Neutral Amino Acid Transport in Sheep External Intercostal Muscle

Forsberg, Neil E.; Kaneps, Andris J.; Riebold, Thomas W.

doi:10.4141/cjas88-046

Cited by 4 publications

(1 citation statement)

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“…LH concentration in sheep’s blood is one of the most common and measurable parameters prior to estrus and ovulation. − At lower concentrations of LH in ewe, the chances of success for artificial insemination decreases drastically. As such, the detection and measurement of concentrations of LH in sheep is important from an economic (and farming community) point of view . Current method of analysis of LH involves immunoassay using antibodies specific toward LH .…”

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confidence: 99%

Design and Development of a Field Applicable Gold Nanosensor for the Detection of Luteinizing Hormone

Zambre¹,

Chanda²,

Prayaga³

et al. 2012

Anal. Chem.

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In this paper, we describe a novel strategy for the fabrication of a nanosensor for detecting luteinizing hormone (LH) of sheep using a gold nanoparticle-peptide conjugate. A new peptide sequence "CDHPPLPDILFL" (leutinizing hormone peptide, LHP) has been identified, using BLAST and Clustal W analysis, to detect antibody of LH (sheep). LHP has been synthesized and characterized, and their affinity toward anti-LH was established using enzyme linked immunosorbant assay (ELISA) technique. The thiol group in LHP directly binds with gold nanoparticles (AuNPs) to yield AuNP-LHP construct. Detailed physicochemical analysis of AuNP-LHP construct was determined using various analytical techniques. Nanosensor using gold nanoparticle peptide conjugate was developed on the basis of competitive binding of AuNP-LHP and LH toward anti-LH. Nitrocellulose membrane, precoated with anti-LH, was soaked in the mixture of AuNP-LHP and sample of analysis (LH). In the absence of LH (sheep), anti-LH coated on the membrane binds with AuNP-LHP, leading to a distinctive red color, while in the presence of LH, no color appeared in the membrane due to the interaction of anti-LH with LH thereby preventing the binding of AuNP-LHP with membrane bound anti-LH. The sensor assay developed in this study can detect LH (sheep) up to a minimal concentration of ∼50 ppm with a high degree of reproducibility and selectivity. The gold-nanoparticle-peptide based nanosensor would be a simple, portable, effective, and low cost technique for infield applications.

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confidence: 99%