Neutral lipids of fungus Zoophthora

Abstract

Cultivation of Zoophthora (Erynia) neoaphidis, in comparison with other species of Zoophthora genus, requires more complex nitrogen source. The best mycelium growth was obtained when 8 day old mycelium was used as an inoculum for liquid culture. On the basis of Gas Chromatographyblass Spectrometry (GCIMS) methods, the structures of the neutral lipids produced by this fungus species, were determined. Decanoic acid was the most characteristic component (over 45%). The level of hexadecanoic and octadecanoic acid was relatively lower, although their contribution as "bonded" acid in diacylglyceride (DG) and triacylglyceride (TG) fractions, was significant. Contrary to Zoophthora (Erynia) ovispora, the presence of campesterol andlor dihydrobrassicosterol was proved.