Neutralization of hemostatic disorders induced by Lachesis muta venom using Brazilian antivenoms

Muniz, Emiro G; Noronha, Maria das Dores N.; Saraiva, Maria das Graças Gomes; Monteiro, Wuelton Marcelo; Oliveira, Sâmella Silva de

doi:10.1016/j.toxicon.2020.12.013

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…There are some negative factors that resulting in deaths by accidents involving Lachesis snakes in Brazil: 1) limited availability of antivenoms, 2) difficulties in accessing health services in certain regions of the country, and 3) lack of a specific anti- Lachesis serum. Consequently, other types of antivenoms, e.g., anti- Bothrops serum and anti- Bothrops / Crotalus serum, have been inaccurately recommended to treat envenomations by L. muta in the absence of anti- Bothrops / Lachesis serum ( Magalhães et al, 2019 ; Muniz et al, 2021 ). These challenges involving the treatment of envenomations by Lachesis snakes strengthen the search for therapeutically useful adjuncts, with varespladib rising as a plausible tool.…”

Section: Discussionmentioning

confidence: 99%

Action of Varespladib (LY-315920), a Phospholipase A2 Inhibitor, on the Enzymatic, Coagulant and Haemorrhagic Activities of Lachesis muta rhombeata (South-American Bushmaster) Venom

et al. 2022

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Varespladib (VPL) was primarily developed to treat inflammatory disturbances associated with high levels of serum phospholipase A2 (PLA2). VPL has also demonstrated to be a potential antivenom support agent to prevent PLA2-dependent effects produced by snake venoms. In this study, we examined the action of VPL on the coagulant, haemorrhagic and enzymatic activities of Lachesis muta rhombeata (South-American bushmaster) venom. Conventional colorimetric enzymatic assays were performed for PLA2, caseinolytic and esterasic activities; in vitro coagulant activities for prothrombin time (PT) and activated partial thromboplastin time (aPTT) were performed in rat citrated plasma through a quick timer coagulometer, whereas the dimensions of haemorrhagic haloes obtained after i.d. injections of venom in Wistar rats were determined using ImageJ software. Venom (1 mg/ml) exhibited accentuated enzymatic activities for proteases and PLA2in vitro, with VPL abolishing the PLA2 activity from 0.01 mM; VPL did not affect caseinolytic and esterasic activities at any tested concentrations (0.001–1 mM). In rat citrated plasma in vitro, VPL (1 mM) alone efficiently prevented the venom (1 mg/ml)-induced procoagulant disorder associated to extrinsic (PT) pathway, whereas its association with a commercial antivenom successfully prevented changes in both intrinsic (aPTT) and extrinsic (PT) pathways; commercial antivenom by itself failed to avoid the procoagulant disorders by this venom. Venom (0.5 mg/kg)-induced hemorrhagic activity was slightly reduced by VPL (1 mM) alone or combined with antivenom (antivenom:venom ratio 1:3 ‘v/w’) in rats, with antivenom alone producing no protective action on this parameter. In conclusion, VPL does not inhibit other major enzymatic groups of L. m. rhombeata venom, with its high PLA2 antagonize activity efficaciously preventing the venom-induced coagulation disturbances.

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Section: Discussionmentioning

confidence: 99%

Action of Varespladib (LY-315920), a Phospholipase A2 Inhibitor, on the Enzymatic, Coagulant and Haemorrhagic Activities of Lachesis muta rhombeata (South-American Bushmaster) Venom

et al. 2022

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“…Its components (serine proteases and metalloproteases) can affect hemostatic mechanisms in different ways by interfering with both clotting factors and/or platelets, affecting their function; it is known that these proteases can also damage the integrity of blood vessel walls. 71 Another example is Sanguinaria canadensis, a well-known homeopathic medicine used for hot flashes. Sanguinarine is one of its six alkaloids and has been found to have a vasodilatory effect.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Menopause is more than Hot Flashes: What is Missing in Homeopathic Research? A Narrative Review

Macías-Cortés

2021

Homeopathy

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Background Menopausal complaints are frequently treated with homeopathy in daily practice worldwide. Recently, vasomotor symptoms have been understood to have implications as predictors of other important and long-term outcomes, causing increased risk of mortality and/or disability. Methods A comprehensive search of the literature was conducted to investigate whether homeopathic treatments for menopausal women with vasomotor symptoms have a positive effect on other important health outcomes associated with menopause, such as cardiovascular disease, neurocognitive impairment, metabolic and mood disorders, or osteoporosis. Results Though observational studies have shown encouraging results in reducing the severity and frequency of hot flashes in women treated with homeopathy, few randomized controlled trials have shown positive results. In most of the studies using homeopathy, the primary outcome is reduction in the frequency and severity of hot flashes, and other menopausal complaints are assessed secondarily as a part of the symptoms evaluated in the menopausal scales. Quality of life improves with homeopathic treatments for hot flashes, but there is scarce evidence of the effect of homeopathy on other health outcomes associated with menopause. Limited evidence exists in the case of menopausal women treated with individualized homeopathy for depression and metabolic disorders. Conclusion A more comprehensive approach for treating menopause in routine homeopathic practice constitutes a valuable opportunity to increase knowledge and high-quality research in this field. Future homeopathic research for menopause should be focused on well-designed, double-blind, placebo-controlled, randomized trials as well as on pragmatic trials to show whether homeopathic treatments for vasomotor symptoms can also improve outcomes that are well-known to increase the risk of mortality and/or disability.

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“…Para uma boa resposta ao tratamento, a soroterapia deve ser instituída o mais precocemente possível (Brasil, 2020). A eficácia do tratamento pode ser verificada a partir do exame do tempo de coagulação (TCoag) (Brasil, 2021;Muniz et al, 2021). Se, após 24 horas de aplicação de soroterapia, o sangue ainda continuar incoagulável por até 24 horas, uma nova dose capaz de neutralizar 150 mg de peçonha deve ser aplicada.…”

Section: Abordagem Terapêuticaunclassified

Acidentes laquéticos na Amazônia brasileira: aspectos clínicos e ecoepidemiológicos

Ferrareis,

Silva,

Will

et al. 2024

RSD

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Introduction: Ophidismsynonymous with snakebitescomprises envenomation cases resulting from snakebites. These cases are divided into four basic types in Brazil: bothropic, crotalic, elapidic, and laquetic. Objective: This article aims to describe and discuss the most relevant aspects of envenomation by Lachesis in the Brazilian Amazon. Methodology: To fulfill the objective, a literature review was conducted using the search strategy defined in the Lilacs, Scielo, and Pubmed databases. This search allowed the discussion of the following topics: (1) biology of snakes of the genus Lachesis, (2) ecology and epidemiology of laquetic accidents, (3) pathogenesis, (4) natural history and clinical presentation, (5) differential diagnosis, (6) laboratory evaluation, (7) therapeutic approach, and (8) prophylaxis and control. Results: Laquetic venom presents proteolytic, hemorrhagic, and coagulant activity, with reported neurotoxic action and related clinical alterations. Conducts should be based on information obtained through complete anamnesis, detailed clinical observation, and laboratory tests (especially coagulation time). Antilaquetic serum (ALS) or antibothropic-laquetic serum (ABLS) should be administered as early as possible to promote a positive impact on the victim's clinical evolution. Conclusion: Preventive measures should be adopted to minimize the risk of encounters between Homo sapiens and Lachesis and, consequently, the occurrence of envenomation.

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Neutralization of hemostatic disorders induced by Lachesis muta venom using Brazilian antivenoms

Cited by 7 publications

References 17 publications

Action of Varespladib (LY-315920), a Phospholipase A2 Inhibitor, on the Enzymatic, Coagulant and Haemorrhagic Activities of Lachesis muta rhombeata (South-American Bushmaster) Venom

Action of Varespladib (LY-315920), a Phospholipase A2 Inhibitor, on the Enzymatic, Coagulant and Haemorrhagic Activities of Lachesis muta rhombeata (South-American Bushmaster) Venom

Menopause is more than Hot Flashes: What is Missing in Homeopathic Research? A Narrative Review

Acidentes laquéticos na Amazônia brasileira: aspectos clínicos e ecoepidemiológicos

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