Neutralizing Effects of Nectandra angustifolia Extracts against Bothrops neuwiedi Snake Venom

Abstract

Leaves extracts and essential oil of Nectandra angustifolia were explored for the first time for neutralization of Bothrops neuwiedi diporus snake venom. The ethanol extract was the most active and inhibited both venom activities (hemolytic and coagulant), while the oil was only active on the coagulant activity. These observations confirmed that certain medicinal plants from Corrientes and Chaco Provinces possess significant snake venom neutralizing capacity and need further examination for their active constituents. Analysis by GC and GC-MS of the essential oil and the enantiomeric excess found for -pinene, -pinene and limonene allowed a better characterization of this species.

Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms

“…There are few reports in the literature on the antiophidian properties of essential oils. The inhibitory effect of extracts and essential oils from Nectandra angustifolia leaves on the hemolysis and coagulation induced by B. neuwiedi venom were evaluated, showing that the essential oil was only effective in inhibiting coagulation [ 18 ]. The monoterpenes α-pinene, β-pinene and limonene were the major constituents identified in the essential oils evaluated and were considered by these authors as possible coagulation inhibitors.…”

“…Fibrinogen, a dimeric plasma glycoprotein of 340 kDa composed of six polypeptide chains (α, β, γ), participates in the coagulation cascade, and is converted by thrombin to fibrin monomers [ 37 ]. This protein has been extensively used for the evaluation of the potential fibrinogenolytic effects of snake venoms, and for the verification of potential antiophidian properties of natural compounds, mainly of plant origin [ 3 , 13 , 15 - 18 , 38 , 39 ].…”

“…Several studies are speculating on the use of plant extracts as sources of molecular prototypes which can be used in the treatment of snake envenomations, or to complement the traditional antivenoms, which have shown little effectiveness in minimizing the local damage [ 3 , 13 - 19 ]. However, studies employing essential oils for that purpose are still scarce [ 18 ].…”

Anti-5 ′ -Nucleotidases (5 ′ Aslam1, Fatima2, Khalid3 et al. 2021BioMed Research International2000View full textAdd to dashboardCite

“…Plants' secondary metabolites possess the ability to neutralize a range of enzymes present in snake venom such as 5 ′ -nucleotidases, protease, phospholipase A 2 , L-amino acid oxidase, and hyaluronidase. Various bioactive compounds have been reported in literature for their ability to inhibit one or more snake venom enzymes, for example, resveratrol [60,61], β-sitosterol [62][63][64], pentagalloyl glucopyranose [31,65], gallic acid [66,67], solanidane [68], alternamin [69], macrolobins A and B [70], and 8-methoxy coumestrol [71]. In this study, phytochemical screening of active fractions of plants' extracts showed the presence of various bioactive compounds such as flavonoids, phenols, saponins, tannins, terpenoids, steroids, and alkaloids.…”

Inhibition by essential oils of Mentha viridis and Mentha pulegium (Lamiaceae) in proteolysis, fibrinogenolysis and coagulation caused by venomous snakes

“…The fibrinogenolytic assay allows evaluating the action of proteases and their inhibitors on the proteolysis of fibrinogen molecules. Proteases present in the venoms from Bothrops genus generally induces a proteolytic action on α-chains of fibrinogen molecules, not being very effective in degrading β-chains and without action on γ-chain (Silva et al, 2012;Torres et al, 2012).…”