Neuroprotective protein hydrolysates from hemp (Cannabis sativa L.) seeds

Abstract

Hemp (Cannabis sativa L.) seeds are well known for their potential use as a source of nutrients, fiber, and bioactive compounds.

Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

“…Other protein hydrolysates isolated from nutritional seeds have also showed anti-inflammatory properties. For instance, the case of hemp seed (Cannabis sativa L.) protein hydrolysates, which inhibited LPS-induced inflammation in the BV2 cell line [6], such as in the case of amaranth (Amaranth hypochondriacus) hydrolysates, which also inhibited LPS-induced inflammation in human and mouse myeloid cells by preventing activation of NF-κB signaling [51]. Our study can improve the efficacy of existing WGPHs that may delay or prevent immune-and inflammatory-related diseases.…”

“…The substitution of animal proteins with plant proteins in the food industry or in our own diet is another important issue for health [2]. Within plant proteins, the study of their enzymatic hydrolysates is of special interest as, they have many bioactive functions, some of which have been shown to exhibit anti-inflammatory, antioxidant, or other bioactive activities [3][4][5][6].…”

Hemp (Cannabis sativa L.) Protein Hydrolysates Promote Anti-Inflammatory Response in Primary Human Monocytes

“…HPPs, that include hemp protein isolate (HPI) and hydrolysates (HPHs), were extendedly explained in preliminary studies as well as their chemical characterization [9]. Briefly, to obtain the HPI, the first step was defatting with n-hexane in a soxhlet, then was performed a first solubilization in alkali at pH 10.5 for 1 h, and, finally, the protein isolate was obtained by precipitation at the isoelectric point (pH 4.3).…”

“…Due to multiple phytochemical compounds hemp seeds have also reached a greater degree of international scientific attention [6][7][8]. In particular, protein represents around 25% of the total hemp seed composition [9]. The major fraction of the total seed protein consists of storage proteins as globulins (65-75%), albumins (25-37%), and sulfur-rich proteins [10].…”

“…Hemp protein hydrolysates (HPHs) generated by enzymatic hydrolysis are composed of polypeptides, oligopeptides, and free amino acids displaying high availability of biological activity [8]. In the literature, HPHs have showed different biological activities as antihypertensive, hypocholesterolemic, antioxidant, antithrombotic, and immunomodulatory effects [8,9,[11][12][13][14][15]. In addition, neuroprotective HPHs have been recently described in preliminary studies by our research team in a microglial BV2 cell line stimulated with lipopolysaccharide (LPS) [9].…”