The elevated level of effector memory CD8+T cells that do not express the inhibitory receptor PD1 was related to the occurrence of miscarriage and preeclampsia with a potential mechanism of immune activation and rejection of fetal antigen 47 .…”

Section: Discussionmentioning

confidence: 99%

Investigation and Validation of Molecular Characteristics of Endometrium in Recurrent Miscarriage and Unexplained Infertility from a Transcriptomic Perspective

Ran¹,

He²,

Chen³

et al. 2022

Int. J. Med. Sci.

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Recurrent miscarriage (RM) and unexplained infertility (UI) are gordian knots in reproductive medicine, which are troubling many patients, doctors, and researchers. Although these two diseases of early pregnancy have a significant impact on human reproductive health, little is known about the specific mechanisms, which caused treatment difficulties. This study focused on the molecular signatures underlying the pathological phenotypes of two diseases, with the hope of using statistical methods to identify the significant core genes. An unbiased Weighted Correlation Network Analysis (WGCNA) algorithm was used for endometrial transcriptome data analysis and the disease-related gene modules were screened out. Through enrichment analysis of the candidate genes, we found similarities between both diseases and shared enrichment of immune-related pathways. Therefore, we used immune algorithms to assess the infiltration of immune cells and found abnormal increases of CD8+T cells and neutrophils. In order to explore the molecular profile behind the immunophenotypic changes, we used the SVM algorithm and LASSO regression to identify the core genes with diagnostic capacity in both diseases and discussed their significance of immune disorders in the endometrium. In the end, the satisfactory diagnostic ability of these core genes was verified in the broader group. Our results demonstrated the presence of immune disorders in non-pregnancy tissues of RM and UI, and identified the core molecules of this phenotype, and discuss mechanisms. This provides exploratory evidence for the in-depth understanding of the mechanism of RM and UI and may provide potential targets for their future treatment.

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“…12 Jiang et al investigated the value of neutrophil and neutrophil to lymphocyte ratio (NLR) predicting RPL and proved the effectiveness of these two indicators. 13 Cimsir MT et al explored the value of fibrinogen to albumin ratio (FAR) in the prediction of RPL and found that FAR and NLR levels seem to be effective parameters for predicting RPL. 14…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐1β as clinically predictive risk marker for recurrent pregnancy loss in women positive for antinuclear antibody

Gao

Kong

Qing

et al. 2021

Int J Clinical Practice

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Aims Antinuclear antibody (ANA) was found to be associated with recurrent pregnancy loss (RPL). This study was designed to explore the immunological predictive indicators of RPL in women with positive ANA. Methods A retrospective case‐control study in a university hospital from March 2020 to January 2021 was performed, including 56 cases of women with RPL and 56 controls matched for age, all of which were positive for ANA. Levels of cytokines, lymphocyte subsets, immune globulin and complement in peripheral blood among two groups were compared. Statistical analyses in this study were performed using SPSS 25.0. Results The level of IL‐1β was higher in RPL women compared with controls according to univariable analysis and multivariable regression logistic analysis (7.67 [5.86‐11.35] vs 5.00 [5.00‐5.00], P = .000). After the cut‐off value of IL‐1β was set as 5.66 pg/mL, the prevalence of RPL was significantly elevated in the high IL‐1β group as compared with the low IL‐1β group (P < .05). The sensitivity and specificity of IL‐1β predicting RPL in ANA‐positive women were 76.8% and 91.1%, respectively. Conclusion Increased IL‐1β may play roles in the occurrence of RPL among women with positive ANA. The level of the IL‐1β has the potential to be a predictive indicator of RPL in women with positive ANA.

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Neutrophil and Neutrophil-to-Lymphocyte Ratio as Clinically Predictive Risk Markers for Recurrent Pregnancy Loss

Cited by 11 publications

References 27 publications

The peripheral and decidual immune cell profiles in women with recurrent pregnancy loss

The peripheral and decidual immune cell profiles in women with recurrent pregnancy loss

Investigation and Validation of Molecular Characteristics of Endometrium in Recurrent Miscarriage and Unexplained Infertility from a Transcriptomic Perspective

Interleukin‐1β as clinically predictive risk marker for recurrent pregnancy loss in women positive for antinuclear antibody

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