Neutrophil activation induced by the lectin KM+ involves binding to CXCR2

Yeast expressed ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM

“…It is noteworthy that n-ArtinM can form higher order oligomeric species (Fig. 1B; minor peaks at Svalues of approximately 6,7,9,10). A similar feature was observed in y-ArtinM (Fig.…”

“…n-ArtinM has been reported to induce haptotactic neutrophil migration by establishing concomitant interactions with CXCR2 on the cell surface and laminin in the extracellular matrix [6,29]. Therefore, we investigated the ability of y-ArtinM to induce haptotaxis.…”

“…ArtinM has a high specificity for Man␣1-3(Man␣1-6)Man, the trimannoside core of N-glycans [5]. By interacting with N-glycans of cell surface receptors, ArtinM triggers cellular responses such as neutrophil migration and activation by binding to the CXCR2 receptor [6,7], mast cell degranulation by binding to immunoglobulin (Ig) E or its receptor FcR [8,9], and interleukin12 (IL-12) production by inducing activation of macrophages and dendritic cells triggered by interaction with the Toll-like receptor 2 (TLR2) ectodomain [10,11].…”

Real-time monitoring and kinetic parameter estimation of the affinity interaction of jArtinM and rArtinM with peroxidase glycoprotein by the electrogravimetric technique

“…The protein CRDs (1 per protomer and 4 per whole protein) binds to D-mannose, preferentially recognizing N-linked glycans containing the trimannoside core Man␣1-3[Man␣1-6]Man (Jeyaprakash et al, 2004), such as those linked to the horseradish peroxidase (HRP), TLR2 (Coltri et al, 2008), laminin (Ganiko et al, 2005) and CXCR2 glycoproteins (Pereira- . Current investigations into the properties of ArtinM (Chahud et al, 2009;Coltri et al, 2008;Ganiko et al, 1998Ganiko et al, , 2005PanuntoCastelo et al, 2001;Pereira-Da-Silva et al, 2006;Rosa et al, 1999;Teixeira et al, 2006;Toledo et al, 2009) and, above all, its potential pharmaceutical applications, have been limited by the fact that this lectin corresponds to less than 0.5% of the total protein content of the extract of A. integrifolia seeds (Santos de Oliveira et al, 1994). Considering the interest in further exploring ArtinM properties, its cDNA was cloned and heterologously expressed in S. cerevisae and Escherichia coli (da Silva et al, 2005).…”

Nano drug delivery Study of Anticancer Properties on Jackfruit using QM/MM Methods

“…These nanotubes function with a larger inner volume to be used as the drug container, large aspect ratios for numerous functionalization attachments, and the ability to be readily taken up by the cell [46][47][48][49][50] .…”