Neurovascular Transfer Does Not Cause Skeletal Muscle Fiber Degeneration

Demeester, J.; Hasan, Jafar; Urbanchek, Melanie G.; Kuzon, William M.

doi:10.1097/01.prs.0000206356.77759.1a

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Skeletal muscle injury is one of the most common injuries in forensic science [15]. After acute skeletal muscle injury, the injury site experiences damage, degeneration, and necrosis of the muscle fiber structure, after which inflammatory cells and factors infiltrate the injury site [16][17][18][19][20]. Neutrophils play important roles in the early stages of inflammation after various tissue injuries.…”

Section: Discussionmentioning

confidence: 99%

Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

Niu

et al. 2020

Forensic Sciences Research

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2020): Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats, Forensic Sciences Research, ABSTRACT In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n ¼ 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm 2 (0.5 mm Â 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 mm 2 to 15 mm 2 , the FPR was 4.28%-12.14%, the FNR was 12.42%-64.08%, the sensitivity was 35.92%-87.58%, the specificity was 87.86%-95.72%, the Youden index was 0.316-0.754, accuracy was 82.80%-88.30%, and the AUC was 0.771-0.826. The AUC was largest when the cut-off value of the staining area was 12 mm 2 . Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

Niu

et al. 2020

Forensic Sciences Research

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“…8,9 In adult skeletal muscles, terminally differ-entiated satellite cells can fuse to their associated myofibers, express embryonic myosin heavy chain (eMyHC; a special type of myosin heavy chain that is expressed exclusively during embryonic development and muscle regeneration), and function in muscle contraction. 10,11 For this reason, eMyHCcontaining fibers are regarded as a marker of successful regeneration induced by satellite cell differentiation. 10 Satellite cells are activated in response to specific stimuli, such as local muscle damage.…”

mentioning

confidence: 99%

“…MyoD expression is necessary for initiation of myogenesis and thus indicates irreversible differentiation of satellite cells . In adult skeletal muscles, terminally differentiated satellite cells can fuse to their associated myofibers, express embryonic myosin heavy chain (eMyHC; a special type of myosin heavy chain that is expressed exclusively during embryonic development and muscle regeneration), and function in muscle contraction . For this reason, eMyHC‐containing fibers are regarded as a marker of successful regeneration induced by satellite cell differentiation …”

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confidence: 99%

Electrical stimulation influences satellite cell differentiation after sciatic nerve crush injury in rats

et al. 2015

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Neurovascular Transfer Does Not Cause Skeletal Muscle Fiber Degeneration

Abstract: After neurovascular muscle transfer (with ischemia times up to 3 hours), ischemia-induced muscle fiber degeneration and regeneration does not occur. Muscle fiber degeneration is not responsible for the force deficits observed after microneurovascular skeletal muscle transfer.

Cited by 2 publications

References 41 publications

Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

Electrical stimulation influences satellite cell differentiation after sciatic nerve crush injury in rats

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