Neurotransmitter Uptake

Hendley, Edith D.

doi:10.1007/978-1-4684-4568-8_15

Cited by 8 publications

(3 citation statements)

References 124 publications

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“…The existence of a sodium-dependent NA transporter in SH-SY5Y cells was demonstrated by the high sodium dependency of [ 3H]NA uptake and the inhibition of that uptake by the specific inhibitor desmethylimipramine (1 pM). Other brain preparations such as synaptosomes and primary cultures give similar results, with typical K. values of 0 .1-2 vM and VmaX values of 1-20 pmol/min/mg of cell protein (reviewed by Hendley, 1984;Kimelberg, 1986) . These values compare very favourably with the Km value of 0 .6 j,M and VmaX value of 17 .8 pmoUmin/mg of protein for SH-SY5Y cells.…”

Section: Intracellular Atp Levelsmentioning

confidence: 70%

Effects of Ischaemic Conditions on Uptake of Glutamate, Aspartate, and Noradrenaline by Cell Lines Derived from the Human Nervous System

O'Neill

Ball

Vaughan

1994

Journal of Neurochemistry

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The effect of hypoglycaemic, hypoxic, and ischaemic conditions on high‐affinity neurotransmitter transport was studied in the human astrocytoma clone D384 and the human neuroblastoma clone SH‐SY5Y. Both cell lines expressed a sodium‐dependent glutamate/aspartate transporter. Km values for d‐[3H]aspartate uptake were 6.1 ± 0.9 µM for D384 cells and 5.3 ± 0.3 µM for SH‐SY5Y cells (mean ± SEM of three experiments). In addition, SH‐SY5Y, but not D384, expressed a sodium‐dependent noradrenaline transporter with Km = 0.6 ± 0.1 µM (mean ± SEM of three experiments). Up to 3 h of hypoglycaemic conditions had no effect on neurotransmitter uptake or on ATP levels of each cell line. In sharp contrast, during hypoxic conditions, the uptake of d‐[3H]aspartate and [3H]noradrenaline declined by 43–56% within 5 min. These reduced rates of neurotransmitter uptake were maintained over 30 min of hypoxic conditions. Five minutes of ischaemic conditions caused similar reductions in neurotransmitter uptake rates. A correlation between reductions in rates of neurotransmitter uptake and in ATP levels was observed for each cell line. Results are discussed in relation to other brain preparations, which are used as models of the nervous system to study the effects of ischaemic conditions on neurotransmitter and energy metabolism.

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Section: Intracellular Atp Levelsmentioning

confidence: 70%

Effects of Ischaemic Conditions on Uptake of Glutamate, Aspartate, and Noradrenaline by Cell Lines Derived from the Human Nervous System

O'Neill

Ball

Vaughan

1994

Journal of Neurochemistry

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“…30 In the meantime the museum had been open "without charge, to visitors of all classes, between dawn and dusk on weekdays". 31 And visitors had come in numbers that exceeded all expectations. In Hendley's final and valedictory museum report to the darbar, written in 1898 on the eve of his departure from Jaipur, he records that the average annual attendance exceeded a quarter of a million people, and that the total for the eleven years since its opening was therefore not far short of three million visitors.…”

Section: The Museum Buildingmentioning

confidence: 99%

The Jaipur Exhibition of 1883

Tillotson¹

2004

J.R. Asiat. Soc. G.B. Irel.

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The exhibition of decorative and industrial arts that was held in Jaipur in 1883 under the patronage of Maharaja Sawai Madho Singh II (1880–1922) brought together the work of artists and craftsmen from many regions of India, but gave special treatment to the neighbouring states of Rajasthan, and to the pupils of Jaipur's own recently established School of Art. It led to the establishment of a permanent museum of industrial arts in Jaipur, which still exists and continues to hold many of the original exhibits. One of many ambitious exhibitions that followed in the wake of the Great Exhibition of 1851, the Jaipur Exhibition was the first such to be held in an Indian state, coinciding with the International Exhibition in Calcutta and preceding the Indian and Colonial Exhibition in London of 1886.

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“…The energy for the transport of NE is derived from the co-transport of Na' down its concentration A TP STIMULATES NOREPINEPHRINE UPTAKE 1513 gradient. The Na+ gradient in turn is maintained by the activity of an intracellular, membrane-bound Na+, K+-ATPase (for review, see Hendley, 1984).…”

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confidence: 99%

Extracellular ATP Stimulates Norepinephrine Uptake in PC12 Cells

Hardwick

Ehrlich

Hendley

1989

Journal of Neurochemistry

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This study examined the effects of extracellular ATP on norepinephrine (NE) uptake, using PC12 cells as a model of noradrenergic neurons. Previous experiments with synaptosomes led to the hypothesis that extracellular ATP can regulate NE uptake via an ecto-protein kinase. In the present study, we examined the high-affinity uptake of NE (referred to as uptake 1) in PC12 cells in the presence of varying concentrations of extracellular ATP. In the presence of Ca2+, low concentrations of ATP (0.1 microM) increased uptake 1 by approximately 36%. This increase could be mimicked by adenosine-5'-O-(3-thiotriphosphate) tetralithium salt (ATP gamma S), an analogue of ATP which can be utilized by protein kinases, and not by 5'-adenylylimidodiphosphate tetralithium salt, a nonhydrolyzable analogue of ATP, GTP, ADP, and adenosine also had no effect on uptake 1. Preincubation of the cells with NE and ATP gamma S, followed by washing and assaying NE uptake 30 min later, resulted in a persistent increase in uptake 1. Similar pretreatment with ATP did not show this increase; however, simultaneous pretreatment with ATP and ATP gamma S blocked the activation produced by ATP gamma S alone. Kinetic analysis showed that ATP gamma S pretreatment produces an increase in the Vmax of uptake 1 without altering the apparent Km for NE. These results support the hypothesis that extracellular ATP can regulate NE uptake via an ecto-protein kinase.

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Neurotransmitter Uptake

Cited by 8 publications

References 124 publications

Effects of Ischaemic Conditions on Uptake of Glutamate, Aspartate, and Noradrenaline by Cell Lines Derived from the Human Nervous System

Effects of Ischaemic Conditions on Uptake of Glutamate, Aspartate, and Noradrenaline by Cell Lines Derived from the Human Nervous System

The Jaipur Exhibition of 1883

Extracellular ATP Stimulates Norepinephrine Uptake in PC12 Cells

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