Neurospora at the Millennium

Perkins, David; Davis, Rowland H.

doi:10.1006/fgbi.2000.1248

Cited by 30 publications

(9 citation statements)

References 152 publications

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“…In the basidiomycetes Collybia fusipes and Heterobasidon annosum, it was observed that at least three vic loci are involved (Hansen et al, 1993;Marcais and Delatour, 2000). Genetic studies in diVerent ascomycetes have shown that there are several eVectively unlinked vic loci involved: at least 11 in Neurospora crassa (Perkins and Davis, 2000), 9 in Podospora anserina (Saupe et al, 2000), and 6 in Cryphonectria parasitica (Cortesi and Milgroom, 1998). It seems that most vic loci have only two alleles, although multiple alleles have been found in some species (e.g., Saupe and Glass, 1997).…”

Section: Discussionmentioning

confidence: 95%

“…At the molecular level, vegetative incompatibility is probably mediated by the presence of a heterocomplex composed of polypeptides encoded by the alternative vic alleles (Glass et al, 2000). In ascomycetes, genetic studies have shown that there are several unlinked vic loci that regulate the incompatibility response (Cortesi and Milgroom, 1998;Perkins and Davis, 2000;Saupe et al, 2000). Compared to ascomycetes, our knowledge of vegetative incompatibility systems in basidiomycetes is scanty and only a few studies have been conducted.…”

Section: Introductionmentioning

confidence: 98%

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Genetics of self/nonself recognition in Serpula lacrymans

Kauserud¹,

Sætre²,

Schmidt³

et al. 2006

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Genetics of self/nonself recognition in Serpula lacrymans

Kauserud¹,

Sætre²,

Schmidt³

et al. 2006

Fungal Genetics and Biology

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“…The mitotic and meiotic pairing and transmission of homologous chromosomes with length polymorphisms has been studied intensively in models such as the fungi Saccharomyces cerevisiae , N. crassa and Coprinus cinereus [15], [37], [38]. These model systems have substantially increased our knowledge of meiotic processes [39], but they mostly involved cytogenetic studies and mutant strains [40], [41]. A high-density genetic linkage map provides a strong genome-wide alternative for precise analyses of meiosis.…”

Section: Discussionmentioning

confidence: 99%

Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella graminicola

et al. 2009

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Meiosis in the haploid plant-pathogenic fungus Mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. The transient diploid phase allows for recombination among homologous chromosomes. However, some chromosomes of M. graminicola lack homologs and do not pair during meiosis. Because these chromosomes are not present universally in the genome of the organism they can be considered to be dispensable. To analyze the meiotic transmission of unequal chromosome numbers, two segregating populations were generated by crossing genetically unrelated parent isolates originating from Algeria and The Netherlands that had pathogenicity towards durum or bread wheat, respectively. Detailed genetic analyses of these progenies using high-density mapping (1793 DArT, 258 AFLP and 25 SSR markers) and graphical genotyping revealed that M. graminicola has up to eight dispensable chromosomes, the highest number reported in filamentous fungi. These chromosomes vary from 0.39 to 0.77 Mb in size, and represent up to 38% of the chromosomal complement. Chromosome numbers among progeny isolates varied widely, with some progeny missing up to three chromosomes, while other strains were disomic for one or more chromosomes. Between 15–20% of the progeny isolates lacked one or more chromosomes that were present in both parents. The two high-density maps showed no recombination of dispensable chromosomes and hence, their meiotic processing may require distributive disjunction, a phenomenon that is rarely observed in fungi. The maps also enabled the identification of individual twin isolates from a single ascus that shared the same missing or doubled chromosomes indicating that the chromosomal polymorphisms were mitotically stable and originated from nondisjunction during the second division and, less frequently, during the first division of fungal meiosis. High genome plasticity could be among the strategies enabling this versatile pathogen to quickly overcome adverse biotic and abiotic conditions in wheat fields.

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“…It produces better-adapted progenies by driving genetic recombination and eliminating deleterious mutations (Ni et al, 2011; Heitman et al, 2013). Neurospora crassa is a multicellular ascomycete fungus in the family Sordariomycetes and has long been used as an excellent model organism for genetic and biochemical researches as well as the study of morphological development (Springer, 1993; Perkins and Davis, 2000; Davis and Perkins, 2002). From vegetative growth to conidiation or sexual reproduction, morphological changes were evident.…”

Section: Introductionmentioning

confidence: 99%

The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa

Sun

Wang

et al. 2019

Front. Microbiol.

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Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δ ada-6 mutant and wild type during conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δ ada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several conidiation related genes are positively regulated by ADA-6, including genes that positively regulate conidiation ( fluffy and acon-3 ), and genes preferentially expressed during conidial development ( eas , con-6 , con-8 , con-10 , con-13 , pcp-1 , and NCU9357), as the expression of these genes were lower in the Δ ada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2 , poi-2 , and NCU05832, three key genes participating in sexual development. In both conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H 2 O 2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in conidiation, sexual development, and oxidative stress response of N. crassa .

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Neurospora at the Millennium

Cited by 30 publications

References 152 publications

Genetics of self/nonself recognition in Serpula lacrymans

Genetics of self/nonself recognition in Serpula lacrymans

Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella graminicola

The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa

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