Neutral and basic amino acid concentrations in plasma during the day in subjects fed with two model rural and two model urban Mexican diets

Abstract

The purpose of the study was to measure the variation in concentrations of plasma neutral and basic amino acids during the day in subjects fed two Mexican model rural diets, one containing 55% (R55) and the other 70% (R70) of energy as carbohydrates, and two model urban diets with the same two proportions of carbohydrates (U55 and U70). The R55 and R70 diets contained 1.35- and 1.69-fold more fiber than the U55 and U70 diets, respectively. Eight female volunteers were adapted to each of the four diets for 3 d before the day of blood sampling. Protein and energy intakes were adjusted to each subject for a consumption of 1 g protein/kg body wt and 150.7 kJ/kg body wt. Blood samples were withdrawn at 0700, 0930, 1100, 1500, 1900, 2300, and 0300. Only plasma concentrations of alanine changed during the day, dropping significantly (P<0.05) at 2300 and 0300 with the U70 diet. Urban diets produced significantly higher plasma isoleucine and valine values than did the rural diets at some sampling times. Plasma phenylalanine was significantly higher with the U70 diet at 2300 than with the other three diets. Alanine plasma concentrations were significantly higher with the U55 diet at 1900 and significantly lower with the R55 diet at 0930 with respect to the other diets. Lysine was significantly higher at 0700 with the U70 diet than with the other three diets. No other significant changes were observed. These results show the stability of the plasma amino acid profile despite the consumption of different diets in physiologic proportions. Possibly, some of the changes observed in the plasma amino acids can be explained by the high proportion of dietary fiber in the Mexican rural diets.

Relationship between Branched-Chain Amino Acids, Metabolic Syndrome, and Cardiovascular Risk Profile in a Chinese Population: A Cross-Sectional Study

“…However, the idea that BCAAs or their supplementation might have a positive role in preventing metabolic disease is controversial. It is thought that excess amino acids intake and impairments in BCAAs metabolism could result in accumulation of serum BCAAs in animal and clinical investigations [ 22 , 23 ]. Therefore, BCAAs are usually acknowledged evidence of metabolism dysfunction.…”

Omental adipose tissue gene expression, gene variants, branched-chain amino acids, and their relationship with metabolic syndrome and insulin resistance in humans

“…Our results revealed some of the factors involved in the development of the AT dysfunction associated with obesity. The overnutrition that is reflected by an increase in plasma amino acid concentrations (Tovar et al 1996) produces an excess of amino acids that must be rapidly oxidized in the liver by the amino acid-catabolizing enzymes. However, BCAAs undergo a different metabolic fate due to the absence of BCAT2 in the liver.…”

Plasma branched-chain and aromatic amino acid concentration after ingestion of an urban or rural diet in rural Mexican women

“…Plasma glucose, total cholesterol, triglycerides and HDL-cholesterol were measured enzymatically on an automated Synchron CX autoanalyzer (Beckman, CA, USA). Amino acid analysis was performed as previously described [ 8 ]. Briefly, 50 mg sulfosalicylic acid was added to 1 mL plasma to deproteinize the sample.…”

“…Conversely, the urban middle-class diet includes more animal foods and refined products. Interestingly, ingestion of these two diets induces different changes in plasma amino acid concentrations throughout the day [ 8 ]. Furthermore, people living in rural areas are prone to move to urban cities looking for better opportunities and this is associated with a dramatic change in the feeding behavior that includes changes in the type of protein consumed [ 9 ].…”