Neutralizing antibodies to block viral entry and for identification of entry inhibitors

Tam, Ee Hong; Peng, Yu; Cheah, Megan Xin Yan; Yan, Chuan; Xiao, Tianshu

doi:10.1016/j.antiviral.2024.105834

Cited by 7 publications

(1 citation statement)

References 229 publications

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“…Therefore, antiviral drugs are designed to target viruses, especially virus-encoded enzymes, e.g., polymerases or proteases. As another option, drugs such as antibodies or aptamers have been developed. Although these compounds bind virions and prevent viruses from entering host cells, they cannot directly inactivate the virus.…”

Section: Introductionmentioning

confidence: 99%

Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer

Horníková,

Henke,

Kubát

et al. 2024

JACS Au

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Herein, we performed a simple virus capture and photoinactivation procedure using visible light on phosphatidylcholine vesicles. L-α-Phosphatidylcholine vesicles were enriched by viral receptors, GT1b gangliosides, and the nonpolar photosensitizer 5,10,15,20-tetraphenylporphyrin. These vesicles absorb in the blue region of visible light with a high quantum yield of antiviral singlet oxygen, O 2 ( 1 Δ g ). Through the successful incorporation of gangliosides into the structure of vesicles and the encapsulation of photosensitizers in their photoactive and monomeric state, the photogeneration of O 2 ( 1 Δ g ) was achieved with high efficiency on demand; this process was triggered by light, and specifically targeting/inactivating viruses were captured on ganglioside receptors due to the short lifetime (3.3 μs) and diffusion pathway (approximately 100 nm) of O 2 ( 1 Δ g ). Time-resolved and steady-state luminescence as well as absorption spectroscopy were used to monitor the photoactivity of the photosensitizer and the photogeneration of O 2 ( 1 Δ g ) on the surface of the vesicles. The capture of model mouse polyomavirus and its inactivation were achieved using immunofluorescence methods, and loss of infectivity toward mouse fibroblast 3T6 cells was detected.

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Section: Introductionmentioning

confidence: 99%

Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer

Horníková,

Henke,

Kubát

et al. 2024

JACS Au

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Exploring the ability of the MD+FoldX method to predict SARS-CoV-2 antibody escape mutations using large-scale data

Chi,

Barnes,

Patel

et al. 2024

Sci Rep

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The Pentamer glycoprotein complex inhibits viral Immediate Early transcription during Human Cytomegalovirus infections

Ohman,

Albright,

Gelbmann

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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The Pentamer complex of Human Cytomegalovirus (HCMV) consists of the viral glycoproteins gH, gL, UL128, UL130, and UL131 and is incorporated into infectious virions. HCMV strains propagated extensively in vitro in fibroblasts carry UL128, UL130, or UL131 alleles that do not make a functional complex and thus lack Pentamer function. Adding functional Pentamer to such strains decreases virus growth in fibroblasts. Here, we show that the Pentamer inhibits productive HCMV replication in fibroblasts by repressing viral Immediate Early (IE) transcription. We show that ectopic expression of the viral IE1 protein, a target of Pentamer-mediated transcriptional repression, complements the growth defect of a Pentamer-positive virus. Furthermore, we show that the Pentamer also represses viral IE transcription in cell types where HCMV in vitro latency is studied. Finally, we identify UL130 as a functional subunit of the Pentamer for IE transcriptional repression and demonstrate that cyclic AMP Response Element (CRE) and NFkB sites within the Major Immediate Early Promoter that drives IE1 transcription contribute to this repression. We conclude that the HCMV Pentamer represses viral IE transcription.

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Neutralizing antibodies to block viral entry and for identification of entry inhibitors

Cited by 7 publications

References 229 publications

Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer

Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer

Exploring the ability of the MD+FoldX method to predict SARS-CoV-2 antibody escape mutations using large-scale data

The Pentamer glycoprotein complex inhibits viral Immediate Early transcription during Human Cytomegalovirus infections

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