Neutralization map of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: domains recognized by monoclonal antibodies that prevent receptor recognition

Iorio, Ronald M.; Syddall, Richard J.; Sheehan, John P.; Bratt, Michael A.; Glickman, Rhona L.; Riel, Anne M.

doi:10.1128/jvi.65.9.4999-5006.1991

Cited by 108 publications

(57 citation statements)

References 42 publications

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“…4). NDV-HN antigenic site 23 is very close to antigenic sites 2 (␤5) and 3 (␤2L23, ␤3S4, and ␤3L23), which is in agreement with competition studies (25). Overlap with site 3 is probably an intramonomer overlap, and overlap with site 5 is probably an intermonomer overlap ( Fig.…”

Section: Locations Of Insertions and Deletions In Paramyxoviridae Hn supporting

confidence: 88%

Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin

Langedijk¹,

Daus²,

Oirschot³

1997

J Virol

142

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On the basis of the conservation of neuraminidase (N) active-site residues in influenza virus N and paramyxovirus hemagglutinin-neuraminidase (HN), it has been suggested that the three-dimensional (3D) structures of the globular heads of the two proteins are broadly similar. In this study, details of this structural similarity are worked out. Detailed multiple sequence alignment of paramyxovirus HN proteins and influenza virus N proteins was based on the schematic representation of the previously proposed structural similarity. This multiple sequence alignment of paramyxovirus HN proteins was used as an intermediate to align the morbillivirus hemagglutinin (H) proteins with neuraminidase. Hypothetical 3D structures were built for paramyxovirus HN and morbillivirus H, based on homology modelling. The locations of insertions and deletions, glycosylation sites, active-site residues, and disulfide bridges agree with the proposed 3D structure of HN and H of the Paramyxoviridae. Moreover, details of the modelled H protein predict previously undescribed enzymatic activity. This prediction was confirmed for rinderpest virus and peste des petits ruminants virus. The enzymatic activity was highly substrate specific, because sialic acid was released only from crude mucins isolated from bovine submaxillary glands. The enzymatic activity may indicate a general infection mechanism for respiratory viruses, and the active site may prove to be a new target for antiviral compounds.

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Section: Locations Of Insertions and Deletions In Paramyxoviridae Hn supporting

confidence: 88%

Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin

Langedijk¹,

Daus²,

Oirschot³

1997

J Virol

142

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“…The hemagglutinin-neuraminidase mediates attachment to cell receptor and possesses neuraminidase activity [Scheid and Choppin, 1973], the ability to cleave sialic acid, a component of the receptor. The neuraminidase active site residues localize to domains that are highly conserved among paramyxovirus HN proteins [Jorgensen et al, 1987;Iorio et al, 1989Iorio et al, , 1991. In HPIV2, these are the domains 168-RIPS-171, 228-NRKSCS-233, 393-GAEGR-397, and 502-RXNPT-506.…”

Section: Analysis Of the Putative Hemagglutinin Neuraminidase Amino Amentioning

confidence: 99%

Genetic diversity among human parainfluenza virus type 2 isolated in Croatia between 2011 and 2014

Šantak

Slović

Ljubin‐Sternak

et al. 2016

Journal of Medical Virology

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The dynamics and evolution of the human parainfluenza virus type 2 (HPIV2) in Croatia, and also globally, are largely unknown. Most HPIV2 infections are treated symptomatically outside the hospital setting. Thus, the diagnosis is missing making it difficult to follow the genetic variation and evolution of the HPIV2. This study explores hospitalized HPIV2 cases in Croatia during 4-year period (2011-2014). Most cases in this period were reported in October or November (68.75%) and most of patients were under 2 years of age (81.25%). For molecular analyses, we used the F and HN gene sequences and showed that although both regions are equally suitable for phylogenetic analyses it would be advantageous to use regions longer than 2 kb for HPIV2 analyses of isolates which are spatially and temporally closely related. We show here that the dominant cluster in this area was cluster G3 while only one strain isolated in this period was positioned in the distant cluster G1a. Further monitoring of the HPIV2 will determine whether cluster G3 will remain dominant or it will be overruled by cluster G1a. This will be important for the surveillance of virus circulation in population and significance of the viral infection. J. Med. Virol. 88:1733-1741, 2016. © 2016 Wiley Periodicals, Inc.

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“…Of the neutralizing epitopes identified in the HN protein in most NDV strains, 5,16,18,19 identical amino acid substitutions were found at positions 263, 494, and 514 in the virulent strains (ck/CH/LHLJ/1/06, go/CH/LHLJ/1/06, and go/ CH/HLJ/LL01/08) and avirulent strains (go/CH/LHLJ/2/06, go/CH/LFJ/1/06, and La Sota; Supplemental Table 4). Residue 347 of the HN protein is a major determinant of an immunodominant epitope, and the HN 347K mutation leads to antigenic variation in genotype VII NDV strains.…”

Section: Genomic Characterization and Phylogenetic Analysismentioning

confidence: 99%

Genetic, antigenic, and pathogenic characteristics of Newcastle disease viruses isolated from geese in China

Sun

Gao

et al. 2017

J VET Diagn Invest

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Four Newcastle disease virus (NDV) strains were isolated from domestic, commercial geese that showed clinical signs that were believed to be the result of NDV infections. The genetic, antigenic, and pathogenic characteristics of the 4 NDVs were compared with those of NDV strains that were isolated from chickens. The complete genomes of 2 of the NDV strains contained 15,186 nucleotides (nt); the other 2 contained 15,192 nt, and exhibited the typical genomic organization of genotype II NDV and molecular characteristics of VIId NDVs. Phylogenetic analysis confirmed that the genotype II and VIId NDVs that were isolated from geese belonged to the same clusters as the corresponding genotypes of the chicken isolates. A serologic assay demonstrated that the antigenic relatedness among the NDVs was associated with their genotypes, rather than their hosts, and that amino acid substitutions in the F and/or HN proteins may contribute to the antigenic differences among these NDV genotypes. Geese infected with genotype VIId NDVs that were isolated from geese and chickens showed similar pathologic characteristics. NDVs that were isolated from geese did not differ in genetic, serologic, and pathogenic characteristics from those isolated from chickens, indicating that these NDVs were derived from chicken NDVs. Given the significance of geese in NDV epidemiology, effective biosecurity measures should be adopted to prevent the interspecies transmission of NDVs.

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Neutralization map of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: domains recognized by monoclonal antibodies that prevent receptor recognition

Cited by 108 publications

References 42 publications

Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin

Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin

Genetic diversity among human parainfluenza virus type 2 isolated in Croatia between 2011 and 2014

Genetic, antigenic, and pathogenic characteristics of Newcastle disease viruses isolated from geese in China

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