Neutral Liposomes and DNA Transfection

Pisani, Michela; Mobbili, Giovanna; Bruni, P.

doi:10.5772/21283

Cited by 9 publications

(13 citation statements)

References 105 publications

(85 reference statements)

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“…Катионные липосомы могут сформироваться только из одного КЛ, однако добавление в их состав дополнительного нейтрального липида-хелпера может увеличить ЭТ [56]. Среди липидов-хелперов наиболее часто используются 1,2-диолеоил-sn-глицеро-3-фосфоэтаноламин (13a, DOPE) [19] и фосфатидилхолин (14, PC) [57].…”

Section: липиды-хелперыunclassified

“…Фосфатидилхолин (14, PC) представляет собой группу фосфолипидов, содержащих остаток холина в качестве гидрофильной группы и фосфатидную кислоту с различными ацильными остатками в качестве гидрофобного домена [57]. Однако использование PC в качестве липида-хелпера, формирующего ламеллярную структуру в составе катионных липосом, приводило к понижению ЭТ, в отличие от DOPE [56].…”

Section: липиды-хелперыunclassified

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Сationic liposomes as delivery systems for nucleic acids

et al. 2020

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Objectives. Gene therapy is based on the introduction of genetic material into cells, tissues, or organs for the treatment of hereditary or acquired diseases. A key factor in the success of gene therapy is the development of delivery systems that can efficiently transfer genetic material to the place of their therapeutic action without causing any associated side effects. Over the past 10 years, significant effort has been directed toward creating more efficient and biocompatible vectors capable of transferring nucleic acids (NAs) into cells without inducing an immune response. Cationic liposomes are among the most versatile tools for delivering NAs into cells; however, the use of liposomes for gene therapy is limited by their low specificity. This is due to the presence of various biological barriers to the complex of liposomes with NA, including instability in biological fluids, interaction with serum proteins, plasma and nuclear membranes, and endosomal degradation. This review summarizes the results of research in recent years on the development of cationic liposomes that are effective in vitro and in vivo. Particular attention is paid to the individual structural elements of cationic liposomes that determine the transfection efficiency and cytotoxicity. The purpose of this review was to provide a theoretical justification of the most promising choice of cationic liposomes for the delivery of NAs into eukaryotic cells and study the effect of the composition of cationic lipids (CLs) on the transfection efficiency in vitro.Results. As a result of the analysis of the related literature, it can be argued that one of the most promising delivery systems of NAs is CL based on cholesterol and spermine with the addition of a helper lipid DOPE. In addition, it was found that varying the composition of cationic liposomes, the ratio of CL to NA, or the size and zeta potential of liposomes has a significant effect on the transfection efficiency.Conclusions. Further studies in this direction should include optimization of the conditions for obtaining cationic liposomes, taking into account the physicochemical properties and established laws. It is necessary to identify mechanisms that increase the efficiency of NA delivery in vitro by searching for optimal structures of cationic liposomes, determining the ratio of lipoplex components, and studying the delivery efficiency and properties of multicomponent liposomes.

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Section: липиды-хелперыunclassified

Сationic liposomes as delivery systems for nucleic acids

et al. 2020

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“…The drawback, however, is that a nanoparticle with a surface too rich in positive charges can disrupt the membrane [42]. The presence of DOPE in this cationic liposomes could have the dual function of reducing their toxicity [28] and potentially eliciting an inverted hexagonal phase transition that facilitates the delivery to the cytoplasm [43]. Aside from their cationic nature, there have been reports of liposomal toxicities that may be important in the context of their efficacy.…”

Section: Nanoparticle Toxicity and Azd6244 Deliverymentioning

confidence: 99%

Efficacy of DOPE/DC-Cholesterol Liposomes and GCPQ Micelles As AZD6244 Nanocarriers in a 3D Colorectal Cancer In Vitro Model

López-Dávila

Magdeldin

Welch

et al. 2016

Nanomedicine (Lond.)

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“…The neutral lipid alters the physical property of the delivery vehicle and also reduces the toxicity of the system, in a way that favours some of the most complex steps of the whole mechanism of transfection: the consequence is that both actions affect the quality of the transfection. [18] Each lipid has different structural properties, such as head group size and hydrocarbon tail length. These properties confer distinct characteristics to the lipid/DNA complex, which in turn affect association with and uptake into the cell.…”

Section: Lipids and Their Role In The Formulation Of Liposomesmentioning

confidence: 99%

“…Studies have shown that higher efficiencies could be obtained by adding a neutral lipid to the lipoplexes with the role of transfection helper. [18] Cholesterol Cholesterol is a neutral lipid which most importantly acts as a stabilizing agent.…”

Section: Lipids and Their Role In The Formulation Of Liposomesmentioning

confidence: 99%

In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease

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Neutral Liposomes and DNA Transfection

Cited by 9 publications

References 105 publications

Сationic liposomes as delivery systems for nucleic acids

Сationic liposomes as delivery systems for nucleic acids

Efficacy of DOPE/DC-Cholesterol Liposomes and GCPQ Micelles As AZD6244 Nanocarriers in a 3D Colorectal Cancer In Vitro Model

In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease

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