Neurotropism and behavioral changes associated with Zika infection in the vector
 Aedes aegypti

Gaburro, Julie; Bhatti, Asim; Harper, Jenni; Jeanne, Isabelle; Dearnley, Megan; Green, Diane; Nahavandi, Saeid; Paradkar, Prasad N.; Duchemin, Jean‐Bernard

doi:10.1038/s41426-018-0069-2

Cited by 29 publications

(28 citation statements)

References 62 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fecundity analysis, there were no differences between ZIKV infected and uninfected mosquitoes, even when using another laboratorial approach. 21 In contrast, a previous study showed that DENV infected Ae. aegypti females exhibited lower fecundity (egg quantity).…”

Section: Discussionmentioning

confidence: 84%

Zika infection decreases Aedes aegypti locomotor activity but does not influence egg production or viability

Padilha

Resck

Cunha

et al. 2018

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

BACKGROUND Zika has emerged as a new public health threat after the explosive epidemic in Brazil in 2015. It is an arbovirus transmitted mainly by Aedes aegypti mosquitoes. The knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.OBJECTIVES We aimed to investigate the effects of Zika virus (ZIKV) infection in the behaviour of Ae. aegypti females by analysing the locomotor activity, egg production and viability.METHODOLOGY Ae. aegypti females were orally infected with ZIKV through an artificial feeder to access egg production, egg viability and locomotor activity. For egg production and viability assays, females were kept in cages containing an artificial site for oviposition and eggs were counted. Locomotor activity assays were performed in activity monitors and an average of 5th, 6th and 7th days after infective feeding was calculated.FINDINGS No significant difference in the number of eggs laid per females neither in their viability were found between ZIKV infected and non-infected females, regardless the tested pair of mosquito population and virus strain and the gonotrophic cycles. Locomotor activity assays were performed regardless of the locomotor activity in ZIKV infected females was observed, in both LD and DD conditions.MAIN CONCLUSIONS The lower locomotor activity may reduce the mobility of the mosquitoes and may explain case clustering within households reported during Zika outbreaks such as in Rio de Janeiro 2015. Nevertheless, the mosquitoes infected with ZIKV are still able to disseminate and to transmit the disease, especially in places where there are many oviposition sites.

show abstract

Section: Discussionmentioning

confidence: 84%

Zika infection decreases Aedes aegypti locomotor activity but does not influence egg production or viability

Padilha

Resck

Cunha

et al. 2018

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Interestingly, these authors also stated that heads and salivary glands were the only tissues where viral antigens continued to accumulate until 21 dpi, while other infected tissues showed a decrease in the infection rate. Finally, a recent study showed that the same DENV2 strain is able to replicate in dissected Aedes aegypti (Mexico origin) primary neuron culture, with the presence of viral antigens 38 . Oviposition behaviour, as well as the related odour choice modification by infection, occurred between 3 to 5 days post BM 46 , with increase of virus load and prevalence in female heads.…”

Section: Discussionmentioning

confidence: 99%

“…Aedes mosquitoes after artificial intrathoracic infection showed an increased total level of activity 2 to 6 dpi, with similar daily activity patterns between DENV2 infected and uninfected mosquitoes 37 . Another study showed that infected Aedes aegypti females infected with DENV2 had an increased activity and space occupation in laboratory conditions 38 . Behavioural changes post infection underlie a potential way for the virus to manipulate the vector.…”

Section: Discussionmentioning

confidence: 99%

“…Aedes aegypti is the main vector for dengue virus and is a major global public health problem 31 . Some studies have reported behavioural changes due to arbovirus infection in mosquito vectors, such as during blood-feeding 32–35 , host-seeking 36 and locomotion 37,38 . However, no study has reported a change in oviposition behaviour of the vector due to viral infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dengue virus infection changes Aedes aegypti oviposition olfactory preferences

Gaburro

Paradkar

Klein

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

14Aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory 15 preferences and are capable of olfactory learning especially when seeking their required 16 environmental conditions to lay their eggs. In this study, we showed that semiochemical 17 conditions during Aedes aegypti larval rearing affected future female choice for oviposition: 18 water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole 19 reared mosquitoes preferred skatole sites. Using two independent behavioural assays, we 20 showed that this skatole preference was lost in mosquitoes infected with dengue virus. Viral 21 RNA was extracted from infected female mosquito heads, and an increase of virus load was 22 detected from 3 to 10 days post infection, indicating replication in the insect head and 23 possibly in the central nervous system. Expression of selected genes, potentially implied in 24 olfactory learning processes, were also altered during dengue infection. Based on these 25 results, we hypothesise that dengue virus infection alters gene expression in the mosquito's 26 head and is associated with a loss of olfactory preferences, possibly modifying oviposition 27 site choice of female mosquitoes.It is important for insects to identify and localize sites for food resources, mating and egg 30 laying to successfully survive and reproduce. It is generally known that insects, including 31 mosquitoes, are capable of information retention, acquired either during larval development, 32 hatching or foraging, and that new information could influence their behaviour 1 . In this 33 study, we define the term of "olfactory learning" as change of olfactory preferences induced 34 by exposure to odour. 35In insect vectors, such as mosquitoes, learning from its own experience could influence the 36 vector behaviour, such as the potential preferences in vector-host interactions 2,3 , which can 37 be of great interest to epidemiologists. Mosquitoes are also capable of associative learning, 38 which is the process of learning the association between two stimuli 4 , in the context of 39 feeding 5 , or responding to odour cues 6-10 . Also, learning in mosquito vectors could 40 significantly influence virus transmission through host contact and survival rate 9 . In nature, 41 environments are constantly subjected to changes, especially chemical cues involved in 42 potential food or oviposition site 11 . In order to respond to such rapid change and improve its 43 searching efficiency, a vector needs to adapt by learning to respond to odour cues 6 . The 44 mosquito experience to olfactory cues from larval, pupal or early adult vector environment 45 could influence its behaviour. 46After a blood meal from a host, female mosquitoes look for suitable oviposition sites to 47 guarantee success of the offspring 12 . Among different factors, chemical cues play a key role 48 in determining location for mosquito to lay its eggs 13,14 . The chemical signature of an ideal 49 oviposition site is not certai...

show abstract

“…Changes with DENV clearly affect the physiology and impact behavior in A. aegypti due in part to the infection of olfactory organs that changes expression levels of OBP10 and OBP22 (odorant-binding proteins) [46]. Whether ZIKV affects fecundity or locomotor activity remains controversial [47, 48]. …”

Section: Vector Competence and Vectorial Capacitymentioning

confidence: 99%

Molecular Basis for Arbovirus Transmission by Aedes aegypti Mosquitoes

et al. 2018

View full text Add to dashboard Cite

Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. Examples are dengue, yellow fever, chikungunya, Zika, and Mayaro viruses. After ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. Nevertheless, these arboviruses possess strategies to overcome barriers in the mosquito and eventually reach the salivary glands to continue the transmission cycle. However, the infection and eventual transmission of arbovirus depends on multiple factors. The current review focuses in detail on the anatomic, physiological, and molecular characteristics of the mosquito A. aegypti that participate in response to a viral infection. In the past decades, the awareness of the importance of this mosquito as a disease vector and its impact on human health was largely recognized. We need to improve our comprehension of molecular mechanisms that determine the outcome of successful virus replication or control of infection for each arbovirus in the vector; this could lead to the design of effective control strategies in the future.

show abstract

Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti

Cited by 29 publications

References 62 publications

Zika infection decreases Aedes aegypti locomotor activity but does not influence egg production or viability

Zika infection decreases Aedes aegypti locomotor activity but does not influence egg production or viability

Dengue virus infection changes Aedes aegypti oviposition olfactory preferences

Molecular Basis for Arbovirus Transmission by <b><i>Aedes aegypti</i></b> Mosquitoes

Contact Info

Product

Resources

About