Neurotrophic activity of honokiol on the cultures of fetal rat cortical neurons

Fukuyama, Yoshiyasu; Nakade, Kousuke; Minoshima, Yuka; Yokoyama, Ritsuko; Zhai, Haifeng; Mitsumoto, Yasuhide

doi:10.1016/s0960-894x(02)00112-9

Cited by 92 publications

(50 citation statements)

References 17 publications

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“…This is in agreement with those described for an extract of Magnolia obovata, in which honokiol is thought to be a major effective component (Martínez et al, 2006). Recent evidence indicated that honokiol possessed the neuroprotective eects, and some of these studies suggest that the amelioration of neurotoxicity by honokiol may be attributed to the anti-oxidative and anti-inammatory actions through, at least in part, limiting lipid peroxidation and reducing neutrophil activation/inltration during ischemia and heatstroke (Sheu et al, 2008;Fukuyama et al, 2002). Laboratory studies also revealed that a partially reduced derivative of honokiol, dihydrohonokiol-B (DHH-B; 3'-(2 propenyl)-5-propyl-(1,1'-biphenyl)-2,4'-diol), was also an effective anxiolytic-like agent in mice (Maruyama et al, 2001).…”

Section: Discussionsupporting

confidence: 88%

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation

Ma¹,

Ma²,

Jo³

et al. 2008

Biomolecules and Therapeutics

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− This study was undertaken to investigate whether honokiol could enhance the pentobarbitalinduced sleeping behaviors through γ-aminobutyric acid (GABA) receptor Cl -channel activation. Thirty minutes after the oral administration of honokiol, mice were received sodium pentobarbital (42 mg/kg, i.p.). The time elapsed from pentobarbital injection to the loss of the righting reflex was taken as sleeping latency. The time elapsed between the loss and voluntary recovery of the righting reflex was considered as the total sleeping time. Western blot technique and Cl -sensitive fluorescence probe were used to detect the expression of GABA A receptor subunits and Cl -influx in the primary cultured cerebellar granule cells. Honokiol (0.1 and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg) in a dosage-dependent manner. Honokiol (20 and 50 µM) increased Cl -influx in primary cultured cerebellar granule cells, and selectively increased the GABA A receptor α-subunit expression, but had no effect on the abundance of β or γ-subunits. Chronic treatment with 20 µM honokiol in primary cultured cerebellar neurons did not affect the abundance of GAD65/67. The results suggested that honokiol could potentiate pentobarbital-induced sleeping through GABA A receptor Cl -channel activation.

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Section: Discussionsupporting

confidence: 88%

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation

Ma¹,

Ma²,

Jo³

et al. 2008

Biomolecules and Therapeutics

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“…Honokiol started to capture attention in recent 20 years mainly because of the finding of its promising therapeutic potential to treat multiple human diseases (especially for tumour and thrombus) (Fukuyama et al 2002; Hu et al 2005; Arora et al 2012). Compared with the gradually accumulated knowledge from clinical applications, nevertheless, the understanding of the honokiol’s mode-of-action (MoA) at the molecular levels still remains largely unclear.…”

Section: Introductionmentioning

confidence: 99%

Antifungal compound honokiol triggers oxidative stress responsive signalling pathway and modulates central carbon metabolism

Wang

Yan

2016

Mycology

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The fast growing evidences have shown that the plant-derived compound honokiol is a promising candidate for treating multiple human diseases, such as inflammation and cancer. However, the mode-of-action (MoA) of honokiol remains largely unclear. Here, we studied the antifungal activity of honokiol in fission yeast model, with the goal of understanding the honokiol’s mechanism of action from the molecular level. We found that honokiol can inhibit the yeast growth at a dose-dependent way. Microarray analysis showed that honokiol has wide impacts on the fission yeast transcription levels (in total, 512 genes are up-regulated, and 42 genes are down-regulated). Gene set enrichment analysis indicated that over 45% up-regulated genes belong to the core environmental stress responses category. Moreover, network analysis suggested that there are extensive gene–gene interactions amongst the co-expression gene lists, which can assemble several biofunctionally important modules. It is noteworthy that several key components of central carbon metabolism, such as glucose transporters and metabolic enzymes of glycolysis, are involved in honokiol’s MoA. The complexity of the honokiol’s MoA displayed in previous studies and this work demonstrates that multiple omics approaches and bioinformatics tools should be applied together to achieve the complete scenario of honokiol’s antifungal function.

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“…7) In addition, we reported that magnolol had neurotrophic properties, such as promotion of neurite outgrowth and neuronal survival under serum-deprived conditions in cultured rat cortical neurons. 8) However, these actions have not been demonstrated in in vivo experiments.…”

Section: Magnololmentioning

confidence: 99%

Neurotrophic Effect of Magnolol in the Hippocampal CA1 Region of Senescence-Accelerated Mice (SAMP1)

Matsui

Nakashima

Ushio

et al. 2005

Biological & Pharmaceutical Bulletin

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Magnolol(5,5Ј-di-2-propenyl-1,1Ј-biphenyl-2,2Ј-diol), one of the main constituents in the stem bark of Magnolia obovata THUNB and Magnolia officinalis RHED, 1,2) has been used as a traditional Chinese medicine and has a wide spectrum of pharmacological activities.3) In the central nervous system, magnolol has been reported to increase extracellular acetylcholine release in rat hippocampus, 4) to decrease the release of serotonin, dopamine, and norepinephrine in rat hypothalamus, 5) to reduce the increased production of prostaglandin E 2 caused by chemical hypoxia in neurons, 6) and to interact with g-aminobutyric acid (GABA) receptors in rat brain. 7) In addition, we reported that magnolol had neurotrophic properties, such as promotion of neurite outgrowth and neuronal survival under serum-deprived conditions in cultured rat cortical neurons. 8) However, these actions have not been demonstrated in in vivo experiments.Senescence-accelerated mice (SAM) are selected by brother/sister mating of AKR/J mice, and are characterized by rapid accumulation of senile features and shortened life span (1-1.5 years compared to 2-2.5 years in control animals).9) SAM consist of nine inbred strains of accelerated senescence-prone mice (SAMP) and three of accelerated senescence-resistant mice (SAMR), the latter of which show normal aging. Until the age of 4 months, SAMP1 animals do not differ from SAMR1 either behaviorally or morphologically, but later they begin to rapidly accumulate senile changes. This results in disordering of their behavioral reactions and cognitive brain functions (orientation ability and learning capacity are particularly decreased) and appearance of age-specific morphological changes (lordokyphosis, loss of hair, frequent eye cataract, and mucosal inflammation). 10)Kawaguchi et al. reported that SAMP1TA/Ngs, a substrain of SAMP1, manifests learning disturbance at 7 months of age on a step-down passive avoidance test. 11)This study was undertaken to estimate the neurite outgrowth and neuronal survival effect of magnolol on the senescence-related morphological changes on the density of dendrite in the stratum radiatum of the hippocampal CA1 area, which plays an important role in learning and memory, in SAMP1 mice. MATERIALS AND METHODSMaterials Magnolol (Fig. 1) was isolated from the cortex of M. officinalis RHED. The purity was determined by a high-performance liquid chromatography (single peak) and by nuclear magnetic resonance spectra.Animal Experiments All experiments were conducted in accordance with the Guiding Principles for Care and Use of Laboratory Animals approved by the Japanese Pharmacological Society. The substrains of SAM, SAMP1, and SAMR1 were originally obtained from the Institute for Frontier Medical Sciences in Kyoto University and bred in the Faculty of Health and Welfare Science in Okayama Prefectural University. Mice were kept in an air conditioned room maintained at 25Ϯ1°C with humidity of 55%Ϯ5%. The animals were given food and water ad libitum. Two-to 10-month-old female SAMP1 and SA...

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Neurotrophic activity of honokiol on the cultures of fetal rat cortical neurons

Cited by 92 publications

References 17 publications

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation

Antifungal compound honokiol triggers oxidative stress responsive signalling pathway and modulates central carbon metabolism

Neurotrophic Effect of Magnolol in the Hippocampal CA1 Region of Senescence-Accelerated Mice (SAMP1)

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Neurotrophic activity of honokiol on the cultures of fetal rat cortical neurons

Cited by 92 publications

References 17 publications

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABAAReceptor Cl-Channel Activation

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABAAReceptor Cl-Channel Activation

Antifungal compound honokiol triggers oxidative stress responsive signalling pathway and modulates central carbon metabolism

Neurotrophic Effect of Magnolol in the Hippocampal CA1 Region of Senescence-Accelerated Mice (SAMP1)

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Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation

Honokiol Potentiates Pentobarbital-Induced Sleeping Behaviors through GABA_AReceptor Cl^-Channel Activation