Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation

Latronico, Tiziana; Larocca, Marilena; Milella, Serafina; Fasano, A.; Rossano, Rocco; Liuzzi, Grazia Maria

doi:10.1007/s10787-020-00772-w

Cited by 27 publications

(23 citation statements)

References 46 publications

(57 reference statements)

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“…Also, AITC inhibited the activation of GFAP responsible for astrogliosis as well as preserved BBB integrity [ 107 ]. In a study that compared the effects of SFN, PEITC, and AITC on neuroinflammation, all ITCs were found to decrease the expression of MMP-2 and MMP-9, though only SFN significantly reduced circulating MMP-2 level via downregulation of ERK phosphorylation [ 291 ]. Interestingly, the effects of AITC upon binding to bitter taste receptor (TAS2R) were shown to be individual specific.…”

Section: Mechanism Of Cardio- and Neuroprotective Effectmentioning

confidence: 99%

Beneficial Health Effects of Glucosinolates-Derived Isothiocyanates on Cardiovascular and Neurodegenerative Diseases

et al. 2022

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Neurodegenerative diseases (NDDs) and cardiovascular diseases (CVDs) are illnesses that affect the nervous system and heart, all of which are vital to the human body. To maintain health of the human body, vegetable diets serve as a preventive approach and particularly Brassica vegetables have been associated with lower risks of chronic diseases, especially NDDs and CVDs. Interestingly, glucosinolates (GLs) and isothiocyanates (ITCs) are phytochemicals that are mostly found in the Cruciferae family and they have been largely documented as antioxidants contributing to both cardio- and neuroprotective effects. The hydrolytic breakdown of GLs into ITCs such as sulforaphane (SFN), phenylethyl ITC (PEITC), moringin (MG), erucin (ER), and allyl ITC (AITC) has been recognized to exert significant effects with regards to cardio- and neuroprotection. From past in vivo and/or in vitro studies, those phytochemicals have displayed the ability to mitigate the adverse effects of reactive oxidation species (ROS), inflammation, and apoptosis, which are the primary causes of CVDs and NDDs. This review focuses on the protective effects of those GL-derived ITCs, featuring their beneficial effects and the mechanisms behind those effects in CVDs and NDDs.

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Section: Mechanism Of Cardio- and Neuroprotective Effectmentioning

confidence: 99%

Beneficial Health Effects of Glucosinolates-Derived Isothiocyanates on Cardiovascular and Neurodegenerative Diseases

et al. 2022

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“…Benzyl isothiocyanate and 6-(Methylsulfinyl)hexyl isothiocyanate derived from Wasabi, have been reported to suppress a transcriptional levels of COX-2, an enzyme synthesizing the pro-inflammatory mediators ( Lee et al, 2009 ; Uto et al, 2012 ). The disturbance of phosphorylated MAPKs signaling, ERK, p38 kinase, and JNK, was observed in the ITCs treatment, resulting in the downregulation of the transcription of inflammatory genes such as COX-2, iNOS, TNF-α, IL-1β, and IL-6 ( Lee et al, 2009 ; Latronico et al, 2021 ). The effects of allyl-isothiocyanate and SFN on the Nrf2 nuclear translocation were associated with the downregulation of p65 protein, a subunit of the transcription factor NF-κB ( Wagner et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Chaiprasongsuk

Panich

2022

Front. Pharmacol.

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Ethnopharmacological studies have become increasingly valuable in the development of botanical products and their bioactive phytochemicals as novel and effective preventive and therapeutic strategies for various diseases including skin photoaging and photodamage-related skin problems including abnormal pigmentation and inflammation. Exploring the roles of phytochemicals in mitigating ultraviolet radiation (UVR)-induced skin damage is thus of importance to offer insights into medicinal and ethnopharmacological potential for development of novel and effective photoprotective agents. UVR plays a role in the skin premature aging (or photoaging) or impaired skin integrity and function through triggering various biological responses of skin cells including apoptosis, oxidative stress, DNA damage and inflammation. In addition, melanin produced by epidermal melanocytes play a protective role against UVR-induced skin damage and therefore hyperpigmentation mediated by UV irradiation could reflect a sign of defensive response of the skin to stress. However, alteration in melanin synthesis may be implicated in skin damage, particularly in individuals with fair skin. Oxidative stress induced by UVR contributes to the process of skin aging and inflammation through the activation of related signaling pathways such as the mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1), the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), the nuclear factor kappa B (NF-κB) and the signal transducer and activator of transcription (STAT) in epidermal keratinocytes and dermal fibroblasts. ROS formation induced by UVR also plays a role in regulation of melanogenesis in melanocytes via modulating MAPK, PI3K/Akt and the melanocortin 1 receptor (MC1R)-microphthalmia-associated transcription factor (MITF) signaling cascades. Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2)-regulated antioxidant defenses can affect the major signaling pathways involved in regulation of photoaging, inflammation associated with skin barrier dysfunction and melanogenesis. This review thus highlights the roles of phytochemicals potentially acting as Nrf2 inducers in improving photoaging, inflammation and hyperpigmentation via regulation of cellular homeostasis involved in skin integrity and function. Taken together, understanding the role of phytochemicals targeting Nrf2 in photoprotection could provide an insight into potential development of natural products as a promising strategy to delay skin photoaging and improve skin conditions.

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“…Detection of intracellular free radicals was performed by loading PBMC (2 × 10 5 cells/well in 96-well plates) or monocytes (2 × 10 5 cells/well in 96-well plates) with 10 µM 2',7'-dichlorofluorescein diacetate in phenol redfree IMDM at 37°C for 30 min (Latronico et al, 2021), then treated for 90 min with ConA (at final concentration of 5 µg/mL), LPS (at final concentration of 10 µg/ mL), or H 2 O 2 (at a final concentration of 1 mM). After incubation, the culture medium was removed and cells were rinsed twice with PBS.…”

Section: Reactive Oxygen Species and Reactive Nitrogen Species Detect...mentioning

confidence: 99%

Oral administration of nucleotides in calves: Effects on oxidative status, immune response, and intestinal mucosa development

Dinardo

Maggiolino

Martinello

et al. 2022

Journal of Dairy Science

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The present work aimed to investigate the effects of nucleotide oral administration on oxidative stress biomarkers, immune responses, gut morphology, serum biochemical parameters, and growth performance in calves from birth to 25 d of life. A total of 40 male Holstein Friesian calves were randomly divided in 2 groups. All the calves were born and reared on the same commercial dairy farm. They were fed the same colostrum, milk replacer, and calf starter. Five grams/head of an additive were orally administered with a syringe directly in the mouth to calves of the nucleotide group (NG). The additive contained 74.12 g/100 g of nucleic acids from hydrolyzed yeast, and 75.38% was free nucleotide sodium salt. The other group represented the negative control (CG). At 25 d of life all of the calves were slaughtered. Calves supplemented with nucleotides had a higher final live weight and improved average daily gain, which was associated with better efficiency of nutrient use. Oral nucleotide administration did not affect IgG absorption efficiency; however, NG calves showed greater duodenum villi length and higher crypt depth compared with CG. Oral nucleotide administration increased the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) and the antioxidant capacity [ferric reducing antioxidant power and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging activity] both in plasma and in liver. An enhanced ability of cells to counter reactive oxygen species-and reactive nitrogen species-mediated damage was also observed in peripheral blood mononuclear cells from NG. The findings highlight the effectiveness of oral nucleotide administration, and potentially dietary supplementation of nucleotides, in boosting oxidative and immune status in newborn calves.

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Neuroprotective potential of isothiocyanates in an in vitro model of neuroinflammation

Cited by 27 publications

References 46 publications

Beneficial Health Effects of Glucosinolates-Derived Isothiocyanates on Cardiovascular and Neurodegenerative Diseases

Beneficial Health Effects of Glucosinolates-Derived Isothiocyanates on Cardiovascular and Neurodegenerative Diseases

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Oral administration of nucleotides in calves: Effects on oxidative status, immune response, and intestinal mucosa development

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