Neurotoxicity induced in differentiated SK-N-SH-SY5Y human neuroblastoma cells by organophosphorus compounds

Comparative study of the neurotoxicological effects of tramadol and tapentadol in SH-SY5Y cells

“…Indeed, this is, to our knowledge, the first study dealing with the comparative toxic potential of tramadol and tapentadol in an in vitro model of human neuronal cells. In this work, we used the undifferentiated neuronal cell line SH-SY5Y, an in vitro model that exhibits many biochemical and functional characteristics of neurons (Valdiglesias et al 2013;Xie et al 2010), and that is frequently used in neurotoxicity studies (Di Daniel et al 2005;Hong et al 2003;Valdiglesias et al 2013 (Caputi et al 2014), low tapentadol concentrations (100 M) do not appear to cause toxicity in the SH-SY5Y cell line in MTT assays (Caputi et al 2014).…”

Organophosphate Toxicology: Safety Aspects of Nonacetylcholinesterase Secondary Targets

“…CP and its trichloropyridinol hydrolysis product at 3 µM both inhibit neuronal differentiation in PC12 cells by a mechanism independent of AChE inhibition (212). Neurite outgrowth is inhibited by mipafox and OBDPO at 100 µM in PC12 and C6 cells (213) and by mipafox (but not paraoxon) at 1 µM in dorsal root ganglia cell cultures (214) or 50 µM in SH-SY5Y cells (215). In contrast, paraoxon (but not mipafox) (50 µM) alters Ca 2+ homeostasis in these differentiated SH-SY5Y cells (215).…”

A Detoxified Extract ofRhus vernicifluaStokes Upregulated the Expression of BDNF and GDNF in the Rat Brain and the Human Dopaminergic Cell Line SH-SY5Y

“…This cell line is used extensively as a neuron model in many neurobiological, neurochemical, and neurotoxicological studies. [43][44][45][46][47][48] Our findings indicate that RVS is a potent inducer of BDNF and GDNF production in human dopaminergic cells SH-SY5Y, as indicated by enhanced expression of these NTFs. It has been reported that various molecules regulate the expression of BDNF and GDNF in dopamine neurons.…”