Neuroprotective mechanism of ischemic postconditioning in mice: a possible relationship between protein kinase C and nitric oxide pathways

Gulati, Puja; Singh, Nirmal

doi:10.1016/j.jss.2014.02.019

Cited by 11 publications

(4 citation statements)

References 51 publications

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“…[2] showed that NO donors promote translocation and activation of PKC in an NO and peroxynitrite-dependent fashion. The PKC-mediated mechanism has been related to a neuroprotective effect of NO donors [33]. In the present study, Ni(PipNONO)Cl was also able to increase PKC levels within spinal cord and dura mater, further confirming its ability to activate neuronal NO-mediated pathways.…”

Section: Antidepressant-like Activitysupporting

confidence: 79%

Neuronal effects of a nickel-piperazine/NO donor complex in rodents

Sanna

Monti

Casella

et al. 2015

Pharmacological Research

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Section: Antidepressant-like Activitysupporting

confidence: 79%

Neuronal effects of a nickel-piperazine/NO donor complex in rodents

Sanna

Monti

Casella

et al. 2015

Pharmacological Research

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“…At physiological concentrations (EC 50 1-4 nM) (29,30), NO can regulate blood flow, relax blood vessels, and inhibit platelet aggregation, which are beneficial for the recovery of stroke dysfunction (28,31). Several studies have shown that arginine supplementation contributes to stroke recovery, which is related to the PKC-mediated NO signaling pathway (32). However, recent studies indicate that high concentrations of arginine can increase oxidative stress in the general population (28).…”

Section: Discussionmentioning

confidence: 99%

Targeted Metabolomic Profiling Reveals Association Between Altered Amino Acids and Poor Functional Recovery After Stroke

et al. 2020

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Amino acids have been shown to be among the most important metabolites to be altered following stroke; however, they are a double-edged sword with regard to regulating hemostasis. In this study, we conducted a targeted metabolomic study to examine the association between serum levels of amino acids and functional recovery after stroke. Three hundred and fifty-one patients with stroke admitted to an acute rehabilitation hospital were screened, and 106 patients were selected based on inclusion and exclusion criteria. Recruited patients were stratified using Montebello Rehabilitation Factor Score (MRFS) efficiency. We selected the top (n = 20, 19%) and bottom (n = 20, 19%) of MRFS efficiency for metabolomic analysis. A total of 21 serum amino acids levels were measured using ultra high performance liquid chromatography and mass spectrometry. The normalized data were analyzed by multivariate approaches, and the selected potential biomarkers were combined in different combinations for prediction of stroke functional recovery. The results demonstrated that there were significant differences in leucine-isoleucine, proline, threonine, glutamic acid, and arginine levels between good and poor recovery groups. In the training (0.952) and test (0.835) sets, metabolite biomarker panels composed of proline, glutamic acid, and arginine had the highest sensitivity and specificity in distinguishing good recovery from poor. In particular, arginine was present in the top 10 combinations of the average area under the receiver operating characteristic curve (AUC) test set. Our findings suggest that amino acids related to energy metabolism and excitotoxicity may play an important role in functional recovery after stroke. Therefore, the level of serum arginine has predictive value for the recovery rate after stroke.

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“…It is involved in the oxidative pentose phosphate metabolic pathway and related to myosin light-chain kinase activation ( Sun et al, 2018b ; Geng et al, 2019 ). Protein kinase C (PKC), a family of threonine serine kinases involved in many biological processes, is involved in NOX activation ( Tang et al, 1994 ; Gulati and Singh, 2014 ). Hypoxia-inducible factor-1α (HIF-1α), an critical transcriptional factor regulating hypoxia and ischemia responses ( Yu et al, 2020 ), is activated by PKC and induced hydroxylation and degradation by the enzymes prolyl hydroxylase domain (PHD) ( Gibbs et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Guhong Injection Alleviates Cerebral Ischemia–Reperfusion Injury via the PKC/HIF-1α Pathway in Rats

Zhang

Zhao

et al. 2021

Front. Pharmacol.

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Guhong injection (GHI) is a drug for ischemic stroke created by combining safflower, a traditional Chinese medicine, and aceglutamide, a Western medicine. In this study, we investigated the curative effect of GHI on cerebral ischemia–reperfusion (I/R) injury via the PKC/HIF-1α pathway in rats. Adult male Sprague Dawley rats were randomly divided into seven groups: sham-operated, middle cerebral artery occlusion (MCAO), GHI, nimodipine injection (NMDP), MCAO + LY317615 (PKC inhibitor), GHI + LY317615, and NMDP + LY317615. After establishing an MCAO rat model, we performed neurological deficit testing, 2,3,5-triphenyltetrazolium chloride staining, hematoxylin and eosin (HE) staining, enzyme-linked immunosorbent assay, Western blotting, and q-PCR to detect the brain damage in rats. Compared with the MCAO group, the GHI and GHI + LY317615 group showed neurological damage amelioration as well as decreases in serum hypoxia-inducible factor-1α (HIF-1α), protein kinase C (PKC), and erythropoietin levels; brain HIF-1α and inducible nitric oxide synthase protein expression; and brain HIF-1α and NOX-4 mRNA expression. These effects were similar to those in the positive control groups NMDP and NMDP + LY317615. Thus, our results confirmed GHI can ameliorate cerebral I/R injury in MCAO rats possibly via the PKC/HIF-1α pathway.

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Neuroprotective mechanism of ischemic postconditioning in mice: a possible relationship between protein kinase C and nitric oxide pathways

Cited by 11 publications

References 51 publications

Neuronal effects of a nickel-piperazine/NO donor complex in rodents

Neuronal effects of a nickel-piperazine/NO donor complex in rodents

Targeted Metabolomic Profiling Reveals Association Between Altered Amino Acids and Poor Functional Recovery After Stroke

Guhong Injection Alleviates Cerebral Ischemia–Reperfusion Injury via the PKC/HIF-1α Pathway in Rats

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