Neuroprotective effects of vomifoliol, isolated from Tarenna obtusifolia Merr. (Rubiaceae), against amyloid-beta1-42-treated neuroblastoma SH-SY5Y cells

Tan, Mario A.; Gonzalez, Sebastian Jesus B; Alejandro, Grecebio Jonathan D.; An, Seong Soo A.

doi:10.1007/s13205-020-02421-z

Cited by 10 publications

(7 citation statements)

References 29 publications

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“…Blumenol A ( 3 ) and its isomer (6 S ,9 S )-vomifoliol ( 4 ) demonstrated anti-inflammatory activity in different types of in vitro assays [ 57 , 58 , 59 ]. Recently, neuroprotective function of vomifoliol in amyloid- beta 1-42 -treated neuroblastoma cells has been also studied [ 60 ]. Blumenol A moderately inhibited elongation of shoots and roots of L. sativa cv.…”

Section: Discussionmentioning

confidence: 99%

Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Malarz

Michalska

Stojakowska

2020

Foods

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The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.

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Section: Discussionmentioning

confidence: 99%

Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Malarz

Michalska

Stojakowska

2020

Foods

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“…The procedure for the ThT assay was previously reported [ 2 , 19 , 20 ]. Briefly, Aβ 1-42 (Aggresure™ (AnaSpec) Fremont, CA, USA) was dissolved in phosphate buffered saline (PBS) and incubated with or without the compounds or phenol red (positive control) at 37 °C for 24 h. ThT solution was added and incubated for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability measurement was performed using the ATP luminescence assay as previously described [ 19 , 20 ]. SH-SY5Y cells were counted by Eve cell counter (NanoEntek, Inc., Seoul, Korea), and 2 × 10 4 cells/well density were sub-cultured in 96-well plate and incubated for 24 h. After incubation, cells were treated with the compounds for 24 h. The media were removed, cells were washed with PBS, fresh media were added, and incubated for another 30 min.…”

Section: Methodsmentioning

confidence: 99%

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Tan

Захарова

2021

Biology

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Alzheimer’s disease (AD) remains an incurable neurodegenerative illness. Oxidative stress resulting in the formation of reactive oxygen species (ROS) and the abnormal deposition of amyloid-beta (Aβ) are the major pathological hallmarks associated with AD. In search for small molecules targeting multiple pathways of AD and of no known molecular targets, the neuroprotective effects of the synthetic chromones diaportheone A1 and diaportheone A2, analogues of the natural product diaportheone A, were investigated. Chromones are heterocyclic compounds bearing the benzoannelated γ-pyrone moiety and were regarded as an important class of organic molecules due to their diverse pharmacological activities. The influence of the compounds on the inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and the cell viability, ROS, and mitochondrial membrane potential were evaluated with human neuroblastoma SH-SY5Y cells. Results showed that both compounds inhibited the Aβ aggregation at 80.41% and 73.68% for diaportheone A1 and diaportheone A2, respectively. Increased cell viabilities were observed from the protection by both compounds using Aβ- or H2O2-induced SH-SY5Y cells. Both compounds also reduced the intracellular ROS level in Aβ- or H2O2-induced SH-SY5Y cells at 10 and 20 μM concentrations, and increased the mitochondrial membrane potentials in Aβ-induced SH-SY5Y cells at 20 μM concentration. Molecular docking experiments using the Aβ protein models 2MXU and 2BEG also indicated a good agreement with the experimental data. The results demonstrated for the first time the oxidative stress effects associated with the chromones diaportheone A1 and diaportheone A2 as potential neuroprotective therapeutic agents against AD.

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“…The structures of the new compounds were elucidated through extensive spectroscopic analyses. The known compounds were identified as nudiflopene H (3) [14], latisilinoid (4) [15], (+)-dehydrovomifoliol (5) [16], and vomifoliol (6) [17] by comparing their NMR data with those reported in the literature. Compound 1 was purified as colorless oil.…”

Section: Structure Determinationmentioning

confidence: 99%

Two New Seco-Labdane Diterpenoids from the Leaves of Callicarpa nudiflora

et al. 2022

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Two new seco-labdane diterpenoids, nudiflopene N (1) and nudiflopene O (2), and four known compounds were isolated from the leaves of Callicarpa nudiflora. The structures of the new compounds were established by 1D-, 2D-NMR, and HR-ESI-MS spectral analyses. Compounds 1–3 showed inhibitory activities on lipopolysaccharide-induced nitric oxide (NO) production in RAW264.7 cells, and new compounds 1–2 exhibited more potent inhibitory activity than compound 3. The cytotoxicity of compounds 1–3 against human hepatocellular carcinoma HepG2 cells and human gastric carcinoma SGC-7901 cells were evaluated, while all of them exhibited no cytotoxicity.

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Neuroprotective effects of vomifoliol, isolated from Tarenna obtusifolia Merr. (Rubiaceae), against amyloid-beta1-42-treated neuroblastoma SH-SY5Y cells

Cited by 10 publications

References 29 publications

Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Two New Seco-Labdane Diterpenoids from the Leaves of Callicarpa nudiflora

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