Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations

Robinson, Harriet L.; Montefiori, David C.; Johnson, R. Paul; Manson, Kelledy; Kalish, Marcia L.; Lifson, Jeff; Rizvi, Tahir A.; Lu, Shan; Hu, Shiu‐Lok; Mazzara, Gail P.; Panicali, Dennis; Herndon, James G.; Glickman, Rhona L.; Candido, Maria Angelito; Lydy, Shari L.; Wyand, Michael S.; McClure, Harold M.

doi:10.1038/8406

Cited by 353 publications

(199 citation statements)

References 42 publications

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“…In addition to malaria, similar observations have been made in several experimental models, thereby confirming the potential of the heterologous prime-boost immunization regimen to enhance the specific immune response against multiple pathogens (reviewed by Zavala et al 2001). It is also important to point out that this increase in the frequency of specific CD8 T cells described initially in mice was recently confirmed in several types of non-human primates (Kent et al 1998, Robinson et al 1999, Hanke et al 1999, Allen et al 2000.…”

Section: Malaria Liver Stagesmentioning

confidence: 55%

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are responsible for the infection of hundreds of millions of individuals every year. These parasites can deliver antigens to the host cell cytoplasm that are presented through MHC class I molecules to protective CD8 T cells. The in vivo priming conditions of specific CD8 T cells during natural infection are largely unknown and remain as an area that has been poorly explored. The antiparasitic mechanisms mediated by CD8 T cells include both interferon-γ -dependent and -independent pathways. The fact that CD8 T cells are potent inhibitors of parasitic development prompted many investigators to explore whether induction of these T cells can be a feasible strategy for the development of effective subunit vaccines against these parasitic diseases. Studies performed on experimental models supported the hypothesis that CD8 T cells induced by recombinant viral vectors or DNA vaccines could serve as the basis for human vaccination. Regimens of immunization consisting of two different vectors (heterologous prime-boost) are much more efficient in terms of expansion of protective CD8 T lymphocytes than immunization with a single vector. The results obtained using experimental models have led to clinical vaccination trials that are currently underway.

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Section: Malaria Liver Stagesmentioning

confidence: 55%

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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“…However, the relative importance of mobilizing each arm of the immune system and the means to induce each type of immunity are unclear. Although several successful passive immunization studies using neutralizing monoclonal antibodies in macaques strongly suggest that inducing broadly reactive neutralizing antibodies as part of any vaccine regimen would be beneficial [1][2][3][4][5], the levels and breadth of neutralizing antibodies induced by active vaccination have thus far proved insufficient to protect against infection [6][7][8]. It appears necessary that a vaccine must activate both cellular and humoral immune mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Live attenuated Listeria monocytogenes expressing HIV Gag: Immunogenicity in rhesus monkeys

Jiang

Rasmussen

Nolan

et al. 2007

Vaccine

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Induction of strong cellular immunity will be important for AIDS vaccine candidates. Natural infection with wild-type Listeria monocytogenes (Lm), an orally transmitted organism, is known to generate strong cellular immunity, thus raising the possibility that live attenuated Lm could serve as a vaccine vector. We sought to examine the potential of live attenuated Lm to induce cellular immune responses to HIV Gag. Rhesus macaques were immunized with Lmdd-gag that expresses HIV gag and lacks two genes in the D-alanine (D-ala) synthesis pathway. Without this key component of the bacterial cell wall, vaccine vector replication critically depends on exogenous D-ala. Lmdd-gag was given to animals either solely orally or by oral priming followed by intramuscular (i.m.) boosting; D-ala was co-administered with all vaccinations. Lmdd-gag and D-ala were well tolerated. Oral priming/oral boosting induced Gag-specific cellular immune responses, whereas oral priming/i.m. boosting induced systemic as well as mucosal anti-Gag antibodies. These results suggest that the route of vaccination may bias anti-Gag immune responses either towards T-helper type 1 (Th1) or Th2 responses; overall, our data show that live attenuated, recombinant Lmdd-gag was safe and immunogenic in primates.

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“…DNA vaccines are generally effective at stimulating CD8 responses, whilst subunit vaccines are more effective at eliciting antibody responses (Barouch et al, 2000;Earl et al, 2001Earl et al, , 2002Patterson et al, 2004;Robinson, 1999). Combined-modality DNA prime-protein boost vaccination strategies have been evaluated in the past (Cristillo et al, 2006;Letvin et al, 1997;Mooij et al, 2004;Otten et al, 2005;Pal et al, 2005Pal et al, , 2006Robinson et al, 1999). As with single-modality envelope protein-based vaccines (Berman et al, 1990;Bruck et al, 1994;Girard et al, 1991;Hu et al, 1992;Mooij et al, 1998;Verschoor et al, 1999), protection against homologous, non-pathogenic simian-human immunodeficiency virus (SHIV) can be obtained (Letvin et al, 1997;Pal et al, 2006;Robinson et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Immune-response profiles induced by human immunodeficiency virus type 1 vaccine DNA, protein or mixed-modality immunization: increased protection from pathogenic simian–human immunodeficiency virus viraemia with protein/DNA combination

Koopman

Mortier

Hofman

et al. 2008

Journal of General Virology

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Current data suggest that prophylactic human immunodeficiency virus type 1 (HIV) vaccines will be most efficacious if they elicit a combination of adaptive humoral and T-cell responses. Here, we explored the use of different vaccine strategies in heterologous prime–boost regimes and evaluated the breadth and nature of immune responses in rhesus monkeys induced by epidermally delivered plasmid DNA or recombinant HIV proteins formulated in the AS02A adjuvant system. These immunogens were administered alone or as either prime or boost in mixed-modality regimes. DNA immunization alone induced cell-mediated immune (CMI) responses, with a strong bias towards Th1-type cytokines, and no detectable antibodies to the vaccine antigens. Whenever adjuvanted protein was used as a vaccine, either alone or in a regime combined with DNA, high-titre antibody responses to all vaccine antigens were detected in addition to strong Th1- and Th2-type CMI responses. As the vaccine antigens included HIV-1 Env, Nef and Tat, as well as simian immunodeficiency virus (SIV)mac239 Nef, the animals were subsequently exposed to a heterologous, pathogenic simian–human immunodeficiency virus (SHIV)89.6p challenge. Protection against sustained high virus load was observed to some degree in all vaccinated groups. Suppression of virus replication to levels below detection was observed most frequently in the group immunized with protein followed by DNA immunization, and similarly in the group immunized with DNA alone. Interestingly, control of virus replication was associated with increased SIV Nef- and Gag-specific gamma interferon responses observed immediately following challenge.

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Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations

Cited by 353 publications

References 42 publications

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Live attenuated Listeria monocytogenes expressing HIV Gag: Immunogenicity in rhesus monkeys

Immune-response profiles induced by human immunodeficiency virus type 1 vaccine DNA, protein or mixed-modality immunization: increased protection from pathogenic simian–human immunodeficiency virus viraemia with protein/DNA combination

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