Neutralized electrolyzed water efficiently reduces Fusarium spp. in vitro and on wheat kernels but can trigger deoxynivalenol (DON) biosynthesis

Audenaert, Kris; Monbaliu, Sofie; Deschuyffeleer, Nick; Maene, Peter; Vekeman, Femke; Haesaert, Geert; Saeger, Sarah De; Eeckhout, Mia

doi:10.1016/j.foodcont.2011.08.024

Cited by 37 publications

(26 citation statements)

References 37 publications

(40 reference statements)

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“…Moreover, a recent study by Audenaert et al . () demonstrated the increased sensitivity of a tri 5 knockout mutant compared to its wild‐type parent strain, which indicated that biosynthesis of trichothecenes might also have a physiological meaning.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, intraculture differences have been observed by Ochiai et al (2007) who demonstrated differential tri5 transcript levels in fungal hyphae. Moreover, a recent study by Audenaert et al (2012) demonstrated the increased sensitivity of a tri5 knockout mutant compared to its wild-type parent strain, which indicated that biosynthesis of trichothecenes might also have a physiological meaning.…”

Section: Discussionmentioning

confidence: 99%

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Sublethal concentrations of azoles induce tri transcript levels and trichothecene production in Fusarium graminearum

Kulik

Łojko

Jestoi

et al. 2012

FEMS Microbiol Lett

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The effect of sublethal concentrations (below the recommended field doses) of propiconazole and tebuconazole on the amount of tri transcripts and accumulation of trichothecenes by three Fusarium graminearum isolates of 3ADON, 15ADON, and NIV chemotypes was examined on yeast extract sucrose agar (YES) medium. RT-qPCR analyses showed higher tri4, tri5, and tri11 transcript levels in cultures of all three F. graminearum isolates supplemented with sublethal concentrations of azoles as compared to those in nontreated control, although the fold changes in the amount of tri transcripts differed according to the type of azole used. Mycotoxin analysis revealed higher increase in trichothecene accumulation in most of the tebuconazole-treated samples of all chemotypes tested. A huge increase in all trichothecene compounds was revealed in samples of all F. graminearum isolates treated with 5 mg L(-1) of tebuconazole. An inducing effect of azoles on trichothecene accumulation in the grain was confirmed in an in planta experiment; however, the results obtained were inconsistent. A higher amount of trichothecenes and fungal DNA was quantitated in two grain samples treated with sublethal propiconazole concentrations. In contrast, no significant increase in trichothecene levels was revealed in grain samples treated with sublethal concentrations of tebuconazole.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sublethal concentrations of azoles induce tri transcript levels and trichothecene production in Fusarium graminearum

Kulik

Łojko

Jestoi

et al. 2012

FEMS Microbiol Lett

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“…Since NEW is non-toxic, non-corrosive and safe due to its physicochemical properties and a reverting capacity to ordinary water when diluted with tap water [17], NEW could be used for the application of safer, healthier and more acceptable methods for aflatoxin detoxification. Mounting evidence suggests that EOW has strong antifungal activity [18,19] and it is also effective in detoxifying AFB 1 in peanuts and maize [20,21]; however, there are some challenges faced in the development of this technology, such as the quick loss of activity in the presence of organic matter. Nevertheless, the application of NEW to detoxify aflatoxin-contaminated maize and the confirmation of the detoxification process in an in vivo experimental model have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Neutral Electrolyzed Water on Growth Performance, Biochemical Constituents, and Histopathological Changes in Turkey Poults during Aflatoxicosis

Gómez-Espinosa

Cervantes-Aguilar

Río-García

et al. 2017

Toxins

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Different in vitro and in silico approaches from our research group have demonstrated that neutral electrolyzed water (NEW) can be used to detoxify aflatoxins. The objective of this investigation was to evaluate the ability of NEW to detoxify B-aflatoxins (AFB1 and AFB2) in contaminated maize and to confirm detoxification in an in vivo experimental model. Batches of aflatoxin-contaminated maize were detoxified with NEW and mixed in commercial feed. A total of 240 6-day-old female large white Nicholas-700 turkey poults were randomly divided into four treatments of six replicates each (10 turkeys per replicate), which were fed ad libitum for two weeks with the following dietary treatments: (1) control feed containing aflatoxin-free maize (CONTROL); (2) feed containing the aflatoxin-contaminated maize (AF); (3) feed containing the aflatoxin-contaminated maize detoxified with NEW (AF + NEW); and (4) control feed containing aflatoxin-free maize treated with NEW (NEW). Compared to the control groups, turkey poults of the AF group significantly reduced body weight gain and increased feed conversion ratio and mortality rate; whereas turkey poults of the AF + NEW group did not present significant differences on productive parameters. In addition, alterations in serum biochemical constituents, enzyme activities, relative organ weight, gross morphological changes and histopathological studies were significantly mitigated by the aflatoxin-detoxification procedure. From these results, it is concluded that the treatment of aflatoxin-contaminated maize with NEW provided reasonable protection against the effects caused by aflatoxins in young turkey poults.

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“…Similar results have been described previously and F. poae is known to have a higher resistance level towards azole-type fungicides. 28,35 In this paper, we describe for the first time that fungicide application does not only cause a shift in the population towards an F. poae-dominated population, but also an accumulation of BEA is pointed out. To the best of our knowledge, this is the first study that delves into the impact of fungicides on cyclodepsipeptide concentrations.…”

Section: Discussionmentioning

confidence: 81%

“…25,26 Despite the fact that F. poae is a predominant species in Belgian wheat, 13,27 the concentrations of BEA were low, which suggests that this molecule is not necessary for F. poae to infect or colonize the wheat ear or that perhaps another species is responsible for BEA production in wheat. 13,28 With regard to ENNs, the highest correlation was observed with F. avenaceum. Fusarium avenaceum is a known potent producer of ENNs and is present in the Belgian FHB population.…”

Section: Discussionmentioning

confidence: 98%

Impact of fungicides and weather on cyclodepsipeptide‐producing Fusarium spp. and beauvericin and enniatin levels in wheat grains

et al. 2018

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Neutralized electrolyzed water efficiently reduces Fusarium spp. in vitro and on wheat kernels but can trigger deoxynivalenol (DON) biosynthesis

Cited by 37 publications

References 37 publications

Sublethal concentrations of azoles induce tri transcript levels and trichothecene production in Fusarium graminearum

Sublethal concentrations of azoles induce tri transcript levels and trichothecene production in Fusarium graminearum

Ameliorative Effects of Neutral Electrolyzed Water on Growth Performance, Biochemical Constituents, and Histopathological Changes in Turkey Poults during Aflatoxicosis

Impact of fungicides and weather on cyclodepsipeptide‐producing Fusarium spp. and beauvericin and enniatin levels in wheat grains

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