Neutralizing antibodies to bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) and its subtypes

Varela, Ana Paula Muterle; Holz, Carine Lidiane; Cibulski, Samuel Paulo; Teixeira, Thaís Fumaco; Antunes, Dinler Amaral; Franco, Ana Cláudia; Roehe, Luciana Ruschel; Oliveira, Martha Trindade; Campos, Fabrício Souza; Dezen, Diogenes; Cenci, Alexander; Brito, Wesley de Almeida; Roehe, Paulo Michel

doi:10.1016/j.vetmic.2009.10.016

Cited by 37 publications

(33 citation statements)

References 23 publications

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“…As previously reported, SN with a 24 hour incubation (Bitsch 1978, Perrin et al 1993) is expected to be more sensitive than the one hour incubation SN. In view of that, a similar study with a 24 hour incubation SN is presently being performed in order to examine the effect of a longer incubation on SN's sensitivity with different bovine herpesvirus types and subtypes (Varela et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Holz

Cibulski²,

Teixeira

et al. 2010

Pesq. Vet. Bras.

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The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted. RESUMO.-[Soroneutralização com diferentes tipos e subtipos de herpesvírus bovinos 1e 5.] O teste de soroneutralização (SN) é o método padrão para a mensuração de anticorpos neutralizantes para herpesvírus bovinos. Entretanto, com as subdivisões propostas destes agentes em tipos e subtipos, a definição de qual amostra utilizar como virus de desafio à SN pode ser difícil. Em vista disso, este estudo foi realizado para re-avaliar a sensibilidade de testes de SN utilizando diferentes tipos e subtipos de herpesvírus bovinos tipos 1 (BoHV-1) e 5 (BoHV-5) como amostras de desafio. Soros bovinos (n=810) foram coletados de duas regiões geográficas distintas e testados frente a amostras do tipo 1 (BoHV-1.1:Pesq. Vet. Bras. 30 (7):515-522, julho 2010Carine Lidiane Holz et al. 516 amostras "Los Angeles" e "EVI123/98", BoHV-1.2a: amostra "SV265/96") e três amostras do tipo 5 (BoHV-5a: "EVI88/95"; BoHV-5b: "A663" e BoHV-5c "ISO...

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Section: Discussionmentioning

confidence: 99%

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Holz

Cibulski²,

Teixeira

et al. 2010

Pesq. Vet. Bras.

View full text Add to dashboard Cite

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“…The sensitivity of the SN tests might have been increased by performing SNs against different challenge viruses. In a previous study, Varela et al (2010) detected about 30% more seropositive samples by testing sera against at least four different BoHV-1 and BoHV-5 strains. Nevertheless, the sensitivity of the SN as a diagnostic tool in the present study would remain significantly lower than that of the nPCR.…”

Section: Resultsmentioning

confidence: 95%

“…However, these calculations were based on serological assays that do not allow differentiation between BoHV-1 and BoHV-5 infections, in view of the wide cross-reactivity observed with antibodies of naturally infected animals (Varela et al 2010). Consequently, the actual prevalence of infection with each virus type remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Furtado¹,

Campos

Llambí³

et al. 2014

Arch. med. vet.

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“…Although most cases of neurological disease by bovine herpesviruses have been now attributed to BoHV-5, BoHV-1 has been occasionally associated with bovine meningoencephalitis (Furuoka et al 1995, Flores et al 1998, Varela et al 2010. Thus, we used a PCR strategy that would detect both BoHV-1 and BoHV-5 genomes while distinguishing between them based on the size of the amplicon.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

Pinto

Leite²,

Romijn³

et al. 2014

VR&R

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Bovine herpesvirus 5 (BoHV-5) is an important agent of meningoencephalitis in cattle and has been identified in outbreaks of neurological disease in several Brazilian states. However, no report of BoHV-5-associated neurological disease in Rio Janeiro state (RJ) has been published to date. This article reports a retrospective investigation for BoHV-5 performed in 146 brain specimens of cattle affected by neurological disease, submitted to the virology laboratory of the Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (PESAGRO-RJ) between 1998 and 2001. Most specimens came from herds in the RJ state (130 or 89%) and a few from Minas Gerais counties (16 or 11%), close to the RJ border. All brain samples examined in this study were previously diagnosed negative for rabies virus. The specimens were submitted to PCR/nested-PCR for BoHV-5 sequences and virus isolation. From 146 analysed brains samples 48 (33%) were positive in at least one of the tests (PCR or virus isolation), 20 samples (14%) were positive in both assays, 19 samples (13%) were positive in Nested-PCR (n-PCR) and nine samples (6%) were characterized as BoHV-5 after virus isolation. The northeast region of the RJ contributed with most clinical specimens (92/146 or 63%) and presented the highest positivity (28/92 or 30%). These results demonstrated BoHV-5 association with neurological disease in bovine herds of RJ and MG and should therefore be included in differential diagnosis of neurological disease in cattle. The limitations of each of the diagnostic assays used here in led us to recommend the concomitant use of PCR and virus isolation to improve the sensitivity of the diagnosis of BoHV-5 infection.

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Neutralizing antibodies to bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) and its subtypes

Cited by 37 publications

References 23 publications

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

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