“…Indeed, this is, to our knowledge, the first study dealing with the comparative toxic potential of tramadol and tapentadol in an in vitro model of human neuronal cells. In this work, we used the undifferentiated neuronal cell line SH-SY5Y, an in vitro model that exhibits many biochemical and functional characteristics of neurons (Valdiglesias et al 2013;Xie et al 2010), and that is frequently used in neurotoxicity studies (Di Daniel et al 2005;Hong et al 2003;Valdiglesias et al 2013 (Caputi et al 2014), low tapentadol concentrations (100 M) do not appear to cause toxicity in the SH-SY5Y cell line in MTT assays (Caputi et al 2014).…”