Neutral lipid alterations in Human Herpesvirus 8-infected HUVEC cells and their possible involvement in neo-angiogenesis

Angius, Fabrizio; Uda, Susumu; Piras, Enrica; Spolitu, Stefano; Ingianni, Angela; Batetta, Barbara; Pompei, Raffaello

doi:10.1186/s12866-015-0415-7

Cited by 25 publications

(43 citation statements)

References 35 publications

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“…A possible association of HHV8 infection with type-2 diabetes has recently been proposed [16][17][18][19]. Insulin hyper-secretion and consumption is considered a possible initiating event of obesity and diabetes, and discovering the causes that induce insulin hypersecretion could help to find a solution for diabetes treatment [20].…”

Section: Resultsmentioning

confidence: 99%

“…HHV8-positive BC3 cells were grown in RPMI-1640 medium with 10% fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA) as previously described [16,17]. A pool stock of HUVEC cells (Thermo Fisher Scientific, Waltham, MA, USA) was grown in a M200 medium (Thermo Fisher Scientific, Waltham, MA, USA) with low serum growth supplement (LSGS, Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Cell infection was assessed by two molecular biology assays as previously reported [16]. In brief, RT-PCR was performed in order to detect the transcripts of the orf26, orf50 and orf73 genes, and the viral protein expression of K8.1 (mouse monoclonal) and LANA (rat monoclonal) were assessed by western blot in HHV8-infected HUVEC cells.…”

Section: Characterization Of Hhv8-infection In Infected Huvec Cellsmentioning

confidence: 99%

“…The protein content of each sample was determined by the BCA assay (SigmaAldrich, Milan, Italy), and processed as previously described [16]. In brief, protein samples (12 μg/lane) were separated by electrophoresis (acrylamide precast gel; Bio-Rad, Laboratories Inc., Milan, Italy) and transferred to a 0.45 μm pore size nitrocellulose (Millipore, Milan, Italy) by standard electro-blotting procedure.…”

Section: Western Blot For Ppi3k Detection In Huvec Cellsmentioning

confidence: 99%

“…HHV8-infection has been reported to induce profound changes in the behaviour of human umbilical vein endothelial cells (HUVEC) [8,[11][12][13], by triggering an over-expression of the insulin receptor, a higher glucose consumption, and production of hypoxia-induced factors [11][12][13][14][15]. Cellular insulinuptake was detected in the viral lytic phase with a peak during the late-latent phase, whereas glucoseconsumption was decreased in the lytic infection, and remarkably increased in the latent phase [16,17]. The increased insulin-uptake induced an increase of glucose accumulation in HUVEC cells.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Human Herpesvirus 8 antibodies affect both insulin and glucose uptake by virus-infected human endothelial cells

Angius

Piras

Spolitu

et al. 2018

J Infect Dev Ctries

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Introduction: Human Herpesvirus 8 (HHV8) is known to be the cause of the malignant tumour named Kaposi's sarcoma. It is believed to induce an intense modification of cell metabolism in endothelial cells. In this work we analysed the role of anti-HHV8 antibodies in both the insulin and glucose uptake of HHV8-infected primary human endothelial cells (HUVEC). Methodology: Western blotting, immunofluorescence and radiolabelled glucose were employed to assess the pPI3K expression, insulin binding and glucose-uptake by HUVEC cells, respectively. Results: We confirmed that HHV8-infection is able to enhance both insulin binding and glucose-uptake in HHV8-infected primary endothelial cells; in addition, we found that anti-HHV8 specific antibodies are able to further increase both insulin and glucose uptake during the late latent phase of HHV8-infection in vitro. Conclusions: These findings suggest that a specific immune response to HHV8-infection may cooperate in boosting the cell metabolism, further enhancing the already increased insulin binding and glucose-uptake in HHV8-infected cells, which is a peculiar property of several oncogenic viruses.

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Section: Resultsmentioning

confidence: 99%

Section: Cells and Virusesmentioning

confidence: 99%

Section: Characterization Of Hhv8-infection In Infected Huvec Cellsmentioning

confidence: 99%

Section: Western Blot For Ppi3k Detection In Huvec Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Anti-Human Herpesvirus 8 antibodies affect both insulin and glucose uptake by virus-infected human endothelial cells

Angius

Piras

Spolitu

et al. 2018

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

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The interplay between lipid droplets and virus infection

Wang

Xiao

et al. 2023

Journal of Medical Virology

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As an intracellular parasite, the virus usurps cellular machinery and modulates cellular metabolism pathways to replicate itself in cells. Lipid droplets (LDs) are universally conserved energy storage organelles that not only play vital roles in maintaining lipid homeostasis but are also involved in viral replication. Increasing evidence has demonstrated that viruses take advantage of cellular lipid metabolism by targeting the biogenesis, hydrolysis, and lipophagy of LD during viral infection. In this review, we summarize the current knowledge about the modulation of cellular LD by different viruses, with a special emphasis on the Hepatitis C virus, Dengue virus, and SARS-CoV-2.

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The Role of Human Herpesvirus 8 in Diabetes Mellitus Type 2: State of the Art and a Medical Hypothesis

Pompei

2015

Advances in Experimental Medicine and Biology

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Diabetes is a common chronic disease due to an altered glucose metabolism, caused by the quantitative and/or qualitative dysfunction of the insulin hormone. Two types of diabetes are recognized: juvenile diabetes, or type 1, which has an autoimmune origin, and adult diabetes, or type 2 (DMT2), which covers 90-95 % of all diabetic patients.The causes of DMT2 are not yet clear: heredity, life style, nutrition, and environment are considered the main risk factors. Several viral infections, namely cytomegalovirus, coxsackie and other enteroviruses, rubella and hepatitis C virus, have been claimed to be associated with some forms of diabetes. The direct role of viruses as a cause or as a risk of type 1 diabetes has been amply described in several recent reviews. Therefore, this review focuses attention on the role of a human herpes pathogenic virus in the onset of DMT2. By carrying out an analysis of recent literature, we describe the findings reported on an extremely deceitful virus, such as Human Herpes virus 8, and present a medical hypothesis on a possible relationship between this virus and DMT2.

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Neutral lipid alterations in Human Herpesvirus 8-infected HUVEC cells and their possible involvement in neo-angiogenesis

Cited by 25 publications

References 35 publications

Anti-Human Herpesvirus 8 antibodies affect both insulin and glucose uptake by virus-infected human endothelial cells

Anti-Human Herpesvirus 8 antibodies affect both insulin and glucose uptake by virus-infected human endothelial cells

The interplay between lipid droplets and virus infection

The Role of Human Herpesvirus 8 in Diabetes Mellitus Type 2: State of the Art and a Medical Hypothesis

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