Neuroprotector effect of stem cells from human exfoliated deciduous teeth transplanted after traumatic spinal cord injury involves inhibition of early neuronal apoptosis

Nicola, Fabrício do Couto; Marques, M. G.; Odorcyk, Felipe Kawa; Arcego, Danusa Mar; Petenuzzo, Letícia; Aristimunha, D.; Vizuete, Adriana Fernanda Kuckartz; Sanches, Eduardo Farias; Pereira, Denise Rotta Ruttkay; Maurmann, Natasha; Dalmaz, Carla; Pranke, Patrícia; Netto, Carlos Alexandre

doi:10.1016/j.brainres.2017.03.015

Cited by 66 publications

(40 citation statements)

References 56 publications

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“…In the acute phase, the tissue homeostasis has been broken down by the outside injurious stimulation. 61 The dental apical papilla is another available source for SCI therapy. 48 Research has indicated that not only DPSCs but also SHEDs possess the ability to promote locomotor recovery after SCI.…”

Section: Spinal Cord Injurymentioning

confidence: 99%

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Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Wang

Tian

et al. 2019

Cell Proliferation

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Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cellbased therapies, especially those using dental stem cells, bring new hope to neural diseases. Dental stem cells, derived from the neural crest, have many characteristics that are similar to neural cells, indicating that they can be an ideal source of cells for neural regeneration and repair. This review summarizes the neural traits of all the dental cell types, including DPSCs, PDLCs, DFCs, APSCs and their potential applications in nervous system diseases. We have summed up the advantages of dental stem cells in neural repair, such as their neurotrophic and neuroprotective traits, easy harvest and low rejective reaction rate, among others. Taken together, dental stem cells are an ideal cell source for neural tissue regeneration and repair.

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Section: Spinal Cord Injurymentioning

confidence: 99%

“…Cell apoptosis is a major reason for neuronal loss in the AD and SCI disease models 101. In the SCI model, both transfected SHEDs and DPSCs could inhibit early neuronal apoptosis 48,61. In the AD model, experiments showed that DPSCs could reduce apoptosis levels of okadaic acid-induced SH-SY5Y cells 70,[74][75][76].…”

mentioning

confidence: 99%

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Wang

Tian

et al. 2019

Cell Proliferation

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“…More recent studies also focused on their potential neuroprotector effects, showing that SHEDs, after transplantation in a spinal cord injury murine model, were able to induce anti-apoptotic factor activity, resulting in the neuroprotective effects on moto-neurons 36 .…”

Section: Deciduous Teeth and Oral Mucosa: Two Promising Reservoir Of mentioning

confidence: 99%

Human exfoliated deciduous teeth and oral mucosa: promising applications in tissue regeneration

Cristaldi¹,

Mauceri²,

Tomasello³

et al. 2018

J Pediatrics & Pediatr Med

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In the last three decades, the constantly increasing need for therapies, efficiently preventing and/or treating human diseases, has raised the interest in Regenerative Medicine (RM). RM is based on employing mesenchymal stem cells (MSCs), that showed to have great proliferation, self-renewal and multilineage differentiation potential, in vitro as well as in vivo. The opportunity of an accessible, painless and low-cost reservoir of MSCs constitutes the first important step of a successful regenerative therapy to include in the current clinical practice. Oral cavity has recently demonstrated to contain different MSCs niches: dental pulp from permanent and deciduous teeth, periodontal ligament, dental follicle, apical papilla and mucosa. MSCs from dental pulp of deciduous teeth, naturally lost in pediatric age, and the oral mucosa have shown to be easily harvested and to have a promising regenerative potential. Thus, the aim of the paper is to review the potentialities of human exfoliated deciduous teeth stem cells (SHEDs) and oral mucosa stem cells (OMSCs) in RM, with the purpose of their use as accessible source of MSCs for the future of pediatric patient.

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“…Moreover, human DPSCs can differentiate into hepatocytes [7], and the differentiation of DPSCs into pancreatic β cells can be induced by specific culture conditions [8]. Some in vivo experiments in rats have demonstrated that DPSC transplantation can regenerate corneal epithelium [9], myocardium [10], spinal cord [11,12], muscle (in a model of muscular dystrophy) [13], and craniofacial bone (using collagen gel as a scaffold) [14].…”

Section: Introductionmentioning

confidence: 99%

Shikonin induces odontoblastic differentiation of dental pulp stem cells via AKT–mTOR signaling in the presence of CD44

Kajiura

Umemura²,

Ohkoshi

et al. 2020

Preprint

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38In our previous study, we demonstrated that hyaluronan induces odontoblastic 39 differentiation of dental pulp stem cells via interactions with CD44. However, it remains 40 unclear whether CD44 expression by dental pulp stem cells is required for odontoblastic 41 differentiation. Therefore, we searched for a compound that induces odontoblastic 42 differentiation of dental pulp stem cells, regardless of the chemical structure and function 43 of hyaluronan, and examined whether CD44 is involved in the induction of odontoblastic 44 differentiation by the compound. Because vitamin K analogues can promote bone 45 formation and tissue calcification, we focused on derivatives of naphthoquinone, the 46 skeleton of vitamin K; we verified whether those compounds could induce odontoblastic 47 differentiation of dental pulp stem cells. We found that dentin sialophosphoprotein, a 48 marker of odontoblasts, was expressed in dental pulp stem cells after treatment with 49 shikonin. The shikonin-induced expression of dentin sialophosphoprotein was inhibited 50 by PI3K, AKT, and mTOR inhibitors. In addition, in dental pulp stem cells transfected 51 with siRNA against CD44, the shikonin-induced expression of dentin 52 sialophosphoprotein was inhibited. Thus, shikonin can stimulate dental pulp stem cells to 53 undergo odontoblastic differentiation through a mechanism involving the AKT-mTOR 54 signaling pathway and CD44. Hyaluronan stimulated dental pulp stem cells to undergo 55 CD44-mediated odontoblastic differentiation in our previous study; the present study 56 indicated that CD44 is necessary for dental pulp stem cells to undergo odontoblastic 57 differentiation. Although expression of CD44 is important for inducing odontoblastic 58 differentiation of dental pulp stem cells, the relationship between the AKT-mTOR 59 signaling pathway and CD44 expression, in the context of shikonin stimulation, has not 60 yet been elucidated. This study suggested that shikonin may be useful for inducing 61 odontoblastic differentiation of dental pulp stem cells, and that it may have clinical 62 applications, including protection of dental pulp. 63 64 67 CD44 [1]. However, the underlying cellular signaling mechanism has not yet been 68 elucidated, and it has been unclear whether small molecules can induce odontoblastic 69 differentiation of DPSCs. Therefore, we explored whether small molecules could induce 70 odontoblastic differentiation of DPSCs and attempted to identify the differentiation 71 induction mechanism. In addition, we assessed whether CD44 was essential for this 72 mechanism. 73In the field of DPSC research, several research groups have shown that collagenase-74 treated cells isolated from human dental pulp showed more rapid proliferation, compared 75 with hematopoietic stem cells, and a similar degree of multipotency [2,3]. Subsequently, 76 various groups have described possible DPSCs. There have been reports of the induction 77 of odontogenic differentiation from DPSCs in vitro [4-6]. Moreover, human DPSCs can 78 differentiate int...

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Neuroprotector effect of stem cells from human exfoliated deciduous teeth transplanted after traumatic spinal cord injury involves inhibition of early neuronal apoptosis

Cited by 66 publications

References 56 publications

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Human exfoliated deciduous teeth and oral mucosa: promising applications in tissue regeneration

Shikonin induces odontoblastic differentiation of dental pulp stem cells via AKT–mTOR signaling in the presence of CD44

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